Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit
Ascorbate (vitamin C) deficiency leads to low immunity, scurvy, and other human diseases and is therefore a global health problem. Given that plants are major ascorbate sources for humans, biofortification of this vitamin in our foodstuffs is of considerable importance. Ascorbate is synthetized by a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00308/full |
| _version_ | 1818559938446753792 |
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| author | Sonia eOsorio Sonia eOsorio Adriano eNunes-Nesi Adriano eNunes-Nesi Marina eStratmann Alisdair eFernie |
| author_facet | Sonia eOsorio Sonia eOsorio Adriano eNunes-Nesi Adriano eNunes-Nesi Marina eStratmann Alisdair eFernie |
| author_sort | Sonia eOsorio |
| collection | DOAJ |
| description | Ascorbate (vitamin C) deficiency leads to low immunity, scurvy, and other human diseases and is therefore a global health problem. Given that plants are major ascorbate sources for humans, biofortification of this vitamin in our foodstuffs is of considerable importance. Ascorbate is synthetized by a number of alternative pathways: (i) from the glycolytic intermediates D-glucose-6P (the key intermediates are GDP-D-mannose and L-galactose), (ii) from the breakdown of the cell wall polymer pectin which uses the methyl ester of D-galacturonic acid as precursor and (iii) from myo-inositol as precursor via myo-inositol oxygenase. We report here the engineering of fruit-specific overexpression of a bacterial pyrophosphatase, which hydrolyzes the inorganic pyrophosphate (PPi) to orthophosphate (Pi). This strategy resulted in increased vitamin C levels up to 2.5 fold in ripe fruit as well as increasing in the major sugars, sucrose and glucose, yet decreasing the level of starch. When considered together, these finding indicate an intimate linkage between ascorbate and sugar biosynthesis in plants. Moreover, the combined data reveal the importance of PPi metabolism in tomato fruit metabolism and development. |
| first_indexed | 2024-12-14T00:32:02Z |
| format | Article |
| id | doaj.art-4ae0f92285fe42cbb0f114fbfde203ee |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T00:32:02Z |
| publishDate | 2013-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-4ae0f92285fe42cbb0f114fbfde203ee2022-12-21T23:24:48ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2013-08-01410.3389/fpls.2013.0030851051Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruitSonia eOsorio0Sonia eOsorio1Adriano eNunes-Nesi2Adriano eNunes-Nesi3Marina eStratmann4Alisdair eFernie5Málaga UniversityMax-Planck-Institut für Molekulare PflanzenphysiologieMax-Planck-Institut für Molekulare PflanzenphysiologieUniversidade Federal de ViçosaMax-Planck-Institut für Molekulare PflanzenphysiologieMax-Planck-Institut für Molekulare PflanzenphysiologieAscorbate (vitamin C) deficiency leads to low immunity, scurvy, and other human diseases and is therefore a global health problem. Given that plants are major ascorbate sources for humans, biofortification of this vitamin in our foodstuffs is of considerable importance. Ascorbate is synthetized by a number of alternative pathways: (i) from the glycolytic intermediates D-glucose-6P (the key intermediates are GDP-D-mannose and L-galactose), (ii) from the breakdown of the cell wall polymer pectin which uses the methyl ester of D-galacturonic acid as precursor and (iii) from myo-inositol as precursor via myo-inositol oxygenase. We report here the engineering of fruit-specific overexpression of a bacterial pyrophosphatase, which hydrolyzes the inorganic pyrophosphate (PPi) to orthophosphate (Pi). This strategy resulted in increased vitamin C levels up to 2.5 fold in ripe fruit as well as increasing in the major sugars, sucrose and glucose, yet decreasing the level of starch. When considered together, these finding indicate an intimate linkage between ascorbate and sugar biosynthesis in plants. Moreover, the combined data reveal the importance of PPi metabolism in tomato fruit metabolism and development.http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00308/fullripeningSugarsascorbatetomato fruitpyrophosphatase |
| spellingShingle | Sonia eOsorio Sonia eOsorio Adriano eNunes-Nesi Adriano eNunes-Nesi Marina eStratmann Alisdair eFernie Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit Frontiers in Plant Science ripening Sugars ascorbate tomato fruit pyrophosphatase |
| title | Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_full | Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_fullStr | Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_full_unstemmed | Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_short | Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_sort | pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| topic | ripening Sugars ascorbate tomato fruit pyrophosphatase |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00308/full |
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