Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis
Summary: Timely diagnosis of Schistosoma infection, particularly in the early stage is crucial for identifying infected hosts and then taking effective control strategies. Here, metagenomic next-generation sequencing was used to identify pathogen-specific circulating DNAs (cDNAs) in the sera/plasma...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223015729 |
| _version_ | 1797742168219058176 |
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| author | Jingyi Liu Xiaoxu Wang Fei Sheng Bikash R. Giri Shun Li Tianqi Xia Xuxin Li Guofeng Cheng |
| author_facet | Jingyi Liu Xiaoxu Wang Fei Sheng Bikash R. Giri Shun Li Tianqi Xia Xuxin Li Guofeng Cheng |
| author_sort | Jingyi Liu |
| collection | DOAJ |
| description | Summary: Timely diagnosis of Schistosoma infection, particularly in the early stage is crucial for identifying infected hosts and then taking effective control strategies. Here, metagenomic next-generation sequencing was used to identify pathogen-specific circulating DNAs (cDNAs) in the sera/plasma of New Zealand rabbits infected with S. japonicum, and the identified cDNAs were validated by PCR and qPCR. Loop-mediated isothermal amplification (LAMP)-based CRISPR-Cas12a and recombinase polymerase amplification-based lateral flow strip (RPA-LF) methods combined with the newly identified cDNA were developed to evaluate the potentials for diagnosing murine and human schistosomiasis. The results indicated that twenty-two cDNAs were identified. The developed LAMP-based CRISPR/Cas12a and RPA-LF methods showed a good potential for diagnosing murine or human schistosomiasis as early as 5 days of post-infection with 5 cercariae infection. In a word, S. japonicum specific cDNAs in circulation of infected hosts could be effective biomarkers for detecting Schistosoma infection particularly for early stages. |
| first_indexed | 2024-03-12T14:37:07Z |
| format | Article |
| id | doaj.art-4accb7a509ee49e99809b973a7a05869 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T14:37:07Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-4accb7a509ee49e99809b973a7a058692023-08-17T04:27:50ZengElsevieriScience2589-00422023-09-01269107495Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasisJingyi Liu0Xiaoxu Wang1Fei Sheng2Bikash R. Giri3Shun Li4Tianqi Xia5Xuxin Li6Guofeng Cheng7Shanghai Tenth People’s Hospital, Tongji University School of Medicine, #500 Zhen-nan Road, Shanghai 200331, People’s Republic of China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, People’s Republic of ChinaSchool of Biotechnology Jiangsu University of Science and Technology, Zhen Jiang 212100, People’s Republic of ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, #500 Zhen-nan Road, Shanghai 200331, People’s Republic of ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, #500 Zhen-nan Road, Shanghai 200331, People’s Republic of ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, People’s Republic of ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, People’s Republic of ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, People’s Republic of ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, #500 Zhen-nan Road, Shanghai 200331, People’s Republic of China; Corresponding authorSummary: Timely diagnosis of Schistosoma infection, particularly in the early stage is crucial for identifying infected hosts and then taking effective control strategies. Here, metagenomic next-generation sequencing was used to identify pathogen-specific circulating DNAs (cDNAs) in the sera/plasma of New Zealand rabbits infected with S. japonicum, and the identified cDNAs were validated by PCR and qPCR. Loop-mediated isothermal amplification (LAMP)-based CRISPR-Cas12a and recombinase polymerase amplification-based lateral flow strip (RPA-LF) methods combined with the newly identified cDNA were developed to evaluate the potentials for diagnosing murine and human schistosomiasis. The results indicated that twenty-two cDNAs were identified. The developed LAMP-based CRISPR/Cas12a and RPA-LF methods showed a good potential for diagnosing murine or human schistosomiasis as early as 5 days of post-infection with 5 cercariae infection. In a word, S. japonicum specific cDNAs in circulation of infected hosts could be effective biomarkers for detecting Schistosoma infection particularly for early stages.http://www.sciencedirect.com/science/article/pii/S2589004223015729Biochemical assayGenomicsMolecular Genetics |
| spellingShingle | Jingyi Liu Xiaoxu Wang Fei Sheng Bikash R. Giri Shun Li Tianqi Xia Xuxin Li Guofeng Cheng Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis iScience Biochemical assay Genomics Molecular Genetics |
| title | Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis |
| title_full | Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis |
| title_fullStr | Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis |
| title_full_unstemmed | Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis |
| title_short | Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis |
| title_sort | metagenomic sequencing for identifying pathogen specific circulating dnas and development of diagnostic methods for schistosomiasis |
| topic | Biochemical assay Genomics Molecular Genetics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223015729 |
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