Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application
Silver nanoparticles (AgNPs) were synthesized using hydroalcoholic extracts of dittany (<i>Origanum dictamnus</i>), sage (<i>Salvia officinalis</i>), sea buckthorn (<i>Elaeagnus rhamnoides</i>, syn. <i>Hippophae rhamnoides</i>), and calendula (<i>...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/12/1244 |
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| author | Ioanna K. Siakavella Fotini Lamari Dimitrios Papoulis Malvina Orkoula Patroula Gkolfi Michail Lykouras Konstantinos Avgoustakis Sophia Hatziantoniou |
| author_facet | Ioanna K. Siakavella Fotini Lamari Dimitrios Papoulis Malvina Orkoula Patroula Gkolfi Michail Lykouras Konstantinos Avgoustakis Sophia Hatziantoniou |
| author_sort | Ioanna K. Siakavella |
| collection | DOAJ |
| description | Silver nanoparticles (AgNPs) were synthesized using hydroalcoholic extracts of dittany (<i>Origanum dictamnus</i>), sage (<i>Salvia officinalis</i>), sea buckthorn (<i>Elaeagnus rhamnoides</i>, syn. <i>Hippophae rhamnoides</i>), and calendula (<i>Calendula officinalis</i>) as reducing agents. AgNPs synthesized using NaBH<sub>4</sub> and citric acid were used as control. The impact of the origin of the extract and preparation conditions (light, temperature, reaction time) on the properties of the synthesized AgNPs was investigated. The structure, morphology, composition, physicochemical characteristics, and colloidal stability were characterized using dynamic laser scattering (DLS), ultraviolet-visible spectrophotometry (UV–/Vis), XRD, X-ray fluorescence (XRF), TEM, and FTΙR. The reduction of total phenolic and flavonoid content of the extracts after the reaction of AgNPs synthesis was also determined. Low IC50 values for all types of AgNPs revealed good antioxidant activity, attributable to the phenolic and flavonoid content of their surface. The results suggest that plant extract selection is important to the green synthesis of AgNPs because it affects the kinetics of their synthesis as well as their morphology, physicochemical characteristics, and colloidal stability. In vitro permeation studies on porcine skin revealed that AgNPs remained at the upper layers of stratum corneum and did not penetrate the skin barrier after 4 h of cutaneous application suggesting the safety of their application on intact skin for a relatively short time. |
| first_indexed | 2024-03-10T13:53:23Z |
| format | Article |
| id | doaj.art-4abe51c27f3e4dbeaceed72c20b011b2 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T13:53:23Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-4abe51c27f3e4dbeaceed72c20b011b22023-11-21T01:52:21ZengMDPI AGPharmaceutics1999-49232020-12-011212124410.3390/pharmaceutics12121244Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical ApplicationIoanna K. Siakavella0Fotini Lamari1Dimitrios Papoulis2Malvina Orkoula3Patroula Gkolfi4Michail Lykouras5Konstantinos Avgoustakis6Sophia Hatziantoniou7Laboratory of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, GreeceLaboratory of Pharmacognosy & Chemistry of Natural Products Department of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, GreeceDepartment of Geology, University of Patras, 26504 Patras, GreeceLaboratory of Instrumental Pharmaceutical Analysis, Department of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, GreeceDepartment of Chemistry, University of Patras, 26504 Patras, GreeceLaboratory of Instrumental Pharmaceutical Analysis, Department of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, GreeceLaboratory of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, GreeceLaboratory of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, GreeceSilver nanoparticles (AgNPs) were synthesized using hydroalcoholic extracts of dittany (<i>Origanum dictamnus</i>), sage (<i>Salvia officinalis</i>), sea buckthorn (<i>Elaeagnus rhamnoides</i>, syn. <i>Hippophae rhamnoides</i>), and calendula (<i>Calendula officinalis</i>) as reducing agents. AgNPs synthesized using NaBH<sub>4</sub> and citric acid were used as control. The impact of the origin of the extract and preparation conditions (light, temperature, reaction time) on the properties of the synthesized AgNPs was investigated. The structure, morphology, composition, physicochemical characteristics, and colloidal stability were characterized using dynamic laser scattering (DLS), ultraviolet-visible spectrophotometry (UV–/Vis), XRD, X-ray fluorescence (XRF), TEM, and FTΙR. The reduction of total phenolic and flavonoid content of the extracts after the reaction of AgNPs synthesis was also determined. Low IC50 values for all types of AgNPs revealed good antioxidant activity, attributable to the phenolic and flavonoid content of their surface. The results suggest that plant extract selection is important to the green synthesis of AgNPs because it affects the kinetics of their synthesis as well as their morphology, physicochemical characteristics, and colloidal stability. In vitro permeation studies on porcine skin revealed that AgNPs remained at the upper layers of stratum corneum and did not penetrate the skin barrier after 4 h of cutaneous application suggesting the safety of their application on intact skin for a relatively short time.https://www.mdpi.com/1999-4923/12/12/1244silver nanoparticlesAgNPgreen chemistryplant extractstopical applicationskin permeation |
| spellingShingle | Ioanna K. Siakavella Fotini Lamari Dimitrios Papoulis Malvina Orkoula Patroula Gkolfi Michail Lykouras Konstantinos Avgoustakis Sophia Hatziantoniou Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application Pharmaceutics silver nanoparticles AgNP green chemistry plant extracts topical application skin permeation |
| title | Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application |
| title_full | Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application |
| title_fullStr | Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application |
| title_full_unstemmed | Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application |
| title_short | Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application |
| title_sort | effect of plant extracts on the characteristics of silver nanoparticles for topical application |
| topic | silver nanoparticles AgNP green chemistry plant extracts topical application skin permeation |
| url | https://www.mdpi.com/1999-4923/12/12/1244 |
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