Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench)
Application of high levels of chemical fertilizers for optimal growth of sweet sorghum causes environmental degradation. Plant growth‐promoting bacteria have biotechnological importance because they can improve the growth and health of important agronomic plants. This study aimed to isolate, charact...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2021-12-01
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| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/64893 |
| _version_ | 1811170996155056128 |
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| author | Charlie Ester de Fretes Donny Widianto Yekti Asih Purwestri Tri Rini Nuringtyas |
| author_facet | Charlie Ester de Fretes Donny Widianto Yekti Asih Purwestri Tri Rini Nuringtyas |
| author_sort | Charlie Ester de Fretes |
| collection | DOAJ |
| description | Application of high levels of chemical fertilizers for optimal growth of sweet sorghum causes environmental degradation. Plant growth‐promoting bacteria have biotechnological importance because they can improve the growth and health of important agronomic plants. This study aimed to isolate, characterize, and identify endophytic bacteria associated with sweet sorghum (cv. KCS105), and also to study the inoculation effects of selected isolates on sorghum growth. In this study, 35 isolates were evaluated for their ability to support plant growth. The results showed that seven isolates were diazotrophic, six were capable of dissolving phosphate, six produced IAA and could detect ACC‐deaminase activity, and three inhibited the growth of pathogenic fungi. Nine isolates exhibiting mechanisms for promoting plant growth from the Alphaproteobacteria (Devosia), Firmicutes (Bacillus, Paenibacillus, Staphylococcus), and Actinobacteria (Microbacterium, Brachybacterium) phyla were identified. In addition, the Paenibacillus sp. BB7, Bacillus sp. PIB1B, and Bacillus sp. PLB1B isolates showed increasing effects on plant growth in greenhouse tests. Endophytic bacterial isolates which display plant growth‐promoting features can potentially be employed as biofertilizer agents. They may also address environmental damage problems resulting from the use of chemical fertilizers and pesticides. |
| first_indexed | 2024-04-10T17:06:08Z |
| format | Article |
| id | doaj.art-4ab781b9978a415881cb19b419f5652e |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-10T17:06:08Z |
| publishDate | 2021-12-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-4ab781b9978a415881cb19b419f5652e2023-02-06T03:00:18ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412021-12-0126419019610.22146/ijbiotech.6489331123Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench)Charlie Ester de Fretes0Donny Widianto1Yekti Asih Purwestri2Tri Rini Nuringtyas3Research Center for Deep Sea, National Research and Innovation Agency, Jl. Y. Syaranamual, Ambon, Maluku, 97123, IndonesiaResearch Center for Biotechnology, Universitas Gadjah Mada, Jl. Teknika Utara, Barek, Yogyakarta, 55281, Indonesia; Department of Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta, 55281, IndonesiaResearch Center for Biotechnology, Universitas Gadjah Mada, Jl. Teknika Utara, Barek, Yogyakarta, 55281, Indonesia; Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, IndonesiaResearch Center for Biotechnology, Universitas Gadjah Mada, Jl. Teknika Utara, Barek, Yogyakarta, 55281, Indonesia; Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, IndonesiaApplication of high levels of chemical fertilizers for optimal growth of sweet sorghum causes environmental degradation. Plant growth‐promoting bacteria have biotechnological importance because they can improve the growth and health of important agronomic plants. This study aimed to isolate, characterize, and identify endophytic bacteria associated with sweet sorghum (cv. KCS105), and also to study the inoculation effects of selected isolates on sorghum growth. In this study, 35 isolates were evaluated for their ability to support plant growth. The results showed that seven isolates were diazotrophic, six were capable of dissolving phosphate, six produced IAA and could detect ACC‐deaminase activity, and three inhibited the growth of pathogenic fungi. Nine isolates exhibiting mechanisms for promoting plant growth from the Alphaproteobacteria (Devosia), Firmicutes (Bacillus, Paenibacillus, Staphylococcus), and Actinobacteria (Microbacterium, Brachybacterium) phyla were identified. In addition, the Paenibacillus sp. BB7, Bacillus sp. PIB1B, and Bacillus sp. PLB1B isolates showed increasing effects on plant growth in greenhouse tests. Endophytic bacterial isolates which display plant growth‐promoting features can potentially be employed as biofertilizer agents. They may also address environmental damage problems resulting from the use of chemical fertilizers and pesticides.https://jurnal.ugm.ac.id/ijbiotech/article/view/64893endophytic bacteriaplant growth‐promoting bacteriasweet sorghum16s rrna gene |
| spellingShingle | Charlie Ester de Fretes Donny Widianto Yekti Asih Purwestri Tri Rini Nuringtyas Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench) Indonesian Journal of Biotechnology endophytic bacteria plant growth‐promoting bacteria sweet sorghum 16s rrna gene |
| title | Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench) |
| title_full | Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench) |
| title_fullStr | Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench) |
| title_full_unstemmed | Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench) |
| title_short | Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench) |
| title_sort | plant growth promoting activity of endophytic bacteria from sweet sorghum sorghum bicolor l moench |
| topic | endophytic bacteria plant growth‐promoting bacteria sweet sorghum 16s rrna gene |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/64893 |
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