ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging
In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clon...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-09-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/13/9/11913 |
| _version_ | 1798002557496328192 |
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| author | Toemsak Srikhirin Armote Somboonkaew Ratthasart Amarit Boonsong Sutapun Mongkol Kunakorn Pimpun Kitpoka Krisda Sudprasert Patjaree Peungthum Nongluck Houngkamhang Apirom Vongsakulyanon |
| author_facet | Toemsak Srikhirin Armote Somboonkaew Ratthasart Amarit Boonsong Sutapun Mongkol Kunakorn Pimpun Kitpoka Krisda Sudprasert Patjaree Peungthum Nongluck Houngkamhang Apirom Vongsakulyanon |
| author_sort | Toemsak Srikhirin |
| collection | DOAJ |
| description | In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC) samples were applied to a multichannel flow cell that was orthogonal to the detection line arrays for blood group typing. We found that the blood samples were correctly grouped in less than 12 min by the SPR imaging technique, and the results were consistent with those of the standard agglutination technique for all 60 samples. We found that mixed clones of antibodies provided 33%–68% greater change in the SPR signal than the single-clone antibodies. Applying the SPR imaging technique using readily available antibodies may reduce the costs of the antibodies, shorten the measurement time, and increase the throughput. |
| first_indexed | 2024-04-11T11:54:00Z |
| format | Article |
| id | doaj.art-4ae4b8fe9e0f47e8bb97407da1a73c09 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T11:54:00Z |
| publishDate | 2013-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-4ae4b8fe9e0f47e8bb97407da1a73c092022-12-22T04:25:12ZengMDPI AGSensors1424-82202013-09-01139119131192210.3390/s130911913ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance ImagingToemsak SrikhirinArmote SomboonkaewRatthasart AmaritBoonsong SutapunMongkol KunakornPimpun KitpokaKrisda SudprasertPatjaree PeungthumNongluck HoungkamhangApirom VongsakulyanonIn this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC) samples were applied to a multichannel flow cell that was orthogonal to the detection line arrays for blood group typing. We found that the blood samples were correctly grouped in less than 12 min by the SPR imaging technique, and the results were consistent with those of the standard agglutination technique for all 60 samples. We found that mixed clones of antibodies provided 33%–68% greater change in the SPR signal than the single-clone antibodies. Applying the SPR imaging technique using readily available antibodies may reduce the costs of the antibodies, shorten the measurement time, and increase the throughput.http://www.mdpi.com/1424-8220/13/9/11913ABO blood typingsurface plasmon resonance imaging (SPR imaging)immunoassayantibody arrays |
| spellingShingle | Toemsak Srikhirin Armote Somboonkaew Ratthasart Amarit Boonsong Sutapun Mongkol Kunakorn Pimpun Kitpoka Krisda Sudprasert Patjaree Peungthum Nongluck Houngkamhang Apirom Vongsakulyanon ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging Sensors ABO blood typing surface plasmon resonance imaging (SPR imaging) immunoassay antibody arrays |
| title | ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging |
| title_full | ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging |
| title_fullStr | ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging |
| title_full_unstemmed | ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging |
| title_short | ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging |
| title_sort | abo blood typing using an antibody array technique based on surface plasmon resonance imaging |
| topic | ABO blood typing surface plasmon resonance imaging (SPR imaging) immunoassay antibody arrays |
| url | http://www.mdpi.com/1424-8220/13/9/11913 |
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