Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions
Quantitative single-cell cultivation has provided fundamental contributions to our understanding of heterogeneity among industrially used microorganisms. Filamentous growing Streptomyces species are emerging platform organisms for industrial production processes, but their exploitation is still limi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02680/full |
| _version_ | 1819077231567175680 |
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| author | Joachim Koepff Christian Carsten Sachs Wolfgang Wiechert Wolfgang Wiechert Dietrich Kohlheyer Dietrich Kohlheyer Katharina Nöh Marco Oldiges Marco Oldiges Alexander Grünberger Alexander Grünberger |
| author_facet | Joachim Koepff Christian Carsten Sachs Wolfgang Wiechert Wolfgang Wiechert Dietrich Kohlheyer Dietrich Kohlheyer Katharina Nöh Marco Oldiges Marco Oldiges Alexander Grünberger Alexander Grünberger |
| author_sort | Joachim Koepff |
| collection | DOAJ |
| description | Quantitative single-cell cultivation has provided fundamental contributions to our understanding of heterogeneity among industrially used microorganisms. Filamentous growing Streptomyces species are emerging platform organisms for industrial production processes, but their exploitation is still limited due to often reported high batch-to-batch variations and unexpected growth and production differences. Population heterogeneity is suspected to be one responsible factor, which is so far not systematically investigated at the single-cell level. Novel microfluidic single-cell cultivation devices offer promising solutions to investigate these phenomena. In this study, we investigated the germination and growth behavior of Streptomyces lividans TK24 under varying medium compositions on different complexity levels (i.e., mycelial growth, hyphal growth and tip elongation) on single-cell level. Our analysis reveals a remarkable stability within growth and germination of spores and early mycelium development when exposed to constant and defined environments. We show that spores undergo long metabolic adaptation processes of up to > 30 h to adjust to new medium conditions, rather than using a “persister” strategy as a possibility to cope with rapidly changing environments. Due to this uniform behavior, we conclude that S. lividans can be cultivated quite robustly under constant environmental conditions as provided by microfluidic cultivation approaches. Failure and non-reproducible cultivations are thus most likely to be found in less controllable larger-scale cultivation workflows and as a result of environmental gradients within large-scale cultivations. |
| first_indexed | 2024-12-21T18:53:55Z |
| format | Article |
| id | doaj.art-4ad79da310ef477f8e3cb89602615105 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T18:53:55Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4ad79da310ef477f8e3cb896026151052022-12-21T18:53:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-11-01910.3389/fmicb.2018.02680418679Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium CompositionsJoachim Koepff0Christian Carsten Sachs1Wolfgang Wiechert2Wolfgang Wiechert3Dietrich Kohlheyer4Dietrich Kohlheyer5Katharina Nöh6Marco Oldiges7Marco Oldiges8Alexander Grünberger9Alexander Grünberger10Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, GermanyInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, GermanyInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, GermanyComputational Systems Biotechnology, RWTH Aachen University – Aachener Verfahrenstechnik, Aachen, GermanyInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, GermanyMicroscale Bioengineering, RWTH Aachen University – Aachener Verfahrenstechnik, Aachen, GermanyInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, GermanyInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, GermanyInstitute of Biotechnology, RWTH Aachen University, Aachen, GermanyInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, GermanyMultiscale Bioengineering, Bielefeld University, Bielefeld, GermanyQuantitative single-cell cultivation has provided fundamental contributions to our understanding of heterogeneity among industrially used microorganisms. Filamentous growing Streptomyces species are emerging platform organisms for industrial production processes, but their exploitation is still limited due to often reported high batch-to-batch variations and unexpected growth and production differences. Population heterogeneity is suspected to be one responsible factor, which is so far not systematically investigated at the single-cell level. Novel microfluidic single-cell cultivation devices offer promising solutions to investigate these phenomena. In this study, we investigated the germination and growth behavior of Streptomyces lividans TK24 under varying medium compositions on different complexity levels (i.e., mycelial growth, hyphal growth and tip elongation) on single-cell level. Our analysis reveals a remarkable stability within growth and germination of spores and early mycelium development when exposed to constant and defined environments. We show that spores undergo long metabolic adaptation processes of up to > 30 h to adjust to new medium conditions, rather than using a “persister” strategy as a possibility to cope with rapidly changing environments. Due to this uniform behavior, we conclude that S. lividans can be cultivated quite robustly under constant environmental conditions as provided by microfluidic cultivation approaches. Failure and non-reproducible cultivations are thus most likely to be found in less controllable larger-scale cultivation workflows and as a result of environmental gradients within large-scale cultivations.https://www.frontiersin.org/article/10.3389/fmicb.2018.02680/fullStreptomyces lividansfilamentous growthmicrofluidicssingle-cell cultivationgerminationtip elongation rate |
| spellingShingle | Joachim Koepff Christian Carsten Sachs Wolfgang Wiechert Wolfgang Wiechert Dietrich Kohlheyer Dietrich Kohlheyer Katharina Nöh Marco Oldiges Marco Oldiges Alexander Grünberger Alexander Grünberger Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions Frontiers in Microbiology Streptomyces lividans filamentous growth microfluidics single-cell cultivation germination tip elongation rate |
| title | Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions |
| title_full | Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions |
| title_fullStr | Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions |
| title_full_unstemmed | Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions |
| title_short | Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions |
| title_sort | germination and growth analysis of streptomyces lividans at the single cell level under varying medium compositions |
| topic | Streptomyces lividans filamentous growth microfluidics single-cell cultivation germination tip elongation rate |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02680/full |
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