Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, <i>Escherichia coli</i> Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania
We determined the phenotypic profile of multidrug-resistant (MDR) <i>Escherichia coli</i> isolated from 698 samples (390 and 308 from poultry and domestic pigs, respectively). In total, 562 <i>Enterobacteria</i> were isolated. About 80.5% of the isolates were <i>E. coli...
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MDPI AG
2021-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/4/406 |
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| author | Zuhura I. Kimera Fauster X. Mgaya Gerald Misinzo Stephen E. Mshana Nyambura Moremi Mecky I. N. Matee |
| author_facet | Zuhura I. Kimera Fauster X. Mgaya Gerald Misinzo Stephen E. Mshana Nyambura Moremi Mecky I. N. Matee |
| author_sort | Zuhura I. Kimera |
| collection | DOAJ |
| description | We determined the phenotypic profile of multidrug-resistant (MDR) <i>Escherichia coli</i> isolated from 698 samples (390 and 308 from poultry and domestic pigs, respectively). In total, 562 <i>Enterobacteria</i> were isolated. About 80.5% of the isolates were <i>E. coli</i>. Occurrence of <i>E. coli</i> was significantly higher among domestic pigs (73.1%) than in poultry (60.5%) (<i>p</i> = 0.000). In both poultry and domestic pigs, <i>E. coli</i> isolates were highly resistant to tetracycline (63.5%), nalidixic acid (53.7%), ampicillin (52.3%), and trimethoprim/sulfamethoxazole (50.9%). About 51.6%, 65.3%, and 53.7% of <i>E. coli</i> were MDR, extended-spectrum beta lactamase-producing <i>enterobacteriaceae</i> (ESBL-PE), and quinolone-resistant, respectively. A total of 68% of the extended-spectrum beta lactamase (ESBL) producers were also resistant to quinolones. For all tested antibiotics, resistance was significantly higher in ESBL-producing and quinolone-resistant isolates than the non-ESBL producers and non-quinolone-resistant <i>E. coli</i>. Eight isolates were resistant to eight classes of antimicrobials. We compared phenotypic with genotypic results of 20 MDR <i>E. coli</i> isolates, ESBL producers, and quinolone-resistant strains and found 80% harbored <i>bla</i>CTX-M, 15% <i>aac</i>(<i>6</i>)-<i>lb</i>-<i>cr</i>, 10% <i>qnrB</i>, and 5% <i>qepA</i>. None harbored TEM, SHV, <i>qnrA</i>, <i>qnrS</i>, <i>qnrC</i>, or <i>qnrD</i>. The observed pattern and level of resistance render this portfolio of antibiotics ineffective for their intended use. |
| first_indexed | 2024-03-10T12:28:53Z |
| format | Article |
| id | doaj.art-4ad4741a6512449e9bea2682fe187a8a |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T12:28:53Z |
| publishDate | 2021-04-01 |
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| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-4ad4741a6512449e9bea2682fe187a8a2023-11-21T14:46:53ZengMDPI AGAntibiotics2079-63822021-04-0110440610.3390/antibiotics10040406Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, <i>Escherichia coli</i> Isolated from Poultry and Domestic Pigs in Dar es Salaam, TanzaniaZuhura I. Kimera0Fauster X. Mgaya1Gerald Misinzo2Stephen E. Mshana3Nyambura Moremi4Mecky I. N. Matee5Department of Microbiology and Immunology, School of Medicine, Muhimbili University of Health and Allied Sciences, Dar es Salaam 11103, TanzaniaDepartment of Microbiology and Immunology, School of Medicine, Muhimbili University of Health and Allied Sciences, Dar es Salaam 11103, TanzaniaSACIDS Africa Centre of Excellence for Infectious Diseases, Sokoine University of Agriculture, Morogoro 67125, TanzaniaDepartment of Microbiology and Immunology, School of Medicine, Catholic University of Health and Allied Sciences, Mwanza 33109, TanzaniaMinistry of Health, Community Development, Gender, Elderly and Children, Dar es Salaam 11101, TanzaniaDepartment of Microbiology and Immunology, School of Medicine, Muhimbili University of Health and Allied Sciences, Dar es Salaam 11103, TanzaniaWe determined the phenotypic profile of multidrug-resistant (MDR) <i>Escherichia coli</i> isolated from 698 samples (390 and 308 from poultry and domestic pigs, respectively). In total, 562 <i>Enterobacteria</i> were isolated. About 80.5% of the isolates were <i>E. coli</i>. Occurrence of <i>E. coli</i> was significantly higher among domestic pigs (73.1%) than in poultry (60.5%) (<i>p</i> = 0.000). In both poultry and domestic pigs, <i>E. coli</i> isolates were highly resistant to tetracycline (63.5%), nalidixic acid (53.7%), ampicillin (52.3%), and trimethoprim/sulfamethoxazole (50.9%). About 51.6%, 65.3%, and 53.7% of <i>E. coli</i> were MDR, extended-spectrum beta lactamase-producing <i>enterobacteriaceae</i> (ESBL-PE), and quinolone-resistant, respectively. A total of 68% of the extended-spectrum beta lactamase (ESBL) producers were also resistant to quinolones. For all tested antibiotics, resistance was significantly higher in ESBL-producing and quinolone-resistant isolates than the non-ESBL producers and non-quinolone-resistant <i>E. coli</i>. Eight isolates were resistant to eight classes of antimicrobials. We compared phenotypic with genotypic results of 20 MDR <i>E. coli</i> isolates, ESBL producers, and quinolone-resistant strains and found 80% harbored <i>bla</i>CTX-M, 15% <i>aac</i>(<i>6</i>)-<i>lb</i>-<i>cr</i>, 10% <i>qnrB</i>, and 5% <i>qepA</i>. None harbored TEM, SHV, <i>qnrA</i>, <i>qnrS</i>, <i>qnrC</i>, or <i>qnrD</i>. The observed pattern and level of resistance render this portfolio of antibiotics ineffective for their intended use.https://www.mdpi.com/2079-6382/10/4/406poultrydomestic pigsantibioticsantimicrobial resistanceMsimbazi basinfarmers |
| spellingShingle | Zuhura I. Kimera Fauster X. Mgaya Gerald Misinzo Stephen E. Mshana Nyambura Moremi Mecky I. N. Matee Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, <i>Escherichia coli</i> Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania Antibiotics poultry domestic pigs antibiotics antimicrobial resistance Msimbazi basin farmers |
| title | Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, <i>Escherichia coli</i> Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania |
| title_full | Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, <i>Escherichia coli</i> Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania |
| title_fullStr | Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, <i>Escherichia coli</i> Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania |
| title_full_unstemmed | Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, <i>Escherichia coli</i> Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania |
| title_short | Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, <i>Escherichia coli</i> Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania |
| title_sort | multidrug resistant including extended spectrum beta lactamase producing and quinolone resistant i escherichia coli i isolated from poultry and domestic pigs in dar es salaam tanzania |
| topic | poultry domestic pigs antibiotics antimicrobial resistance Msimbazi basin farmers |
| url | https://www.mdpi.com/2079-6382/10/4/406 |
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