Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by <i>Varroa destructor</i> by Means of Computer Tomography
The honey bee (<i>Apis mellifera</i> L. 1778) is an essential element in maintaining the diversity of the biosphere and food production. One of its most important parasites is <i>Varroa destructor</i>, Anderson and Trueman, 2000, which plays a role in the vectoring of deforme...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/12/8/717 |
| _version_ | 1797523426165915648 |
|---|---|
| author | Tamás Sipos Tamás Donkó Ildikó Jócsák Sándor Keszthelyi |
| author_facet | Tamás Sipos Tamás Donkó Ildikó Jócsák Sándor Keszthelyi |
| author_sort | Tamás Sipos |
| collection | DOAJ |
| description | The honey bee (<i>Apis mellifera</i> L. 1778) is an essential element in maintaining the diversity of the biosphere and food production. One of its most important parasites is <i>Varroa destructor</i>, Anderson and Trueman, 2000, which plays a role in the vectoring of deformed wing virus (DWV) in honey bee colonies. Our aim was to measure the potential morphometric changes in the pre-imaginal stage of <i>A. mellifera</i> caused by varroosis by means of computed tomography, hence supplying evidence for the presumable role that <i>V. destructor</i> plays as a virus vector. Based on our results, the developmental disorders in honey bees that ensued during the pre-imaginal stages were evident. The total-body length and abdomen length of parasitized specimens were shorter than those of their intact companions. In addition, the calculated quotients of the total-body/abdomen, head/thorax, and head/abdomen in parasitized samples were significantly altered upon infestation. In our view, these phenotypical disorders can also be traced to viral infection mediated by parasitism, which was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) analysis. Capitalizing on a non-destructive method, our study reveals the deformation of the honey bee due to mite parasitism and the intermediary role this pest plays in viral infection, inside the brood cell. |
| first_indexed | 2024-03-10T08:42:47Z |
| format | Article |
| id | doaj.art-4ae9805886e14dcd9db410bb73853c5f |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T08:42:47Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-4ae9805886e14dcd9db410bb73853c5f2023-11-22T08:07:30ZengMDPI AGInsects2075-44502021-08-0112871710.3390/insects12080717Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by <i>Varroa destructor</i> by Means of Computer TomographyTamás Sipos0Tamás Donkó1Ildikó Jócsák2Sándor Keszthelyi3Department of Agronomy, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, S. Guba Str. 40, H-7400 Kaposvár, HungaryMedicopus Nonprofit Ltd., S. Guba Str. 40, H-7400 Kaposvár, HungaryDepartment of Agronomy, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, S. Guba Str. 40, H-7400 Kaposvár, HungaryDepartment of Agronomy, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, S. Guba Str. 40, H-7400 Kaposvár, HungaryThe honey bee (<i>Apis mellifera</i> L. 1778) is an essential element in maintaining the diversity of the biosphere and food production. One of its most important parasites is <i>Varroa destructor</i>, Anderson and Trueman, 2000, which plays a role in the vectoring of deformed wing virus (DWV) in honey bee colonies. Our aim was to measure the potential morphometric changes in the pre-imaginal stage of <i>A. mellifera</i> caused by varroosis by means of computed tomography, hence supplying evidence for the presumable role that <i>V. destructor</i> plays as a virus vector. Based on our results, the developmental disorders in honey bees that ensued during the pre-imaginal stages were evident. The total-body length and abdomen length of parasitized specimens were shorter than those of their intact companions. In addition, the calculated quotients of the total-body/abdomen, head/thorax, and head/abdomen in parasitized samples were significantly altered upon infestation. In our view, these phenotypical disorders can also be traced to viral infection mediated by parasitism, which was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) analysis. Capitalizing on a non-destructive method, our study reveals the deformation of the honey bee due to mite parasitism and the intermediary role this pest plays in viral infection, inside the brood cell.https://www.mdpi.com/2075-4450/12/8/717body part deformationcomputed tomographyhoney beenon-destructive analysisnon-invasive methodvarroosis |
| spellingShingle | Tamás Sipos Tamás Donkó Ildikó Jócsák Sándor Keszthelyi Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by <i>Varroa destructor</i> by Means of Computer Tomography Insects body part deformation computed tomography honey bee non-destructive analysis non-invasive method varroosis |
| title | Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by <i>Varroa destructor</i> by Means of Computer Tomography |
| title_full | Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by <i>Varroa destructor</i> by Means of Computer Tomography |
| title_fullStr | Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by <i>Varroa destructor</i> by Means of Computer Tomography |
| title_full_unstemmed | Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by <i>Varroa destructor</i> by Means of Computer Tomography |
| title_short | Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by <i>Varroa destructor</i> by Means of Computer Tomography |
| title_sort | study of morphological features in pre imaginal honey bee impaired by i varroa destructor i by means of computer tomography |
| topic | body part deformation computed tomography honey bee non-destructive analysis non-invasive method varroosis |
| url | https://www.mdpi.com/2075-4450/12/8/717 |
| work_keys_str_mv | AT tamassipos studyofmorphologicalfeaturesinpreimaginalhoneybeeimpairedbyivarroadestructoribymeansofcomputertomography AT tamasdonko studyofmorphologicalfeaturesinpreimaginalhoneybeeimpairedbyivarroadestructoribymeansofcomputertomography AT ildikojocsak studyofmorphologicalfeaturesinpreimaginalhoneybeeimpairedbyivarroadestructoribymeansofcomputertomography AT sandorkeszthelyi studyofmorphologicalfeaturesinpreimaginalhoneybeeimpairedbyivarroadestructoribymeansofcomputertomography |