CDC5L Promotes hTERT Expression and Colorectal Tumor Growth
Background/Aims: Colorectal cancer (CRC) is the third leading cause of cancer-related death worldwide because the survival rate remains low. Cell division cycle 5-like (CDC5L) is highly expressed in some cancer cells, but the mechanism requires clarification. Human telomerase reverse transcriptase (...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2017-05-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/475916 |
| _version_ | 1818318067750404096 |
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| author | Jia Li Ningning Zhang Rui Zhang Longmei Sun Wendan Yu Wei Guo Yingying Gao Mei Li Wei Liu Pin Liang Wuguo Deng Xiaonan Cui |
| author_facet | Jia Li Ningning Zhang Rui Zhang Longmei Sun Wendan Yu Wei Guo Yingying Gao Mei Li Wei Liu Pin Liang Wuguo Deng Xiaonan Cui |
| author_sort | Jia Li |
| collection | DOAJ |
| description | Background/Aims: Colorectal cancer (CRC) is the third leading cause of cancer-related death worldwide because the survival rate remains low. Cell division cycle 5-like (CDC5L) is highly expressed in some cancer cells, but the mechanism requires clarification. Human telomerase reverse transcriptase (hTERT) plays important roles in CRC. Methods: This study aimed to identify a link between CDC5L and hTERT and to determine their effects on the signaling pathways, migration and prognosis of CRC cells. We first treated LoVo cells with biotin-labeled hTERT and identified CDC5L. Then, pulldown and ChIP assays were used to verify whether CDC5L was a promoter of hTERT. The roles of CDC5L and hTERT in cell growth and migration were studied using siRNA in vivo and in vitro. 130 human CRC specimens were analyzed using immunohistochemistry. Western blot and wound scratch analyses were used to determine the signaling pathway for CDC5L-mediated activation of CRC growth and migration. Results: We identified CDC5L as a new hTERT promoter-binding protein. Clinically, CDC5L and hTERT expression levels were key factors in the prognosis of CRC patients. CDC5L knockdown inhibited tumor growth by down-regulating hTERT expression, and CDC5L was shown to be a transcriptional activator of hTERT in a luciferase reporter assay. Conclusion: Altogether, the above results demonstrated that CDC5L was positively correlated with hTERT as a key promoter of CRC cells. To some extent, our findings suggest that CDC5L may serve as a novel therapeutic target for human colorectal cancer. |
| first_indexed | 2024-12-13T09:47:20Z |
| format | Article |
| id | doaj.art-4abee2ed1654421780e60febff8b8d2d |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-13T09:47:20Z |
| publishDate | 2017-05-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-4abee2ed1654421780e60febff8b8d2d2022-12-21T23:52:00ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-05-014162475248810.1159/000475916475916CDC5L Promotes hTERT Expression and Colorectal Tumor GrowthJia LiNingning ZhangRui ZhangLongmei SunWendan YuWei GuoYingying GaoMei LiWei LiuPin LiangWuguo DengXiaonan CuiBackground/Aims: Colorectal cancer (CRC) is the third leading cause of cancer-related death worldwide because the survival rate remains low. Cell division cycle 5-like (CDC5L) is highly expressed in some cancer cells, but the mechanism requires clarification. Human telomerase reverse transcriptase (hTERT) plays important roles in CRC. Methods: This study aimed to identify a link between CDC5L and hTERT and to determine their effects on the signaling pathways, migration and prognosis of CRC cells. We first treated LoVo cells with biotin-labeled hTERT and identified CDC5L. Then, pulldown and ChIP assays were used to verify whether CDC5L was a promoter of hTERT. The roles of CDC5L and hTERT in cell growth and migration were studied using siRNA in vivo and in vitro. 130 human CRC specimens were analyzed using immunohistochemistry. Western blot and wound scratch analyses were used to determine the signaling pathway for CDC5L-mediated activation of CRC growth and migration. Results: We identified CDC5L as a new hTERT promoter-binding protein. Clinically, CDC5L and hTERT expression levels were key factors in the prognosis of CRC patients. CDC5L knockdown inhibited tumor growth by down-regulating hTERT expression, and CDC5L was shown to be a transcriptional activator of hTERT in a luciferase reporter assay. Conclusion: Altogether, the above results demonstrated that CDC5L was positively correlated with hTERT as a key promoter of CRC cells. To some extent, our findings suggest that CDC5L may serve as a novel therapeutic target for human colorectal cancer.http://www.karger.com/Article/FullText/475916CDC5LhTERTCRCMigrationPrognosis |
| spellingShingle | Jia Li Ningning Zhang Rui Zhang Longmei Sun Wendan Yu Wei Guo Yingying Gao Mei Li Wei Liu Pin Liang Wuguo Deng Xiaonan Cui CDC5L Promotes hTERT Expression and Colorectal Tumor Growth Cellular Physiology and Biochemistry CDC5L hTERT CRC Migration Prognosis |
| title | CDC5L Promotes hTERT Expression and Colorectal Tumor Growth |
| title_full | CDC5L Promotes hTERT Expression and Colorectal Tumor Growth |
| title_fullStr | CDC5L Promotes hTERT Expression and Colorectal Tumor Growth |
| title_full_unstemmed | CDC5L Promotes hTERT Expression and Colorectal Tumor Growth |
| title_short | CDC5L Promotes hTERT Expression and Colorectal Tumor Growth |
| title_sort | cdc5l promotes htert expression and colorectal tumor growth |
| topic | CDC5L hTERT CRC Migration Prognosis |
| url | http://www.karger.com/Article/FullText/475916 |
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