Mammalian Cell Surface Display as a Novel Method for Developing Engineered Lectins with Novel Characteristics

Mammalian Cell Surface Display as a Novel Method for Developing Engineered Lectins with Novel Characteristics

Leguminous lectins have a conserved carbohydrate recognition site comprising four loops (A–D). Here, we randomly mutated the sequence and length of loops C and D of peanut agglutinin (PNA) and expressed the proteins on the surface of mouse green fluorescent protein (GFP)-reporter cells. Flow cytomet...

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Bibliographic Details
Main Authors: Keisuke Soga, Hirohito Abo, Sheng-Ying Qin, Takuya Kyoutou, Keiko Hiemori, Hiroaki Tateno, Naoki Matsumoto, Jun Hirabayashi, Kazuo Yamamoto
Format: Article
Language:English
Published: MDPI AG 2015-07-01
Series:Biomolecules
Subjects:
leguminous lectin
cell surface display
carbohydrate-binding specificity
molecular engineering
scaffold
Online Access:http://www.mdpi.com/2218-273X/5/3/1540

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