Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancer
Abstract Background The detection of circulating tumor DNA (ctDNA) in colorectal cancer (CRC) by liquid biopsy may have prognostic information. In this perioperative study, we evaluate if there is a relationship between mutant allele frequency (MAF) of Kirsten rat sarcoma viral oncogene homolog (KRA...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-08-01
|
| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.4677 |
| _version_ | 1818486402279538688 |
|---|---|
| author | Takaomi Hayashi Yoichiro Yoshida Teppei Yamada Keita Tanaka Hideki Shimaoka Ryuji Kajitani Taro Munechika Hideki Nagano Yoshiko Matsumoto Akira Komono Ryohei Sakamoto Naoya Aisu Gumpei Yoshimatsu Fumihiro Yoshimura Suguru Hasegawa |
| author_facet | Takaomi Hayashi Yoichiro Yoshida Teppei Yamada Keita Tanaka Hideki Shimaoka Ryuji Kajitani Taro Munechika Hideki Nagano Yoshiko Matsumoto Akira Komono Ryohei Sakamoto Naoya Aisu Gumpei Yoshimatsu Fumihiro Yoshimura Suguru Hasegawa |
| author_sort | Takaomi Hayashi |
| collection | DOAJ |
| description | Abstract Background The detection of circulating tumor DNA (ctDNA) in colorectal cancer (CRC) by liquid biopsy may have prognostic information. In this perioperative study, we evaluate if there is a relationship between mutant allele frequency (MAF) of Kirsten rat sarcoma viral oncogene homolog (KRAS) and tumor recurrence and how that could be useful in the early detection of recurrence. Methods Among 304 cases of colorectal cancer surgery, ctDNA was sampled from the perioperative blood of 84 patients with CRC with KRAS mutation (exon 4 p.A146T, exon 4 p.A146V, exon 2 p.G12A, exon 2 p.G12C, exon 2 p.G12D, exon 2 p.G12S, exon 2 p.G12V, exon 2 p.G13D, exon 3 p.Q61H) and analyzed using the digital polymerase chain reaction system. The median observation period was 26 months. Results Although the relationship between the perioperative MAF of KRAS and recurrence was not proved, tumor diameter, tumor depth, and stage were correlated with the preoperative MAF of KRAS (p = 0.034, p = 0.002, p = 0.008). However, tumor diameter, tumor depth, and stage did not correlate with MAF of KRAS at postoperative day 30. Conclusions In this study, pathological tumor size, tumor depth, and stage were correlated with preoperative MAF of KRAS, but it was unreliable to predict recurrence by detection of ctDNA with KRAS mutation in the perioperative period of colorectal surgery. |
| first_indexed | 2024-12-10T16:22:28Z |
| format | Article |
| id | doaj.art-4ae45131eee642858b6ffd925114faa2 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-12-10T16:22:28Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-4ae45131eee642858b6ffd925114faa22022-12-22T01:41:46ZengWileyCancer Medicine2045-76342022-08-0111163126313510.1002/cam4.4677Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancerTakaomi Hayashi0Yoichiro Yoshida1Teppei Yamada2Keita Tanaka3Hideki Shimaoka4Ryuji Kajitani5Taro Munechika6Hideki Nagano7Yoshiko Matsumoto8Akira Komono9Ryohei Sakamoto10Naoya Aisu11Gumpei Yoshimatsu12Fumihiro Yoshimura13Suguru Hasegawa14Department of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanDepartment of Gastroenterological Surgery Fukuoka University Faculty of Medicine Fukuoka JapanAbstract Background The detection of circulating tumor DNA (ctDNA) in colorectal cancer (CRC) by liquid biopsy may have prognostic information. In this perioperative study, we evaluate if there is a relationship between mutant allele frequency (MAF) of Kirsten rat sarcoma viral oncogene homolog (KRAS) and tumor recurrence and how that could be useful in the early detection of recurrence. Methods Among 304 cases of colorectal cancer surgery, ctDNA was sampled from the perioperative blood of 84 patients with CRC with KRAS mutation (exon 4 p.A146T, exon 4 p.A146V, exon 2 p.G12A, exon 2 p.G12C, exon 2 p.G12D, exon 2 p.G12S, exon 2 p.G12V, exon 2 p.G13D, exon 3 p.Q61H) and analyzed using the digital polymerase chain reaction system. The median observation period was 26 months. Results Although the relationship between the perioperative MAF of KRAS and recurrence was not proved, tumor diameter, tumor depth, and stage were correlated with the preoperative MAF of KRAS (p = 0.034, p = 0.002, p = 0.008). However, tumor diameter, tumor depth, and stage did not correlate with MAF of KRAS at postoperative day 30. Conclusions In this study, pathological tumor size, tumor depth, and stage were correlated with preoperative MAF of KRAS, but it was unreliable to predict recurrence by detection of ctDNA with KRAS mutation in the perioperative period of colorectal surgery.https://doi.org/10.1002/cam4.4677circulating tumor DNAcolorectal cancerdigital PCRKRASliquid biopsy |
| spellingShingle | Takaomi Hayashi Yoichiro Yoshida Teppei Yamada Keita Tanaka Hideki Shimaoka Ryuji Kajitani Taro Munechika Hideki Nagano Yoshiko Matsumoto Akira Komono Ryohei Sakamoto Naoya Aisu Gumpei Yoshimatsu Fumihiro Yoshimura Suguru Hasegawa Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancer Cancer Medicine circulating tumor DNA colorectal cancer digital PCR KRAS liquid biopsy |
| title | Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancer |
| title_full | Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancer |
| title_fullStr | Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancer |
| title_full_unstemmed | Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancer |
| title_short | Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancer |
| title_sort | relationship between perioperative oncological evaluation and recurrence using circulating tumor dna with kras mutation in patients with colorectal cancer |
| topic | circulating tumor DNA colorectal cancer digital PCR KRAS liquid biopsy |
| url | https://doi.org/10.1002/cam4.4677 |
| work_keys_str_mv | AT takaomihayashi relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT yoichiroyoshida relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT teppeiyamada relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT keitatanaka relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT hidekishimaoka relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT ryujikajitani relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT taromunechika relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT hidekinagano relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT yoshikomatsumoto relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT akirakomono relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT ryoheisakamoto relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT naoyaaisu relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT gumpeiyoshimatsu relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT fumihiroyoshimura relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer AT suguruhasegawa relationshipbetweenperioperativeoncologicalevaluationandrecurrenceusingcirculatingtumordnawithkrasmutationinpatientswithcolorectalcancer |