Serum Anti-Müllerian Hormone Levels and Estrous Monitoring of GnRH Agonist Deslorelin-Induced Estrus in Bitches: A Pilot Study
This study was performed to monitor estrous patterns and, more importantly, changes in anti-Müllerian hormone (AMH) concentrations during the peri-ovulatory period in deslorelin-induced estrous bitches. Healthy anestrous bitches (<i>n</i> = 4) were used. Estrus and ovulation were monitor...
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MDPI AG
2023-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/2/258 |
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| author | Sroisuda Chotimanukul Sandra Goericke-Pesch Junpen Suwimonteerabutr Jinda Singlor Ekkaphot Sangkrachang Padet Tummaruk Suppawiwat Ponglowhapan |
| author_facet | Sroisuda Chotimanukul Sandra Goericke-Pesch Junpen Suwimonteerabutr Jinda Singlor Ekkaphot Sangkrachang Padet Tummaruk Suppawiwat Ponglowhapan |
| author_sort | Sroisuda Chotimanukul |
| collection | DOAJ |
| description | This study was performed to monitor estrous patterns and, more importantly, changes in anti-Müllerian hormone (AMH) concentrations during the peri-ovulatory period in deslorelin-induced estrous bitches. Healthy anestrous bitches (<i>n</i> = 4) were used. Estrus and ovulation were monitored after deslorelin implantation. Blood samples were collected for analysis of progesterone, estradiol-17ß and AMH concentrations before implantation (day 0) and on days 6, 8, 10, 12, 14, 16, 18, 20 and 22 after implantation. Six days following treatment, all bitches showed estrus signs. Ovulation took place between days 12 and 15. Circulating AMH concentrations varied among bitches from 0.12 to 3.08 ng/mL. However, no significant differences in AMH levels (mean ± SD) were observed between day 0 and days following post-implantation <i>(p ></i> 0.05). There were no significant correlations between AMH and estradiol or AMH and progesterone <i>(p ></i> 0.05). Ultrasonographically, the number of clearly identifiable ovarian follicles was higher before ovulation and the area of ovaries increased after ovulation <i>(p <</i> 0.05). Except for AMH, changes in vaginal cytology, estradiol-17ß and progesterone levels observed in our study were similar to naturally occurring estrus. Large intra- and inter-individual variation in AMH were observed suggesting that AMH is currently not suitable as a canine fertility marker to monitor ovarian response to deslorelin treatment for estrus induction. |
| first_indexed | 2024-03-09T13:48:58Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T13:48:58Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4ad7035058004ceabd1d4f807677fc402023-11-30T20:53:24ZengMDPI AGAnimals2076-26152023-01-0113225810.3390/ani13020258Serum Anti-Müllerian Hormone Levels and Estrous Monitoring of GnRH Agonist Deslorelin-Induced Estrus in Bitches: A Pilot StudySroisuda Chotimanukul0Sandra Goericke-Pesch1Junpen Suwimonteerabutr2Jinda Singlor3Ekkaphot Sangkrachang4Padet Tummaruk5Suppawiwat Ponglowhapan6Department of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandReproductive Unit, Clinic for Small Animals, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyDepartment of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandThis study was performed to monitor estrous patterns and, more importantly, changes in anti-Müllerian hormone (AMH) concentrations during the peri-ovulatory period in deslorelin-induced estrous bitches. Healthy anestrous bitches (<i>n</i> = 4) were used. Estrus and ovulation were monitored after deslorelin implantation. Blood samples were collected for analysis of progesterone, estradiol-17ß and AMH concentrations before implantation (day 0) and on days 6, 8, 10, 12, 14, 16, 18, 20 and 22 after implantation. Six days following treatment, all bitches showed estrus signs. Ovulation took place between days 12 and 15. Circulating AMH concentrations varied among bitches from 0.12 to 3.08 ng/mL. However, no significant differences in AMH levels (mean ± SD) were observed between day 0 and days following post-implantation <i>(p ></i> 0.05). There were no significant correlations between AMH and estradiol or AMH and progesterone <i>(p ></i> 0.05). Ultrasonographically, the number of clearly identifiable ovarian follicles was higher before ovulation and the area of ovaries increased after ovulation <i>(p <</i> 0.05). Except for AMH, changes in vaginal cytology, estradiol-17ß and progesterone levels observed in our study were similar to naturally occurring estrus. Large intra- and inter-individual variation in AMH were observed suggesting that AMH is currently not suitable as a canine fertility marker to monitor ovarian response to deslorelin treatment for estrus induction.https://www.mdpi.com/2076-2615/13/2/258AMHdeslorelindogestrus inductionGnRH agonist |
| spellingShingle | Sroisuda Chotimanukul Sandra Goericke-Pesch Junpen Suwimonteerabutr Jinda Singlor Ekkaphot Sangkrachang Padet Tummaruk Suppawiwat Ponglowhapan Serum Anti-Müllerian Hormone Levels and Estrous Monitoring of GnRH Agonist Deslorelin-Induced Estrus in Bitches: A Pilot Study Animals AMH deslorelin dog estrus induction GnRH agonist |
| title | Serum Anti-Müllerian Hormone Levels and Estrous Monitoring of GnRH Agonist Deslorelin-Induced Estrus in Bitches: A Pilot Study |
| title_full | Serum Anti-Müllerian Hormone Levels and Estrous Monitoring of GnRH Agonist Deslorelin-Induced Estrus in Bitches: A Pilot Study |
| title_fullStr | Serum Anti-Müllerian Hormone Levels and Estrous Monitoring of GnRH Agonist Deslorelin-Induced Estrus in Bitches: A Pilot Study |
| title_full_unstemmed | Serum Anti-Müllerian Hormone Levels and Estrous Monitoring of GnRH Agonist Deslorelin-Induced Estrus in Bitches: A Pilot Study |
| title_short | Serum Anti-Müllerian Hormone Levels and Estrous Monitoring of GnRH Agonist Deslorelin-Induced Estrus in Bitches: A Pilot Study |
| title_sort | serum anti mullerian hormone levels and estrous monitoring of gnrh agonist deslorelin induced estrus in bitches a pilot study |
| topic | AMH deslorelin dog estrus induction GnRH agonist |
| url | https://www.mdpi.com/2076-2615/13/2/258 |
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