In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis
IntroductionIntegrin αvβ6, which is upregulated in malignancies and remains absent or weak in normal tissue, is a promising target in molecular imaging therapeutics. In vivo imaging of integrin αvβ6 could therefore be valuable for early tumor detection and intraoperative guidance.MethodsIn this stud...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-03-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1070967/full |
| _version_ | 1811155421917872128 |
|---|---|
| author | Di Fan Chengkai Zhang Qi Luo Baowang Li Lin Ai Deling Li Wang Jia |
| author_facet | Di Fan Chengkai Zhang Qi Luo Baowang Li Lin Ai Deling Li Wang Jia |
| author_sort | Di Fan |
| collection | DOAJ |
| description | IntroductionIntegrin αvβ6, which is upregulated in malignancies and remains absent or weak in normal tissue, is a promising target in molecular imaging therapeutics. In vivo imaging of integrin αvβ6 could therefore be valuable for early tumor detection and intraoperative guidance.MethodsIn this study, integrin αvβ6-targeting probe G2-SFLAP3 was labeled with near-infrared (NIR) dye Cy5.5 or radioisotope 68Ga. The resulting probes were evaluated in integrin αvβ6-positive A549 and αvβ6-negative H1703 xenograft mice models.ResultsThe cellar uptake of G2-SFLAP3-Cy5.5 was consistent with the expression of integrin αvβ6. Both subcutaneous and brain metastatic A549 tumors could be clearly visualized by NIR fluorescent imaging of G2-SFLAP3-Cy5.5. A549 tumors demonstrated the highest G2-SFLAP3-Cy5.5 accumulation at 4h post-injection (p.i.) and remain detectable at 84h p.i. The fluorescent signal of G2-SFLAP3-Cy5.5 was significantly reduced in H1703 and A549-blocking groups. Consistently, small-animal PET imaging showed tumor-specific accumulation of 68Ga-DOTA-G2-SFLAP3.DiscussionG2-SFLAP3 represents a promising agent for noninvasive imaging of non-small cell lung cancer (NSCLC) and brain metastases. |
| first_indexed | 2024-04-10T04:32:44Z |
| format | Article |
| id | doaj.art-4ad7787a687b45ffa3009165d4ff1a32 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-10T04:32:44Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-4ad7787a687b45ffa3009165d4ff1a322023-03-10T05:39:53ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-03-011310.3389/fonc.2023.10709671070967In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasisDi Fan0Chengkai Zhang1Qi Luo2Baowang Li3Lin Ai4Deling Li5Wang Jia6Department of Nuclear Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaGuangzhou Laboratory, Guangzhou International Bio Island, Guangzhou, Guangdong, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaDepartment of Nuclear Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaIntroductionIntegrin αvβ6, which is upregulated in malignancies and remains absent or weak in normal tissue, is a promising target in molecular imaging therapeutics. In vivo imaging of integrin αvβ6 could therefore be valuable for early tumor detection and intraoperative guidance.MethodsIn this study, integrin αvβ6-targeting probe G2-SFLAP3 was labeled with near-infrared (NIR) dye Cy5.5 or radioisotope 68Ga. The resulting probes were evaluated in integrin αvβ6-positive A549 and αvβ6-negative H1703 xenograft mice models.ResultsThe cellar uptake of G2-SFLAP3-Cy5.5 was consistent with the expression of integrin αvβ6. Both subcutaneous and brain metastatic A549 tumors could be clearly visualized by NIR fluorescent imaging of G2-SFLAP3-Cy5.5. A549 tumors demonstrated the highest G2-SFLAP3-Cy5.5 accumulation at 4h post-injection (p.i.) and remain detectable at 84h p.i. The fluorescent signal of G2-SFLAP3-Cy5.5 was significantly reduced in H1703 and A549-blocking groups. Consistently, small-animal PET imaging showed tumor-specific accumulation of 68Ga-DOTA-G2-SFLAP3.DiscussionG2-SFLAP3 represents a promising agent for noninvasive imaging of non-small cell lung cancer (NSCLC) and brain metastases.https://www.frontiersin.org/articles/10.3389/fonc.2023.1070967/fullintegrin αvβ6NSCLCbrain metastasesPETnear-infrared fluorescence imaging |
| spellingShingle | Di Fan Chengkai Zhang Qi Luo Baowang Li Lin Ai Deling Li Wang Jia In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis Frontiers in Oncology integrin αvβ6 NSCLC brain metastases PET near-infrared fluorescence imaging |
| title | In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis |
| title_full | In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis |
| title_fullStr | In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis |
| title_full_unstemmed | In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis |
| title_short | In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis |
| title_sort | in vivo evaluation of integrin αvβ6 targeting peptide in nsclc and brain metastasis |
| topic | integrin αvβ6 NSCLC brain metastases PET near-infrared fluorescence imaging |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1070967/full |
| work_keys_str_mv | AT difan invivoevaluationofintegrinavb6targetingpeptideinnsclcandbrainmetastasis AT chengkaizhang invivoevaluationofintegrinavb6targetingpeptideinnsclcandbrainmetastasis AT qiluo invivoevaluationofintegrinavb6targetingpeptideinnsclcandbrainmetastasis AT baowangli invivoevaluationofintegrinavb6targetingpeptideinnsclcandbrainmetastasis AT linai invivoevaluationofintegrinavb6targetingpeptideinnsclcandbrainmetastasis AT delingli invivoevaluationofintegrinavb6targetingpeptideinnsclcandbrainmetastasis AT wangjia invivoevaluationofintegrinavb6targetingpeptideinnsclcandbrainmetastasis |