Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments

Uppermost-internode diameter (UID) is a key morphological trait associated with spike development and yield potential in wheat. Our understanding of its genetic basis remains largely unknown. Here, quantitative trait loci (QTLs) for UID with high-density genetic maps were identified in five wheat re...

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Main Authors: Hang LIU, Hua-ping TANG, Wei LUO, Yang MU, Qian-tao JIANG, Ya-xi LIU, Guo-yue CHEN, Ji-rui WANG, Zhi ZHENG, Peng-fei QI, Yun-feng JIANG, Fa CUI, Yin-ming SONG, Gui-jun YAN, Yu-ming WEI, Xiu-jin LAN, You-liang ZHENG, Jian MA
Format: Article
Language:English
Published: Elsevier 2021-11-01
Series:Journal of Integrative Agriculture
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311920634128
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author Hang LIU
Hua-ping TANG
Wei LUO
Yang MU
Qian-tao JIANG
Ya-xi LIU
Guo-yue CHEN
Ji-rui WANG
Zhi ZHENG
Peng-fei QI
Yun-feng JIANG
Fa CUI
Yin-ming SONG
Gui-jun YAN
Yu-ming WEI
Xiu-jin LAN
You-liang ZHENG
Jian MA
author_facet Hang LIU
Hua-ping TANG
Wei LUO
Yang MU
Qian-tao JIANG
Ya-xi LIU
Guo-yue CHEN
Ji-rui WANG
Zhi ZHENG
Peng-fei QI
Yun-feng JIANG
Fa CUI
Yin-ming SONG
Gui-jun YAN
Yu-ming WEI
Xiu-jin LAN
You-liang ZHENG
Jian MA
author_sort Hang LIU
collection DOAJ
description Uppermost-internode diameter (UID) is a key morphological trait associated with spike development and yield potential in wheat. Our understanding of its genetic basis remains largely unknown. Here, quantitative trait loci (QTLs) for UID with high-density genetic maps were identified in five wheat recombinant inbred line (RIL) populations. In total, 25 QTLs for UID were detected in five RIL populations, and they were located on chromosomes 1A, 1D (3 QTL), 2B (2), 2D (3), 3B, 3D, 4A, 4B (3), 4D, 5A (5), 5B (2), 6B, and 7D. Of them, five major and stable QTLs (QUid.sau-2CN-1D.1, QUid.sau-2SY-1D, QUid.sau-QZ-2D, QUid.sau-SC-3D, and QUid.sau-AS-4B) were identified from each of the five RIL populations in multiple environments. QUid.sau-2CN-1D.1, QUid.sau-2SY-1D and QUid.sau-SC-3D are novel QTLs. Kompetitive Allele Specific PCR (KASP) markers tightly linked to them were further investigated for developing near-isogenic lines (NILs) carrying the major loci. Furthermore, candidate genes at these intervals harboring major and stable QTLs were predicted, and they were associated with plant development and water transportation in most cases. Comparison of physical locations of the identified QTL on the ‘Chinese Spring’ reference genome showed that several QTLs including two major ones, QUid.sau-2CN-1D.1 and QUid.sau-2SY-1D, are likely allelic confirming their validity and effectiveness. The significant relationships detected between UID and other agronomic traits and a proper UID were discussed. Collectively, our results dissected the underlying genetic basis for UID in wheat and laid a foundation for further fine mapping and map-based cloning of these QTLs.
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spelling doaj.art-4ab47d0d79fe410794b04e67369cf9e32022-12-21T20:00:53ZengElsevierJournal of Integrative Agriculture2095-31192021-11-01201128492861Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environmentsHang LIU0Hua-ping TANG1Wei LUO2Yang MU3Qian-tao JIANG4Ya-xi LIU5Guo-yue CHEN6Ji-rui WANG7Zhi ZHENG8Peng-fei QI9Yun-feng JIANG10Fa CUI11Yin-ming SONG12Gui-jun YAN13Yu-ming WEI14Xiu-jin LAN15You-liang ZHENG16Jian MA17State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaAgriculture and Food, CSIRO, St Lucia, Brisbane 4217, AustraliaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaCollege of Agriculture, Ludong University, Yantai 264000, P.R.ChinaCollege of Agronomy and Biotechnology, China Agricultural University, Beijing 100093, P.R.ChinaSchool of Plant Biology, The University of Western Australia, Perth WA 6009, AustraliaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China; Correspondence MA Jian, Tel: +86-28-86293115, Fax: +86-28-82650350Uppermost-internode diameter (UID) is a key morphological trait associated with spike development and yield potential in wheat. Our understanding of its genetic basis remains largely unknown. Here, quantitative trait loci (QTLs) for UID with high-density genetic maps were identified in five wheat recombinant inbred line (RIL) populations. In total, 25 QTLs for UID were detected in five RIL populations, and they were located on chromosomes 1A, 1D (3 QTL), 2B (2), 2D (3), 3B, 3D, 4A, 4B (3), 4D, 5A (5), 5B (2), 6B, and 7D. Of them, five major and stable QTLs (QUid.sau-2CN-1D.1, QUid.sau-2SY-1D, QUid.sau-QZ-2D, QUid.sau-SC-3D, and QUid.sau-AS-4B) were identified from each of the five RIL populations in multiple environments. QUid.sau-2CN-1D.1, QUid.sau-2SY-1D and QUid.sau-SC-3D are novel QTLs. Kompetitive Allele Specific PCR (KASP) markers tightly linked to them were further investigated for developing near-isogenic lines (NILs) carrying the major loci. Furthermore, candidate genes at these intervals harboring major and stable QTLs were predicted, and they were associated with plant development and water transportation in most cases. Comparison of physical locations of the identified QTL on the ‘Chinese Spring’ reference genome showed that several QTLs including two major ones, QUid.sau-2CN-1D.1 and QUid.sau-2SY-1D, are likely allelic confirming their validity and effectiveness. The significant relationships detected between UID and other agronomic traits and a proper UID were discussed. Collectively, our results dissected the underlying genetic basis for UID in wheat and laid a foundation for further fine mapping and map-based cloning of these QTLs.http://www.sciencedirect.com/science/article/pii/S2095311920634128uppermost-internode diameterwheatyield potentialgenetic basisquantitative trait loci
spellingShingle Hang LIU
Hua-ping TANG
Wei LUO
Yang MU
Qian-tao JIANG
Ya-xi LIU
Guo-yue CHEN
Ji-rui WANG
Zhi ZHENG
Peng-fei QI
Yun-feng JIANG
Fa CUI
Yin-ming SONG
Gui-jun YAN
Yu-ming WEI
Xiu-jin LAN
You-liang ZHENG
Jian MA
Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
Journal of Integrative Agriculture
uppermost-internode diameter
wheat
yield potential
genetic basis
quantitative trait loci
title Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
title_full Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
title_fullStr Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
title_full_unstemmed Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
title_short Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
title_sort genetic dissection of wheat uppermost internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
topic uppermost-internode diameter
wheat
yield potential
genetic basis
quantitative trait loci
url http://www.sciencedirect.com/science/article/pii/S2095311920634128
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