Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress
ABSTRACT: Inhibiting oxidative stress is key for ensuring sperm motility during semen cryopreservation. The aim of this study was to investigate the effect of adding alpha-lipoic acid (ALA) as an extender in rooster semen cryopreservation. Different concentrations of ALA were added to the frozen dil...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124002116 |
| _version_ | 1797248405918973952 |
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| author | Xiaoxin Chen Jianqiang Liu Yi Liu Xu Li Dingjie An Xiaohui Liu Lichun Zhang |
| author_facet | Xiaoxin Chen Jianqiang Liu Yi Liu Xu Li Dingjie An Xiaohui Liu Lichun Zhang |
| author_sort | Xiaoxin Chen |
| collection | DOAJ |
| description | ABSTRACT: Inhibiting oxidative stress is key for ensuring sperm motility during semen cryopreservation. The aim of this study was to investigate the effect of adding alpha-lipoic acid (ALA) as an extender in rooster semen cryopreservation. Different concentrations of ALA were added to the frozen diluent of rooster semen; subsequently, computer-aided semen analysis was used to determine membrane functional integrity, acrosome integrity, antioxidant capacity (based on T-AOC, GSH-Px, SOD, CAT, and MDA contents), and mitochondrial integrity. The frozen sperm ultrastructure was observed using transmission electron microscopy. The results showed that the addition of different concentrations of ALA partially to greatly improved the quality of frozen sperm; in particular, 8 μg/mL ALA significantly improved multiple parameters of sperm quality, including sperm motility and antioxidant enzyme activity, after freeze–thaw. The results of this study provide empirical and theoretical support for effective rooster semen cryopreservation and can inform the development of new protective agents in the field of livestock reproduction. |
| first_indexed | 2024-04-24T20:14:04Z |
| format | Article |
| id | doaj.art-4ab358f78fa34901a6462f65aecbf6dd |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T20:14:04Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-4ab358f78fa34901a6462f65aecbf6dd2024-03-23T06:21:54ZengElsevierPoultry Science0032-57912024-05-011035103632Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stressXiaoxin Chen0Jianqiang Liu1Yi Liu2Xu Li3Dingjie An4Xiaohui Liu5Lichun Zhang6Institute of Animal Sciences, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, ChinaInstitute of Animal Sciences, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, ChinaInstitute of Animal Sciences, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, ChinaInstitute of Animal Sciences, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, ChinaInstitute of Animal Sciences, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, ChinaInstitute of Animal Sciences, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, ChinaCorresponding author:; Institute of Animal Sciences, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, ChinaABSTRACT: Inhibiting oxidative stress is key for ensuring sperm motility during semen cryopreservation. The aim of this study was to investigate the effect of adding alpha-lipoic acid (ALA) as an extender in rooster semen cryopreservation. Different concentrations of ALA were added to the frozen diluent of rooster semen; subsequently, computer-aided semen analysis was used to determine membrane functional integrity, acrosome integrity, antioxidant capacity (based on T-AOC, GSH-Px, SOD, CAT, and MDA contents), and mitochondrial integrity. The frozen sperm ultrastructure was observed using transmission electron microscopy. The results showed that the addition of different concentrations of ALA partially to greatly improved the quality of frozen sperm; in particular, 8 μg/mL ALA significantly improved multiple parameters of sperm quality, including sperm motility and antioxidant enzyme activity, after freeze–thaw. The results of this study provide empirical and theoretical support for effective rooster semen cryopreservation and can inform the development of new protective agents in the field of livestock reproduction.http://www.sciencedirect.com/science/article/pii/S0032579124002116alpha-lipoic acidantioxidant capacitysemen freezingrooster sperm ultramicrostructure |
| spellingShingle | Xiaoxin Chen Jianqiang Liu Yi Liu Xu Li Dingjie An Xiaohui Liu Lichun Zhang Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress Poultry Science alpha-lipoic acid antioxidant capacity semen freezing rooster sperm ultramicrostructure |
| title | Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress |
| title_full | Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress |
| title_fullStr | Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress |
| title_full_unstemmed | Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress |
| title_short | Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress |
| title_sort | alpha lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress |
| topic | alpha-lipoic acid antioxidant capacity semen freezing rooster sperm ultramicrostructure |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124002116 |
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