Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results
For the first time ever developed has been the method of identification of genovariants in Yersinia pestis strains belonging to the main subspecies using PCR with hybridization-fluorescent registration of results. A complex of DNA-targets, the most of which are newly detected ones, have been utilize...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2014-12-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/185 |
| _version_ | 1797220862200381440 |
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| author | E. G. Oglodin G. N. Odinokov K. A. Nikiforov L. M. Kukleva G. A. Eroshenko |
| author_facet | E. G. Oglodin G. N. Odinokov K. A. Nikiforov L. M. Kukleva G. A. Eroshenko |
| author_sort | E. G. Oglodin |
| collection | DOAJ |
| description | For the first time ever developed has been the method of identification of genovariants in Yersinia pestis strains belonging to the main subspecies using PCR with hybridization-fluorescent registration of results. A complex of DNA-targets, the most of which are newly detected ones, have been utilized to differentiate between genovariants of antique (0.ANT, 1.ANT, 2.ANT, 4.ANT), medievalis (2.MED, 2.MED0), and orientalis (1.ORI) biovars. Application of these targets in PCR with hybridization-fluorescent registration of results provides for a rapid, effective and reliable classifying of the strains of the genovariants, circulating in various geographical regions. Efficacy of the method has been validated via analysis of 110 Y. pestis strains, isolated in the territory of the Russian Federation, neighboring countries and beyond, including 37 strains of antique, 53 strains of medievalis, and 20 strains of orientalis biovars. |
| first_indexed | 2024-03-08T13:43:20Z |
| format | Article |
| id | doaj.art-4adab0132bc9419fb484f77c1a9c1101 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:56:17Z |
| publishDate | 2014-12-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-4adab0132bc9419fb484f77c1a9c11012024-04-05T16:47:10ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2014-12-0104525510.21055/0370-1069-2014-4-52-55185Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of ResultsE. G. Oglodin0G. N. Odinokov1K. A. Nikiforov2L. M. Kukleva3G. A. Eroshenko4Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”For the first time ever developed has been the method of identification of genovariants in Yersinia pestis strains belonging to the main subspecies using PCR with hybridization-fluorescent registration of results. A complex of DNA-targets, the most of which are newly detected ones, have been utilized to differentiate between genovariants of antique (0.ANT, 1.ANT, 2.ANT, 4.ANT), medievalis (2.MED, 2.MED0), and orientalis (1.ORI) biovars. Application of these targets in PCR with hybridization-fluorescent registration of results provides for a rapid, effective and reliable classifying of the strains of the genovariants, circulating in various geographical regions. Efficacy of the method has been validated via analysis of 110 Y. pestis strains, isolated in the territory of the Russian Federation, neighboring countries and beyond, including 37 strains of antique, 53 strains of medievalis, and 20 strains of orientalis biovars.https://journal.microbe.ru/jour/article/view/185возбудитель чумыосновной подвидгеновариантыпцр с гибридизационно-флуоресцентным учетом результатовplague agentthe main subspeciesgenovariantspcr with hybridization-fluorescent registration of results |
| spellingShingle | E. G. Oglodin G. N. Odinokov K. A. Nikiforov L. M. Kukleva G. A. Eroshenko Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results Проблемы особо опасных инфекций возбудитель чумы основной подвид геноварианты пцр с гибридизационно-флуоресцентным учетом результатов plague agent the main subspecies genovariants pcr with hybridization-fluorescent registration of results |
| title | Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results |
| title_full | Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results |
| title_fullStr | Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results |
| title_full_unstemmed | Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results |
| title_short | Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results |
| title_sort | identification of genovariants of yersinia pestis strains belonging to main subspecies using pcr with hybridization fluorescent registration of results |
| topic | возбудитель чумы основной подвид геноварианты пцр с гибридизационно-флуоресцентным учетом результатов plague agent the main subspecies genovariants pcr with hybridization-fluorescent registration of results |
| url | https://journal.microbe.ru/jour/article/view/185 |
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