Exploration of Analgesia with Tramadol in the Coxsackievirus B3 Myocarditis Mouse Model
Infection of mice with Coxsackievirus B3 (CVB3) triggers inflammation of the heart and this mouse model is commonly used to investigate underlying mechanisms and therapeutic aspects for viral myocarditis. Virus-triggered cytotoxicity and the activity of infiltrating immune cells contribute to cardia...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/7/1222 |
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| author | Sandra Pinkert Meike Kespohl Nicolas Kelm Ziya Kaya Arnd Heuser Karin Klingel Antje Beling |
| author_facet | Sandra Pinkert Meike Kespohl Nicolas Kelm Ziya Kaya Arnd Heuser Karin Klingel Antje Beling |
| author_sort | Sandra Pinkert |
| collection | DOAJ |
| description | Infection of mice with Coxsackievirus B3 (CVB3) triggers inflammation of the heart and this mouse model is commonly used to investigate underlying mechanisms and therapeutic aspects for viral myocarditis. Virus-triggered cytotoxicity and the activity of infiltrating immune cells contribute to cardiac tissue injury. In addition to cardiac manifestation, CVB3 causes cell death and inflammation in the pancreas. The resulting pancreatitis represents a severe burden and under such experimental conditions, analgesics may be supportive to improve the animals’ well-being. Notably, several known mechanisms exist by which analgesics can interfere with the immune system and thereby compromise the feasibility of the model. We set up a study aiming to improve animal welfare while ensuring model integrity and investigated how tramadol, an opioid, affects virus-induced pathogenicity and immune response in the heart. Tramadol was administered seven days prior to a CVB3 infection in C57BL/6 mice and treatment was continued until the day of analysis. Tramadol had no effect on the virus titer or viral pathogenicity in the heart tissue and the inflammatory response, a hallmark of myocardial injury, was maintained. Our results show that tramadol exerts no disruptive effects on the CVB3 myocarditis mouse model and, therefore, the demonstrated protocol should be considered as a general analgesic strategy for CVB3 infection. |
| first_indexed | 2024-03-10T10:05:27Z |
| format | Article |
| id | doaj.art-4adc994e783c4863956e41ca6b0bc33d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T10:05:27Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4adc994e783c4863956e41ca6b0bc33d2023-11-22T01:33:54ZengMDPI AGViruses1999-49152021-06-01137122210.3390/v13071222Exploration of Analgesia with Tramadol in the Coxsackievirus B3 Myocarditis Mouse ModelSandra Pinkert0Meike Kespohl1Nicolas Kelm2Ziya Kaya3Arnd Heuser4Karin Klingel5Antje Beling6Institute of Biochemistry, Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, GermanyInstitute of Biochemistry, Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, GermanyInstitute of Biochemistry, Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, GermanyKardiologie, Angiologie und Pneumologie, Medizinische Klinik für Innere Medizin III, Universitätsklinikum Heidelberg, 69120 Heidelberg, GermanyAnimal Phenotyping Platform, Max-Delbrueck-Center for Molecular Medicine, 13125 Berlin, GermanyCardiopathology, Institute for Pathology and Neuropathology, University Hospital Tuebingen, 72076 Tuebingen, GermanyInstitute of Biochemistry, Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, GermanyInfection of mice with Coxsackievirus B3 (CVB3) triggers inflammation of the heart and this mouse model is commonly used to investigate underlying mechanisms and therapeutic aspects for viral myocarditis. Virus-triggered cytotoxicity and the activity of infiltrating immune cells contribute to cardiac tissue injury. In addition to cardiac manifestation, CVB3 causes cell death and inflammation in the pancreas. The resulting pancreatitis represents a severe burden and under such experimental conditions, analgesics may be supportive to improve the animals’ well-being. Notably, several known mechanisms exist by which analgesics can interfere with the immune system and thereby compromise the feasibility of the model. We set up a study aiming to improve animal welfare while ensuring model integrity and investigated how tramadol, an opioid, affects virus-induced pathogenicity and immune response in the heart. Tramadol was administered seven days prior to a CVB3 infection in C57BL/6 mice and treatment was continued until the day of analysis. Tramadol had no effect on the virus titer or viral pathogenicity in the heart tissue and the inflammatory response, a hallmark of myocardial injury, was maintained. Our results show that tramadol exerts no disruptive effects on the CVB3 myocarditis mouse model and, therefore, the demonstrated protocol should be considered as a general analgesic strategy for CVB3 infection.https://www.mdpi.com/1999-4915/13/7/1222infectionmyocarditispancreatitisrefinementanalgesia |
| spellingShingle | Sandra Pinkert Meike Kespohl Nicolas Kelm Ziya Kaya Arnd Heuser Karin Klingel Antje Beling Exploration of Analgesia with Tramadol in the Coxsackievirus B3 Myocarditis Mouse Model Viruses infection myocarditis pancreatitis refinement analgesia |
| title | Exploration of Analgesia with Tramadol in the Coxsackievirus B3 Myocarditis Mouse Model |
| title_full | Exploration of Analgesia with Tramadol in the Coxsackievirus B3 Myocarditis Mouse Model |
| title_fullStr | Exploration of Analgesia with Tramadol in the Coxsackievirus B3 Myocarditis Mouse Model |
| title_full_unstemmed | Exploration of Analgesia with Tramadol in the Coxsackievirus B3 Myocarditis Mouse Model |
| title_short | Exploration of Analgesia with Tramadol in the Coxsackievirus B3 Myocarditis Mouse Model |
| title_sort | exploration of analgesia with tramadol in the coxsackievirus b3 myocarditis mouse model |
| topic | infection myocarditis pancreatitis refinement analgesia |
| url | https://www.mdpi.com/1999-4915/13/7/1222 |
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