Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight
This study investigated the effect of prenatal nutrition on liver metabolome and on body (BW) and liver weight (LW) of Nellore bulls at slaughter. Three treatments were applied in 126 cows during pregnancy: NP—control (mineral supplementation); PP—protein-energy supplementation in the third trimeste...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/5/441 |
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| author | Guilherme Henrique Gebim Polizel Fernando Augusto Correia Queiroz Cançado Evandro Fernando Ferreira Dias Arícia Christofaro Fernandes Roberta Cavalcante Cracco Brenda Tonsic Carmona Henrique Hespanhol Castellar Mirele Daiana Poleti Miguel Henrique de Almeida Santana |
| author_facet | Guilherme Henrique Gebim Polizel Fernando Augusto Correia Queiroz Cançado Evandro Fernando Ferreira Dias Arícia Christofaro Fernandes Roberta Cavalcante Cracco Brenda Tonsic Carmona Henrique Hespanhol Castellar Mirele Daiana Poleti Miguel Henrique de Almeida Santana |
| author_sort | Guilherme Henrique Gebim Polizel |
| collection | DOAJ |
| description | This study investigated the effect of prenatal nutrition on liver metabolome and on body (BW) and liver weight (LW) of Nellore bulls at slaughter. Three treatments were applied in 126 cows during pregnancy: NP—control (mineral supplementation); PP—protein-energy supplementation in the third trimester; and FP—protein-energy supplementation during the entire pregnancy. Offspring BW and LW were evaluated, and a targeted metabolomics analysis was performed on their livers (<i>n</i> = 18, 22.5 ± 1 months of age). Data were submitted to principal component analysis (PCA), analysis of variance (ANOVA), enrichment analysis, and Pearson’s correlation analysis. The phenotypes did not show differences between treatments (<i>p</i> > 0.05). Metabolites PCA showed an overlap of treatment clusters in the analysis. We found significant metabolites in ANOVA (<i>p</i> ≤ 0.05; Glycine, Hydroxytetradecadienylcarnitine, Aminoadipic acid and Carnosine). Enrichment analysis revealed some biological processes (Histidine metabolism, beta-Alanine metabolism, and Lysine degradation). Pearson’s correlation analysis showed 29 significant correlated metabolites with BW and 1 metabolite correlated with LW. In summary, prenatal nutrition did not show effects on the phenotypes evaluated, but affected some metabolites and biological pathways, mainly related to oxidative metabolism. In addition, BW seems to influence the hepatic metabolome more than LW, due to the amount and magnitude of correlations found. |
| first_indexed | 2024-03-10T03:25:55Z |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T03:25:55Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-4ad50f83a11b4b7eb7ea638985296f172023-11-23T12:07:30ZengMDPI AGMetabolites2218-19892022-05-0112544110.3390/metabo12050441Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver WeightGuilherme Henrique Gebim Polizel0Fernando Augusto Correia Queiroz Cançado1Evandro Fernando Ferreira Dias2Arícia Christofaro Fernandes3Roberta Cavalcante Cracco4Brenda Tonsic Carmona5Henrique Hespanhol Castellar6Mirele Daiana Poleti7Miguel Henrique de Almeida Santana8Department of Animal Science, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Basic Sciences, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Basic Sciences, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering–USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilThis study investigated the effect of prenatal nutrition on liver metabolome and on body (BW) and liver weight (LW) of Nellore bulls at slaughter. Three treatments were applied in 126 cows during pregnancy: NP—control (mineral supplementation); PP—protein-energy supplementation in the third trimester; and FP—protein-energy supplementation during the entire pregnancy. Offspring BW and LW were evaluated, and a targeted metabolomics analysis was performed on their livers (<i>n</i> = 18, 22.5 ± 1 months of age). Data were submitted to principal component analysis (PCA), analysis of variance (ANOVA), enrichment analysis, and Pearson’s correlation analysis. The phenotypes did not show differences between treatments (<i>p</i> > 0.05). Metabolites PCA showed an overlap of treatment clusters in the analysis. We found significant metabolites in ANOVA (<i>p</i> ≤ 0.05; Glycine, Hydroxytetradecadienylcarnitine, Aminoadipic acid and Carnosine). Enrichment analysis revealed some biological processes (Histidine metabolism, beta-Alanine metabolism, and Lysine degradation). Pearson’s correlation analysis showed 29 significant correlated metabolites with BW and 1 metabolite correlated with LW. In summary, prenatal nutrition did not show effects on the phenotypes evaluated, but affected some metabolites and biological pathways, mainly related to oxidative metabolism. In addition, BW seems to influence the hepatic metabolome more than LW, due to the amount and magnitude of correlations found.https://www.mdpi.com/2218-1989/12/5/441beef cattlefetal programminghepatic metabolitesmaternal nutrition |
| spellingShingle | Guilherme Henrique Gebim Polizel Fernando Augusto Correia Queiroz Cançado Evandro Fernando Ferreira Dias Arícia Christofaro Fernandes Roberta Cavalcante Cracco Brenda Tonsic Carmona Henrique Hespanhol Castellar Mirele Daiana Poleti Miguel Henrique de Almeida Santana Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight Metabolites beef cattle fetal programming hepatic metabolites maternal nutrition |
| title | Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight |
| title_full | Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight |
| title_fullStr | Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight |
| title_full_unstemmed | Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight |
| title_short | Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight |
| title_sort | effects of different prenatal nutrition strategies on the liver metabolome of bulls and its correlation with body and liver weight |
| topic | beef cattle fetal programming hepatic metabolites maternal nutrition |
| url | https://www.mdpi.com/2218-1989/12/5/441 |
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