Characterization and pathogenicity of <em>Plectosphaerella</em> spp. collected from basil and parsley in Italy
From 2012–2016 plants of basil (Ocimum basilicum L.) and parsley (Petroselinum sativum Hoffm.) showing decline symptoms were collected from local markets in Foggia Province (southern Italy) and assessed to determine their main fungal pathogens. These plants showed symptoms including leaf yellowing,...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Firenze University Press
2018-09-01
|
| Series: | Phytopathologia Mediterranea |
| Subjects: | |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5767 |
| _version_ | 1830442645653028864 |
|---|---|
| author | Maria RAIMONDO Antonia CARLUCCI |
| author_facet | Maria RAIMONDO Antonia CARLUCCI |
| author_sort | Maria RAIMONDO |
| collection | DOAJ |
| description | From 2012–2016 plants of basil (Ocimum basilicum L.) and parsley (Petroselinum sativum Hoffm.) showing decline symptoms were collected from local markets in Foggia Province (southern Italy) and assessed to determine their main fungal pathogens. These plants showed symptoms including leaf yellowing, necrotic lesions on stems, collar and roots, and in some cases, stunting of entire plants. Mycological analyses revealed fungal isolates mainly belonging to the Plectosphaerella genus. Molecular and morphological studies identified four species of Plectosphaerella: Plectosphaerella cucumerina, P. pauciseptata, P. plurivora, and P. ramiseptata. To understand the pathogenic roles of these fungi, and five other reference Plectosphaerella spp., pathogenicity tests were performed in vitro and in-vivo using, respectively, detached leaves and 30-d-old plants of basil (cv. Napoletano) and parsley (cv. Gigante di Napoli). All the fungal species isolated produced host symptoms, including necrotic spots, parenchymatic patches, hydropic areas, collar and root discolouration on leaves and young plants, with varying severity. The most aggressive species on both plants were P. pauciseptata and P. ramiseptata, while P. alismatis, P. citrulli, P. cucumerina, P. delsorboi, P. melonis, and P. plurivora gave less disease severity on both plants. This is the first report worldwide of P. cucumerina, P. pauciseptata, and P. ramiseptata as pathogens of parsley, and P. pauciseptata, P. plurivora, and P. ramiseptata as pathogens of basil. |
| first_indexed | 2024-12-21T05:42:25Z |
| format | Article |
| id | doaj.art-4acc5a225847463dadc2a3508a5d6e32 |
| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-12-21T05:42:25Z |
| publishDate | 2018-09-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj.art-4acc5a225847463dadc2a3508a5d6e322022-12-21T19:14:13ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952018-09-0157210.14601/Phytopathol_Mediterr-23206Characterization and pathogenicity of <em>Plectosphaerella</em> spp. collected from basil and parsley in ItalyMaria RAIMONDO0Antonia CARLUCCI1Department of Sciences of Agriculture, Food and the Environment, University of Foggia, Foggia, ItalyDepartment of Sciences of Agriculture, Food and the Environment, University of Foggia, Foggia, ItalyFrom 2012–2016 plants of basil (Ocimum basilicum L.) and parsley (Petroselinum sativum Hoffm.) showing decline symptoms were collected from local markets in Foggia Province (southern Italy) and assessed to determine their main fungal pathogens. These plants showed symptoms including leaf yellowing, necrotic lesions on stems, collar and roots, and in some cases, stunting of entire plants. Mycological analyses revealed fungal isolates mainly belonging to the Plectosphaerella genus. Molecular and morphological studies identified four species of Plectosphaerella: Plectosphaerella cucumerina, P. pauciseptata, P. plurivora, and P. ramiseptata. To understand the pathogenic roles of these fungi, and five other reference Plectosphaerella spp., pathogenicity tests were performed in vitro and in-vivo using, respectively, detached leaves and 30-d-old plants of basil (cv. Napoletano) and parsley (cv. Gigante di Napoli). All the fungal species isolated produced host symptoms, including necrotic spots, parenchymatic patches, hydropic areas, collar and root discolouration on leaves and young plants, with varying severity. The most aggressive species on both plants were P. pauciseptata and P. ramiseptata, while P. alismatis, P. citrulli, P. cucumerina, P. delsorboi, P. melonis, and P. plurivora gave less disease severity on both plants. This is the first report worldwide of P. cucumerina, P. pauciseptata, and P. ramiseptata as pathogens of parsley, and P. pauciseptata, P. plurivora, and P. ramiseptata as pathogens of basil.https://oajournals.fupress.net/index.php/pm/article/view/5767Ocimum basilicumPetroselinum sativumendophyteshemibiotrophs |
| spellingShingle | Maria RAIMONDO Antonia CARLUCCI Characterization and pathogenicity of <em>Plectosphaerella</em> spp. collected from basil and parsley in Italy Phytopathologia Mediterranea Ocimum basilicum Petroselinum sativum endophytes hemibiotrophs |
| title | Characterization and pathogenicity of <em>Plectosphaerella</em> spp. collected from basil and parsley in Italy |
| title_full | Characterization and pathogenicity of <em>Plectosphaerella</em> spp. collected from basil and parsley in Italy |
| title_fullStr | Characterization and pathogenicity of <em>Plectosphaerella</em> spp. collected from basil and parsley in Italy |
| title_full_unstemmed | Characterization and pathogenicity of <em>Plectosphaerella</em> spp. collected from basil and parsley in Italy |
| title_short | Characterization and pathogenicity of <em>Plectosphaerella</em> spp. collected from basil and parsley in Italy |
| title_sort | characterization and pathogenicity of em plectosphaerella em spp collected from basil and parsley in italy |
| topic | Ocimum basilicum Petroselinum sativum endophytes hemibiotrophs |
| url | https://oajournals.fupress.net/index.php/pm/article/view/5767 |
| work_keys_str_mv | AT mariaraimondo characterizationandpathogenicityofemplectosphaerellaemsppcollectedfrombasilandparsleyinitaly AT antoniacarlucci characterizationandpathogenicityofemplectosphaerellaemsppcollectedfrombasilandparsleyinitaly |