Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism
Abstract In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12 n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared wit...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-02-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-020-0956-5 |
| _version_ | 1818642954902831104 |
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| author | Jia-Heng Ji Lei Zhou Serge Maurice Mbadinga Muhammad Irfan Yi-Fan Liu Pan Pan Zhen-Zhen Qi Jing Chen Jin-Feng Liu Shi-Zhong Yang Ji-Dong Gu Bo-Zhong Mu |
| author_facet | Jia-Heng Ji Lei Zhou Serge Maurice Mbadinga Muhammad Irfan Yi-Fan Liu Pan Pan Zhen-Zhen Qi Jing Chen Jin-Feng Liu Shi-Zhong Yang Ji-Dong Gu Bo-Zhong Mu |
| author_sort | Jia-Heng Ji |
| collection | DOAJ |
| description | Abstract In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12 n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C9 to C12 alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions. |
| first_indexed | 2024-12-16T23:51:16Z |
| format | Article |
| id | doaj.art-4ab38fff3cc24d12890c3be74df6e5ad |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-16T23:51:16Z |
| publishDate | 2020-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-4ab38fff3cc24d12890c3be74df6e5ad2022-12-21T22:11:19ZengSpringerOpenAMB Express2191-08552020-02-011011910.1186/s13568-020-0956-5Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanismJia-Heng Ji0Lei Zhou1Serge Maurice Mbadinga2Muhammad Irfan3Yi-Fan Liu4Pan Pan5Zhen-Zhen Qi6Jing Chen7Jin-Feng Liu8Shi-Zhong Yang9Ji-Dong Gu10Bo-Zhong Mu11State Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologySchool of Biological Sciences, The University of Hong KongState Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and TechnologyAbstract In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12 n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C9 to C12 alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.https://doi.org/10.1186/s13568-020-0956-5AlkanesAlkylsuccinatesFumarate additionMethanogenesisOil reservoirs |
| spellingShingle | Jia-Heng Ji Lei Zhou Serge Maurice Mbadinga Muhammad Irfan Yi-Fan Liu Pan Pan Zhen-Zhen Qi Jing Chen Jin-Feng Liu Shi-Zhong Yang Ji-Dong Gu Bo-Zhong Mu Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism AMB Express Alkanes Alkylsuccinates Fumarate addition Methanogenesis Oil reservoirs |
| title | Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism |
| title_full | Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism |
| title_fullStr | Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism |
| title_full_unstemmed | Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism |
| title_short | Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism |
| title_sort | methanogenic biodegradation of c9 to c12 n alkanes initiated by smithella via fumarate addition mechanism |
| topic | Alkanes Alkylsuccinates Fumarate addition Methanogenesis Oil reservoirs |
| url | https://doi.org/10.1186/s13568-020-0956-5 |
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