Botryllin, a Novel Antimicrobial Peptide from the Colonial Ascidian <em>Botryllus schlosseri</em>
By mining the transcriptome of the colonial ascidian <i>Botryllus schlosseri</i>, we identified a transcript for a novel styelin-like antimicrobial peptide, which we named botryllin. The gene is constitutively transcribed by circulating cytotoxic morula cells (MCs) as a pre-propeptide th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/2/74 |
| _version_ | 1797619683901308928 |
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| author | Nicola Franchi Loriano Ballarin Francesca Cima |
| author_facet | Nicola Franchi Loriano Ballarin Francesca Cima |
| author_sort | Nicola Franchi |
| collection | DOAJ |
| description | By mining the transcriptome of the colonial ascidian <i>Botryllus schlosseri</i>, we identified a transcript for a novel styelin-like antimicrobial peptide, which we named botryllin. The gene is constitutively transcribed by circulating cytotoxic morula cells (MCs) as a pre-propeptide that is then cleaved to mature peptide. The synthetic peptide, obtained from in silico translation of the transcript, shows robust killing activity of bacterial and unicellular yeast cells, causing breakages of both the plasma membrane and the cell wall. Specific monoclonal antibodies were raised against the epitopes of the putative amino acid sequence of the propeptide and the mature peptide; in both cases, they label the MC granular content. Upon MC degranulation induced by the presence of nonself, the antibodies recognise the extracellular nets with entrapped bacteria nearby MC remains. The obtained results suggest that the botryllin gene carries the information for the synthesis of an AMP involved in the protection of <i>B. schlosseri</i> from invading foreign cells. |
| first_indexed | 2024-03-11T08:31:21Z |
| format | Article |
| id | doaj.art-4ac75e6bb22d4302ae93f1b29a37fc05 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T08:31:21Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4ac75e6bb22d4302ae93f1b29a37fc052023-11-16T21:47:27ZengMDPI AGMarine Drugs1660-33972023-01-012127410.3390/md21020074Botryllin, a Novel Antimicrobial Peptide from the Colonial Ascidian <em>Botryllus schlosseri</em>Nicola Franchi0Loriano Ballarin1Francesca Cima2Department of Life Science, University of Modena and Reggio Emilia, 41125 Modena, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyBy mining the transcriptome of the colonial ascidian <i>Botryllus schlosseri</i>, we identified a transcript for a novel styelin-like antimicrobial peptide, which we named botryllin. The gene is constitutively transcribed by circulating cytotoxic morula cells (MCs) as a pre-propeptide that is then cleaved to mature peptide. The synthetic peptide, obtained from in silico translation of the transcript, shows robust killing activity of bacterial and unicellular yeast cells, causing breakages of both the plasma membrane and the cell wall. Specific monoclonal antibodies were raised against the epitopes of the putative amino acid sequence of the propeptide and the mature peptide; in both cases, they label the MC granular content. Upon MC degranulation induced by the presence of nonself, the antibodies recognise the extracellular nets with entrapped bacteria nearby MC remains. The obtained results suggest that the botryllin gene carries the information for the synthesis of an AMP involved in the protection of <i>B. schlosseri</i> from invading foreign cells.https://www.mdpi.com/1660-3397/21/2/74antimicrobial peptidesascidians<i>Botryllus</i>haemocytestunicates |
| spellingShingle | Nicola Franchi Loriano Ballarin Francesca Cima Botryllin, a Novel Antimicrobial Peptide from the Colonial Ascidian <em>Botryllus schlosseri</em> Marine Drugs antimicrobial peptides ascidians <i>Botryllus</i> haemocytes tunicates |
| title | Botryllin, a Novel Antimicrobial Peptide from the Colonial Ascidian <em>Botryllus schlosseri</em> |
| title_full | Botryllin, a Novel Antimicrobial Peptide from the Colonial Ascidian <em>Botryllus schlosseri</em> |
| title_fullStr | Botryllin, a Novel Antimicrobial Peptide from the Colonial Ascidian <em>Botryllus schlosseri</em> |
| title_full_unstemmed | Botryllin, a Novel Antimicrobial Peptide from the Colonial Ascidian <em>Botryllus schlosseri</em> |
| title_short | Botryllin, a Novel Antimicrobial Peptide from the Colonial Ascidian <em>Botryllus schlosseri</em> |
| title_sort | botryllin a novel antimicrobial peptide from the colonial ascidian em botryllus schlosseri em |
| topic | antimicrobial peptides ascidians <i>Botryllus</i> haemocytes tunicates |
| url | https://www.mdpi.com/1660-3397/21/2/74 |
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