Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice
New generation vaccines, particularly those based on recombinant proteins, are generally less reactogenic than traditional live attenuated vaccines. Nevertheless, in terms of immunogenicity, they require potent adjuvants to reach a proper immune response in the recipients. We had previously evaluate...
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| Format: | Article |
| Language: | English |
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Elfos Scientiae
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| Series: | Biotecnología Aplicada |
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| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522009000300003&lng=en&tlng=en |
| _version_ | 1819023005253107712 |
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| author | Iris Valdés Olivia Niebla Lisset Hermida Jorge Sánchez Laura Lazo Jorge Martín Yaremis Romero Yadira Rodríguez Maria G Guzmán Gerardo |
| author_facet | Iris Valdés Olivia Niebla Lisset Hermida Jorge Sánchez Laura Lazo Jorge Martín Yaremis Romero Yadira Rodríguez Maria G Guzmán Gerardo |
| author_sort | Iris Valdés |
| collection | DOAJ |
| description | New generation vaccines, particularly those based on recombinant proteins, are generally less reactogenic than traditional live attenuated vaccines. Nevertheless, in terms of immunogenicity, they require potent adjuvants to reach a proper immune response in the recipients. We had previously evaluated the potential capacity of PD5 protein (a vaccine candidate against dengue-2, composed by the P64k protein of Neisseria meningitidis, and the domain III of the dengue Envelope protein), as a vaccine candidate with Freund’s adjuvant. In this work, we evaluated the adjuvant capacity of the outer membrane vesicles (OMV) from N. meningitidis on the immunogenicity of the PD5 protein. As a result, after three doses in mice, the groups immunized with three different formulations of OMV elicited high titers of antiviral and neutralizing antibodies against dengue-2 with predominant IgG1 levels. Additionally, in the protection study, the most statistical difference was obtained in one of the three groups immunized with OMV, specifically with one formulation which favors the possible association between the protein and vesicles. |
| first_indexed | 2024-12-21T04:32:01Z |
| format | Article |
| id | doaj.art-4ab7d15b72e0479fad681899413fcece |
| institution | Directory Open Access Journal |
| issn | 1027-2852 |
| language | English |
| last_indexed | 2024-12-21T04:32:01Z |
| publisher | Elfos Scientiae |
| record_format | Article |
| series | Biotecnología Aplicada |
| spelling | doaj.art-4ab7d15b72e0479fad681899413fcece2022-12-21T19:15:55ZengElfos ScientiaeBiotecnología Aplicada1027-2852263209213S1027-28522009000300003Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in miceIris Valdés0Olivia Niebla1Lisset Hermida2Jorge Sánchez3Laura Lazo4Jorge Martín5Yaremis Romero6Yadira Rodríguez7Maria G Guzmán8Gerardo9International Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyPedro Kourí Tropical Medicine InstituteInternational Centre for Genetic Engineering and BiotechnologyNew generation vaccines, particularly those based on recombinant proteins, are generally less reactogenic than traditional live attenuated vaccines. Nevertheless, in terms of immunogenicity, they require potent adjuvants to reach a proper immune response in the recipients. We had previously evaluated the potential capacity of PD5 protein (a vaccine candidate against dengue-2, composed by the P64k protein of Neisseria meningitidis, and the domain III of the dengue Envelope protein), as a vaccine candidate with Freund’s adjuvant. In this work, we evaluated the adjuvant capacity of the outer membrane vesicles (OMV) from N. meningitidis on the immunogenicity of the PD5 protein. As a result, after three doses in mice, the groups immunized with three different formulations of OMV elicited high titers of antiviral and neutralizing antibodies against dengue-2 with predominant IgG1 levels. Additionally, in the protection study, the most statistical difference was obtained in one of the three groups immunized with OMV, specifically with one formulation which favors the possible association between the protein and vesicles.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522009000300003&lng=en&tlng=enadyuvantevirus dengueneisseria meningitidisvesículas de membrana externaproteína recombinante |
| spellingShingle | Iris Valdés Olivia Niebla Lisset Hermida Jorge Sánchez Laura Lazo Jorge Martín Yaremis Romero Yadira Rodríguez Maria G Guzmán Gerardo Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice Biotecnología Aplicada adyuvante virus dengue neisseria meningitidis vesículas de membrana externa proteína recombinante |
| title | Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice |
| title_full | Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice |
| title_fullStr | Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice |
| title_full_unstemmed | Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice |
| title_short | Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice |
| title_sort | evaluation of different formulations of a dengue 2 chimeric protein and outer membrane vesicles from neisseria meningitidis in mice |
| topic | adyuvante virus dengue neisseria meningitidis vesículas de membrana externa proteína recombinante |
| url | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522009000300003&lng=en&tlng=en |
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