Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of <i>Spodoptera exigua</i>
<i>Spodoptera exigua</i> is a worldwide pest afflicting edible vegetables and has developed varying levels of resistance to insecticides. Methoxyfenozide (MET), an ecdysteroid agonist, is effective against lepidopteran pests such as <i>S. exigua</i>. However, the mechanism of...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/9080 |
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| author | Zhixian Zhang Yajie Ma Xiaoyan Ma Hongyan Hu Dan Wang Xianpeng Song Xiangliang Ren Yan Ma |
| author_facet | Zhixian Zhang Yajie Ma Xiaoyan Ma Hongyan Hu Dan Wang Xianpeng Song Xiangliang Ren Yan Ma |
| author_sort | Zhixian Zhang |
| collection | DOAJ |
| description | <i>Spodoptera exigua</i> is a worldwide pest afflicting edible vegetables and has developed varying levels of resistance to insecticides. Methoxyfenozide (MET), an ecdysteroid agonist, is effective against lepidopteran pests such as <i>S. exigua</i>. However, the mechanism of MET to <i>S. exigua</i> remains unclear. In this study, we analyzed the expression patterns of genes related to the ecdysone signaling pathway in transcriptome data treated with sublethal doses of MET and analyzed how expression levels of key genes affect the toxicity of MET on <i>S. exigua</i>. Our results demonstrated that 2639 genes were up-regulated and 2512 genes were down-regulated in <i>S. exigua</i> treated with LC<sub>30</sub> of MET. Of these, 15 genes were involved in the ecdysone signaling pathway. qPCR results demonstrated that <i>ecdysone receptor A</i> (<i>EcRA</i>) expression levels significantly increased in <i>S. exigua</i> when treated with different doses of MET, and that the RNAi-mediated silencing of <i>EcRA</i> significantly increased mortality to 55.43% at 72 h when L3 <i>S. exigua</i> larvae were exposed to MET at the LC<sub>30</sub> dose. Additionally, knocking down <i>EcRA</i> suppressed the most genes expressed in the ecdysone signaling pathway. The combination of MET and ds<i>EcRA</i> affected the expression of <i>E74</i> and enhanced the expression of <i>TREA</i>. These results demonstrate that the adverse effects of sublethal MET disturb the ecdysone signaling pathway in <i>S. exigua</i>, and <i>EcRA</i> is closely related to MET toxic effect. This study increases our collective understanding of the mechanisms of MET in insect pests. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:43:47Z |
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| spelling | doaj.art-4add997f4b234eda93d25aaacd6595f52023-11-22T08:05:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216908010.3390/ijms22169080Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of <i>Spodoptera exigua</i>Zhixian Zhang0Yajie Ma1Xiaoyan Ma2Hongyan Hu3Dan Wang4Xianpeng Song5Xiangliang Ren6Yan Ma7Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, China<i>Spodoptera exigua</i> is a worldwide pest afflicting edible vegetables and has developed varying levels of resistance to insecticides. Methoxyfenozide (MET), an ecdysteroid agonist, is effective against lepidopteran pests such as <i>S. exigua</i>. However, the mechanism of MET to <i>S. exigua</i> remains unclear. In this study, we analyzed the expression patterns of genes related to the ecdysone signaling pathway in transcriptome data treated with sublethal doses of MET and analyzed how expression levels of key genes affect the toxicity of MET on <i>S. exigua</i>. Our results demonstrated that 2639 genes were up-regulated and 2512 genes were down-regulated in <i>S. exigua</i> treated with LC<sub>30</sub> of MET. Of these, 15 genes were involved in the ecdysone signaling pathway. qPCR results demonstrated that <i>ecdysone receptor A</i> (<i>EcRA</i>) expression levels significantly increased in <i>S. exigua</i> when treated with different doses of MET, and that the RNAi-mediated silencing of <i>EcRA</i> significantly increased mortality to 55.43% at 72 h when L3 <i>S. exigua</i> larvae were exposed to MET at the LC<sub>30</sub> dose. Additionally, knocking down <i>EcRA</i> suppressed the most genes expressed in the ecdysone signaling pathway. The combination of MET and ds<i>EcRA</i> affected the expression of <i>E74</i> and enhanced the expression of <i>TREA</i>. These results demonstrate that the adverse effects of sublethal MET disturb the ecdysone signaling pathway in <i>S. exigua</i>, and <i>EcRA</i> is closely related to MET toxic effect. This study increases our collective understanding of the mechanisms of MET in insect pests.https://www.mdpi.com/1422-0067/22/16/9080<i>Spodoptera exigua</i>methoxyfenozidetoxicityecdysone receptor Amortality |
| spellingShingle | Zhixian Zhang Yajie Ma Xiaoyan Ma Hongyan Hu Dan Wang Xianpeng Song Xiangliang Ren Yan Ma Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of <i>Spodoptera exigua</i> International Journal of Molecular Sciences <i>Spodoptera exigua</i> methoxyfenozide toxicity ecdysone receptor A mortality |
| title | Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of <i>Spodoptera exigua</i> |
| title_full | Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of <i>Spodoptera exigua</i> |
| title_fullStr | Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of <i>Spodoptera exigua</i> |
| title_full_unstemmed | Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of <i>Spodoptera exigua</i> |
| title_short | Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of <i>Spodoptera exigua</i> |
| title_sort | combined transcriptomic analysis and rna interference reveal the effects of methoxyfenozide on ecdysone signaling pathway of i spodoptera exigua i |
| topic | <i>Spodoptera exigua</i> methoxyfenozide toxicity ecdysone receptor A mortality |
| url | https://www.mdpi.com/1422-0067/22/16/9080 |
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