KRT23 promotes the proliferation of liver cancer by activating AKT signaling pathway
Objective To investigate the effect of high keratin 23 （KRT23） expression promoting cell proliferation on liver cancer. Methods The expression of KRT23 in liver cancer was analyzed by bioinformatics. After overexpression and inhibition of KRT23， the effect of KRT23 on the proliferation of liver can...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of New Medicine
2024-03-01
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| Series: | Xin yixue |
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| Online Access: | https://www.xinyixue.cn/fileup/0253-9802/PDF/1712537348253-1480554338.pdf |
| _version_ | 1797218168833310720 |
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| author | Liang Xinghua, Liu Foqiu, Yan Rong, Hu Juan |
| author_facet | Liang Xinghua, Liu Foqiu, Yan Rong, Hu Juan |
| author_sort | Liang Xinghua, Liu Foqiu, Yan Rong, Hu Juan |
| collection | DOAJ |
| description | Objective To investigate the effect of high keratin 23 （KRT23） expression promoting cell proliferation on liver cancer. Methods The expression of KRT23 in liver cancer was analyzed by bioinformatics. After overexpression and inhibition of KRT23， the effect of KRT23 on the proliferation of liver cancer cells was analyzed by MTT and clone formation assay. The regulatory relationship between KRT23 and AKT signaling pathway was analyzed by software prediction， qPCRand Western blot. Results The expression level of KRT23 in liver cancer was significantly higher than that in normal control tissues. MTT and clone formation assay showed that overexpression of KRT23 promoted cell proliferation， while downregulation of KRT23 inhibited cell proliferation. GSEA analysis， qPCR and Western blot showed that KRT23 could activate the AKT signaling pathway and promote cell proliferation. Meanwhile， inhibition of AKT signaling pathway in overexpressing KRT23 cells significantly reduced cell proliferation. Conclusions KRT23 affects the proliferation of liver cancer cells by regulating the AKT signaling pathway. These results suggest that KRT23 exerts significant effects upon the malignant procession of liver cancer and may play an important role in the occurrence and development of liver cancer. |
| first_indexed | 2024-04-24T12:13:28Z |
| format | Article |
| id | doaj.art-4ad72c4298c34940bd53bc789840f305 |
| institution | Directory Open Access Journal |
| issn | 0253-9802 |
| language | zho |
| last_indexed | 2024-04-24T12:13:28Z |
| publishDate | 2024-03-01 |
| publisher | Editorial Office of Journal of New Medicine |
| record_format | Article |
| series | Xin yixue |
| spelling | doaj.art-4ad72c4298c34940bd53bc789840f3052024-04-08T08:59:24ZzhoEditorial Office of Journal of New MedicineXin yixue0253-98022024-03-0155322222610.3969/j.issn.0253-9802.2024.03.013KRT23 promotes the proliferation of liver cancer by activating AKT signaling pathwayLiang Xinghua, Liu Foqiu, Yan Rong, Hu Juan0Department of Gastroenterology， the Fourth Affiliated Hospital of Guangzhou Medical University， Guangzhou 511300， ChinaObjective To investigate the effect of high keratin 23 （KRT23） expression promoting cell proliferation on liver cancer. Methods The expression of KRT23 in liver cancer was analyzed by bioinformatics. After overexpression and inhibition of KRT23， the effect of KRT23 on the proliferation of liver cancer cells was analyzed by MTT and clone formation assay. The regulatory relationship between KRT23 and AKT signaling pathway was analyzed by software prediction， qPCRand Western blot. Results The expression level of KRT23 in liver cancer was significantly higher than that in normal control tissues. MTT and clone formation assay showed that overexpression of KRT23 promoted cell proliferation， while downregulation of KRT23 inhibited cell proliferation. GSEA analysis， qPCR and Western blot showed that KRT23 could activate the AKT signaling pathway and promote cell proliferation. Meanwhile， inhibition of AKT signaling pathway in overexpressing KRT23 cells significantly reduced cell proliferation. Conclusions KRT23 affects the proliferation of liver cancer cells by regulating the AKT signaling pathway. These results suggest that KRT23 exerts significant effects upon the malignant procession of liver cancer and may play an important role in the occurrence and development of liver cancer.https://www.xinyixue.cn/fileup/0253-9802/PDF/1712537348253-1480554338.pdfkrt23|akt signaling pathway|liver cancer|proliferation |
| spellingShingle | Liang Xinghua, Liu Foqiu, Yan Rong, Hu Juan KRT23 promotes the proliferation of liver cancer by activating AKT signaling pathway Xin yixue krt23|akt signaling pathway|liver cancer|proliferation |
| title | KRT23 promotes the proliferation of liver cancer by activating AKT signaling pathway |
| title_full | KRT23 promotes the proliferation of liver cancer by activating AKT signaling pathway |
| title_fullStr | KRT23 promotes the proliferation of liver cancer by activating AKT signaling pathway |
| title_full_unstemmed | KRT23 promotes the proliferation of liver cancer by activating AKT signaling pathway |
| title_short | KRT23 promotes the proliferation of liver cancer by activating AKT signaling pathway |
| title_sort | krt23 promotes the proliferation of liver cancer by activating akt signaling pathway |
| topic | krt23|akt signaling pathway|liver cancer|proliferation |
| url | https://www.xinyixue.cn/fileup/0253-9802/PDF/1712537348253-1480554338.pdf |
| work_keys_str_mv | AT liangxinghualiufoqiuyanronghujuan krt23promotestheproliferationoflivercancerbyactivatingaktsignalingpathway |