Circulating maternal chimeric cells have an impact on the outcome of biliary atresia
IntroductionWe aimed to quantify the DNA of maternal chimeric (MC) cells in the peripheral blood of the BA patients and investigated the impact on the outcome.MethodsPatients with progressive jaundice because of no bile flow, which necessitated liver transplantation, or who showed inadequate bile fl...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Pediatrics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fped.2022.1007927/full |
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| author | Ryuta Masuya Ryuta Masuya Toshihiro Muraji Sami B. Kanaan Sami B. Kanaan Toshio Harumatsu Mitsuru Muto Miki Toma Toshihiro Yanai Anne M. Stevens J. Lee Nelson Kazuhiko Nakame Kazuhiko Nakame Atsushi Nanashima Satoshi Ieiri |
| author_facet | Ryuta Masuya Ryuta Masuya Toshihiro Muraji Sami B. Kanaan Sami B. Kanaan Toshio Harumatsu Mitsuru Muto Miki Toma Toshihiro Yanai Anne M. Stevens J. Lee Nelson Kazuhiko Nakame Kazuhiko Nakame Atsushi Nanashima Satoshi Ieiri |
| author_sort | Ryuta Masuya |
| collection | DOAJ |
| description | IntroductionWe aimed to quantify the DNA of maternal chimeric (MC) cells in the peripheral blood of the BA patients and investigated the impact on the outcome.MethodsPatients with progressive jaundice because of no bile flow, which necessitated liver transplantation, or who showed inadequate bile flow with or without episodes of cholangitis and progressive hepatic fibrosis and portal hypertension were classified into the poor group. Those with adequate bile flow with completely normal liver function tests beyond 2 years were classified into the good group. The qPCR were separately carried out in buffy coat samples and plasma samples, targeting the non-inherited maternal HLA alleles in the DNA samples.ResultsMC-DNA was present in the buffy coat (10–328 gEq per 106 host cells) in seven patients. There was no MC-DNA in the remaining five patients. MC-DNA (214–15,331 gEq per 106 host cells) was observed in the plasma of five patients. The quantity of MC-DNA in the buffy coat showed a significant difference between the two prognostic groups (p = 0.018), whereas there was no significant difference in the quantity of MC-DNA in plasma (p = 0.205). MC-DNA in the buffy coat was significantly associated with the outcome (p = 0.028), whereas MC-DNA in the plasma did not influence the outcome (p = 0.56).ConclusionsPoor outcomes in BA were correlated with circulating maternal chimeric lymphocytes. |
| first_indexed | 2024-04-14T08:18:14Z |
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| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-04-14T08:18:14Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-4accbfb34b574ce1bc976942b21960da2022-12-22T02:04:19ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602022-09-011010.3389/fped.2022.10079271007927Circulating maternal chimeric cells have an impact on the outcome of biliary atresiaRyuta Masuya0Ryuta Masuya1Toshihiro Muraji2Sami B. Kanaan3Sami B. Kanaan4Toshio Harumatsu5Mitsuru Muto6Miki Toma7Toshihiro Yanai8Anne M. Stevens9J. Lee Nelson10Kazuhiko Nakame11Kazuhiko Nakame12Atsushi Nanashima13Satoshi Ieiri14Division of the Gastrointestinal, Endocrine and Pediatric Surgery, Department of Surgery, Faculty of Medicine, University of Miyazaki, Miyazaki, JapanDepartment of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Kagoshima, JapanDepartment of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Kagoshima, JapanResearch and Development, Chimerocyte, Inc., Seattle, WA, United StatesClinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United StatesDepartment of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Kagoshima, JapanDepartment of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Kagoshima, JapanDepartment of Pediatric Surgery, Ibaraki Children's Hospital, Mito, JapanDepartment of Pediatric Surgery, Ibaraki Children's Hospital, Mito, JapanSeattle Children's Research Institute, University of Washington, Seattle, WA, United StatesClinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United StatesDivision of the Gastrointestinal, Endocrine and Pediatric Surgery, Department of Surgery, Faculty of Medicine, University of Miyazaki, Miyazaki, JapanDepartment of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Kagoshima, JapanDivision of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, Faculty of Medicine, University of Miyazaki, Miyazaki, JapanDepartment of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Kagoshima, JapanIntroductionWe aimed to quantify the DNA of maternal chimeric (MC) cells in the peripheral blood of the BA patients and investigated the impact on the outcome.MethodsPatients with progressive jaundice because of no bile flow, which necessitated liver transplantation, or who showed inadequate bile flow with or without episodes of cholangitis and progressive hepatic fibrosis and portal hypertension were classified into the poor group. Those with adequate bile flow with completely normal liver function tests beyond 2 years were classified into the good group. The qPCR were separately carried out in buffy coat samples and plasma samples, targeting the non-inherited maternal HLA alleles in the DNA samples.ResultsMC-DNA was present in the buffy coat (10–328 gEq per 106 host cells) in seven patients. There was no MC-DNA in the remaining five patients. MC-DNA (214–15,331 gEq per 106 host cells) was observed in the plasma of five patients. The quantity of MC-DNA in the buffy coat showed a significant difference between the two prognostic groups (p = 0.018), whereas there was no significant difference in the quantity of MC-DNA in plasma (p = 0.205). MC-DNA in the buffy coat was significantly associated with the outcome (p = 0.028), whereas MC-DNA in the plasma did not influence the outcome (p = 0.56).ConclusionsPoor outcomes in BA were correlated with circulating maternal chimeric lymphocytes.https://www.frontiersin.org/articles/10.3389/fped.2022.1007927/fullbiliary atresiamaternal microchimerismqPCRprognosisGVHDautoimmunity |
| spellingShingle | Ryuta Masuya Ryuta Masuya Toshihiro Muraji Sami B. Kanaan Sami B. Kanaan Toshio Harumatsu Mitsuru Muto Miki Toma Toshihiro Yanai Anne M. Stevens J. Lee Nelson Kazuhiko Nakame Kazuhiko Nakame Atsushi Nanashima Satoshi Ieiri Circulating maternal chimeric cells have an impact on the outcome of biliary atresia Frontiers in Pediatrics biliary atresia maternal microchimerism qPCR prognosis GVHD autoimmunity |
| title | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
| title_full | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
| title_fullStr | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
| title_full_unstemmed | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
| title_short | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
| title_sort | circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
| topic | biliary atresia maternal microchimerism qPCR prognosis GVHD autoimmunity |
| url | https://www.frontiersin.org/articles/10.3389/fped.2022.1007927/full |
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