A New Gene <i>SCY3</i> Homologous to <i>Scygonadin</i> Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of <i>Scylla paramamosain</i>
In the study, a new gene homologous to the known antimicrobial peptide <i>Scygonadin</i> was identified in mud crab <i>Scylla paramamosain</i> and named <i>SCY3</i>. The full-length sequences of cDNA and genomic DNA were determined. Similar to <i>Scygonadin&...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5689 |
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| author | Su Long Fangyi Chen Jishan Li Ying Yang Ke-Jian Wang |
| author_facet | Su Long Fangyi Chen Jishan Li Ying Yang Ke-Jian Wang |
| author_sort | Su Long |
| collection | DOAJ |
| description | In the study, a new gene homologous to the known antimicrobial peptide <i>Scygonadin</i> was identified in mud crab <i>Scylla paramamosain</i> and named <i>SCY3</i>. The full-length sequences of cDNA and genomic DNA were determined. Similar to <i>Scygonadin</i>, <i>SCY3</i> was dominantly expressed in the ejaculatory ducts of male crab and the spermatheca of post-mating females at mating. The mRNA expression was significantly up-regulated after stimulation by <i>Vibrio alginolyticus</i>, but not by <i>Staphylococcus aureus</i>. The recombinant protein rSCY3 had a killing effect on <i>Micrococcus luteus</i> and could improve the survival rate of mud crabs infected with <i>V. alginolyticus</i>. Further analysis showed that rSCY3 interacted with rSCY1 or rSCY2 using Surface Plasmon Resonance (SPR, a technology for detecting interactions between biomolecules using biosensor chips) and Mammalian Two-Hybrid (M2H, a way of detecting interactions between proteins in vivo). Moreover, the rSCY3 could significantly improve the sperm acrosome reaction (AR) of <i>S. paramamosain</i> and the results demonstrated that the binding of rSCY3, rSCY4, and rSCY5 to progesterone was a potential factor affecting the sperm AR by SCYs on. This study lays the foundation for further investigation on the molecular mechanism of SCYs involved in both immunity and physiological effects of <i>S. paramamosain</i>. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:24:53Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4ad98da4fd0a444b94e4d37e701be0692023-11-17T11:37:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246568910.3390/ijms24065689A New Gene <i>SCY3</i> Homologous to <i>Scygonadin</i> Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of <i>Scylla paramamosain</i>Su Long0Fangyi Chen1Jishan Li2Ying Yang3Ke-Jian Wang4State Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaIn the study, a new gene homologous to the known antimicrobial peptide <i>Scygonadin</i> was identified in mud crab <i>Scylla paramamosain</i> and named <i>SCY3</i>. The full-length sequences of cDNA and genomic DNA were determined. Similar to <i>Scygonadin</i>, <i>SCY3</i> was dominantly expressed in the ejaculatory ducts of male crab and the spermatheca of post-mating females at mating. The mRNA expression was significantly up-regulated after stimulation by <i>Vibrio alginolyticus</i>, but not by <i>Staphylococcus aureus</i>. The recombinant protein rSCY3 had a killing effect on <i>Micrococcus luteus</i> and could improve the survival rate of mud crabs infected with <i>V. alginolyticus</i>. Further analysis showed that rSCY3 interacted with rSCY1 or rSCY2 using Surface Plasmon Resonance (SPR, a technology for detecting interactions between biomolecules using biosensor chips) and Mammalian Two-Hybrid (M2H, a way of detecting interactions between proteins in vivo). Moreover, the rSCY3 could significantly improve the sperm acrosome reaction (AR) of <i>S. paramamosain</i> and the results demonstrated that the binding of rSCY3, rSCY4, and rSCY5 to progesterone was a potential factor affecting the sperm AR by SCYs on. This study lays the foundation for further investigation on the molecular mechanism of SCYs involved in both immunity and physiological effects of <i>S. paramamosain</i>.https://www.mdpi.com/1422-0067/24/6/5689<i>Scylla paramamosain</i>antimicrobial peptide<i>Scygonadin</i><i>SCY3</i>acrosome reaction |
| spellingShingle | Su Long Fangyi Chen Jishan Li Ying Yang Ke-Jian Wang A New Gene <i>SCY3</i> Homologous to <i>Scygonadin</i> Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of <i>Scylla paramamosain</i> International Journal of Molecular Sciences <i>Scylla paramamosain</i> antimicrobial peptide <i>Scygonadin</i> <i>SCY3</i> acrosome reaction |
| title | A New Gene <i>SCY3</i> Homologous to <i>Scygonadin</i> Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of <i>Scylla paramamosain</i> |
| title_full | A New Gene <i>SCY3</i> Homologous to <i>Scygonadin</i> Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of <i>Scylla paramamosain</i> |
| title_fullStr | A New Gene <i>SCY3</i> Homologous to <i>Scygonadin</i> Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of <i>Scylla paramamosain</i> |
| title_full_unstemmed | A New Gene <i>SCY3</i> Homologous to <i>Scygonadin</i> Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of <i>Scylla paramamosain</i> |
| title_short | A New Gene <i>SCY3</i> Homologous to <i>Scygonadin</i> Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of <i>Scylla paramamosain</i> |
| title_sort | new gene i scy3 i homologous to i scygonadin i showing antibacterial activity and a potential role in the sperm acrosome reaction of i scylla paramamosain i |
| topic | <i>Scylla paramamosain</i> antimicrobial peptide <i>Scygonadin</i> <i>SCY3</i> acrosome reaction |
| url | https://www.mdpi.com/1422-0067/24/6/5689 |
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