Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
An ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed and validated for confirmatory analysis of four nitroimidazoles and three hydroxy metabolites in honey. Honey samples were dissolved in 2% formic acid solution and nitroimidazoles a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/23/12/3350 |
| _version_ | 1811253630334926848 |
|---|---|
| author | Xiaowei Li Yuebin Ke Yingyu Wang Chengfei Wang Dongyang Ye Xue Hu Lan Zhou Xi Xia |
| author_facet | Xiaowei Li Yuebin Ke Yingyu Wang Chengfei Wang Dongyang Ye Xue Hu Lan Zhou Xi Xia |
| author_sort | Xiaowei Li |
| collection | DOAJ |
| description | An ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed and validated for confirmatory analysis of four nitroimidazoles and three hydroxy metabolites in honey. Honey samples were dissolved in 2% formic acid solution and nitroimidazoles and metabolites were isolated and enriched by dispersive-solid phase extraction using mixed-mode strong cation-exchange sorbent. The determination involves separation of analytes on an UHPLC C<sub>18</sub> column and detection by multiple reaction monitoring in positive ionization mode. The recovery of the method was ranged from 90.2 to 105.6% with inter-day relative standard deviations of less than 11.2%. The limits of detection and limits of quantification were in the ranges of 0.02⁻0.07 µg/kg and 0.05⁻0.2 µg/kg, respectively. Honey samples from the market were analyzed to demonstrate the applicability of the proposed method. |
| first_indexed | 2024-04-12T16:53:42Z |
| format | Article |
| id | doaj.art-4ab24809934a4529af41d2dd221c55eb |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T16:53:42Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4ab24809934a4529af41d2dd221c55eb2022-12-22T03:24:18ZengMDPI AGMolecules1420-30492018-12-012312335010.3390/molecules23123350molecules23123350Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass SpectrometryXiaowei Li0Yuebin Ke1Yingyu Wang2Chengfei Wang3Dongyang Ye4Xue Hu5Lan Zhou6Xi Xia7Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaShenzhen Center for Disease Control and Prevention, Shenzhen 518055, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaAn ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed and validated for confirmatory analysis of four nitroimidazoles and three hydroxy metabolites in honey. Honey samples were dissolved in 2% formic acid solution and nitroimidazoles and metabolites were isolated and enriched by dispersive-solid phase extraction using mixed-mode strong cation-exchange sorbent. The determination involves separation of analytes on an UHPLC C<sub>18</sub> column and detection by multiple reaction monitoring in positive ionization mode. The recovery of the method was ranged from 90.2 to 105.6% with inter-day relative standard deviations of less than 11.2%. The limits of detection and limits of quantification were in the ranges of 0.02⁻0.07 µg/kg and 0.05⁻0.2 µg/kg, respectively. Honey samples from the market were analyzed to demonstrate the applicability of the proposed method.https://www.mdpi.com/1420-3049/23/12/3350nitroimidazolehoneyliquid chromatographymass spectrometryd-SPE |
| spellingShingle | Xiaowei Li Yuebin Ke Yingyu Wang Chengfei Wang Dongyang Ye Xue Hu Lan Zhou Xi Xia Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Molecules nitroimidazole honey liquid chromatography mass spectrometry d-SPE |
| title | Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | confirmatory analysis of nitroimidazoles and hydroxy metabolites in honey by dispersive solid phase extraction and ultra high performance liquid chromatography tandem mass spectrometry |
| topic | nitroimidazole honey liquid chromatography mass spectrometry d-SPE |
| url | https://www.mdpi.com/1420-3049/23/12/3350 |
| work_keys_str_mv | AT xiaoweili confirmatoryanalysisofnitroimidazolesandhydroxymetabolitesinhoneybydispersivesolidphaseextractionandultrahighperformanceliquidchromatographytandemmassspectrometry AT yuebinke confirmatoryanalysisofnitroimidazolesandhydroxymetabolitesinhoneybydispersivesolidphaseextractionandultrahighperformanceliquidchromatographytandemmassspectrometry AT yingyuwang confirmatoryanalysisofnitroimidazolesandhydroxymetabolitesinhoneybydispersivesolidphaseextractionandultrahighperformanceliquidchromatographytandemmassspectrometry AT chengfeiwang confirmatoryanalysisofnitroimidazolesandhydroxymetabolitesinhoneybydispersivesolidphaseextractionandultrahighperformanceliquidchromatographytandemmassspectrometry AT dongyangye confirmatoryanalysisofnitroimidazolesandhydroxymetabolitesinhoneybydispersivesolidphaseextractionandultrahighperformanceliquidchromatographytandemmassspectrometry AT xuehu confirmatoryanalysisofnitroimidazolesandhydroxymetabolitesinhoneybydispersivesolidphaseextractionandultrahighperformanceliquidchromatographytandemmassspectrometry AT lanzhou confirmatoryanalysisofnitroimidazolesandhydroxymetabolitesinhoneybydispersivesolidphaseextractionandultrahighperformanceliquidchromatographytandemmassspectrometry AT xixia confirmatoryanalysisofnitroimidazolesandhydroxymetabolitesinhoneybydispersivesolidphaseextractionandultrahighperformanceliquidchromatographytandemmassspectrometry |