Application of Bio-Friendly Formulations of Chitinase-Producing <i>Streptomyces cellulosae</i> Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by <i>Sclerotium rolfsii</i>
Of ten actinobacterial isolates, <i>Streptomyces cellulosae</i> Actino 48 exhibited the strongest suppression of <i>Sclerotium rolfsii</i> mycelium growth and the highest chitinase enzyme production (49.2 U L<sup>−1</sup> min<sup>−1</sup>). The interac...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/7/3/167 |
| _version_ | 1797395224431951872 |
|---|---|
| author | Gaber Abo-Zaid Ahmed Abdelkhalek Saleh Matar Mai Darwish Muhammad Abdel-Gayed |
| author_facet | Gaber Abo-Zaid Ahmed Abdelkhalek Saleh Matar Mai Darwish Muhammad Abdel-Gayed |
| author_sort | Gaber Abo-Zaid |
| collection | DOAJ |
| description | Of ten actinobacterial isolates, <i>Streptomyces cellulosae</i> Actino 48 exhibited the strongest suppression of <i>Sclerotium rolfsii</i> mycelium growth and the highest chitinase enzyme production (49.2 U L<sup>−1</sup> min<sup>−1</sup>). The interaction between Actino 48 and <i>S</i>. <i>rolfsii</i> was studied by scanning electron microscope (SEM), which revealed many abnormalities, malformations, and injuries of the hypha, with large loss of <i>S</i>. <i>rolfsii</i> mycelia density and mass. Three talc-based formulations with culture broth, cell-free supernatant, and cell pellet suspension of chitinase-producing Actino 48 were characterized using SEM, Fourier transform infrared spectroscopy (FTIR), and a particle size analyzer. All formulations were evaluated as biocontrol agents for reducing damping-off, root rot, and pods rot diseases of peanut caused by <i>S</i>. <i>rolfsii</i> under greenhouse and open-field conditions. The talc-based culture broth formulation was the most effective soil treatment, which decreased the percentage of peanut diseases under greenhouse and open-field conditions during two successive seasons. The culture broth formulation showed the highest increase in the dry weight of peanut shoots, root systems, and yielded pods. The transcriptional levels of three defense-related genes (<i>PR-1</i>, <i>PR-3</i>, and <i>POD</i>) were elevated in the culture broth formulation treatment compared with other formulations. Subsequently, the bio-friendly talc-based culture broth formulation of chitinase-producing Actino 48 could potentially be used as a biocontrol agent for controlling peanut soil-borne diseases caused by <i>S</i>. <i>rolfsii</i>. |
| first_indexed | 2024-03-09T00:31:22Z |
| format | Article |
| id | doaj.art-4af377d7412947469f3c8a3a1ba58885 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T00:31:22Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-4af377d7412947469f3c8a3a1ba588852023-12-11T18:28:59ZengMDPI AGJournal of Fungi2309-608X2021-02-017316710.3390/jof7030167Application of Bio-Friendly Formulations of Chitinase-Producing <i>Streptomyces cellulosae</i> Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by <i>Sclerotium rolfsii</i>Gaber Abo-Zaid0Ahmed Abdelkhalek1Saleh Matar2Mai Darwish3Muhammad Abdel-Gayed4Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, EgyptPlant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, EgyptBioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, EgyptBotany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria 21526, EgyptOnion, Garlic and Oil Crops Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, EgyptOf ten actinobacterial isolates, <i>Streptomyces cellulosae</i> Actino 48 exhibited the strongest suppression of <i>Sclerotium rolfsii</i> mycelium growth and the highest chitinase enzyme production (49.2 U L<sup>−1</sup> min<sup>−1</sup>). The interaction between Actino 48 and <i>S</i>. <i>rolfsii</i> was studied by scanning electron microscope (SEM), which revealed many abnormalities, malformations, and injuries of the hypha, with large loss of <i>S</i>. <i>rolfsii</i> mycelia density and mass. Three talc-based formulations with culture broth, cell-free supernatant, and cell pellet suspension of chitinase-producing Actino 48 were characterized using SEM, Fourier transform infrared spectroscopy (FTIR), and a particle size analyzer. All formulations were evaluated as biocontrol agents for reducing damping-off, root rot, and pods rot diseases of peanut caused by <i>S</i>. <i>rolfsii</i> under greenhouse and open-field conditions. The talc-based culture broth formulation was the most effective soil treatment, which decreased the percentage of peanut diseases under greenhouse and open-field conditions during two successive seasons. The culture broth formulation showed the highest increase in the dry weight of peanut shoots, root systems, and yielded pods. The transcriptional levels of three defense-related genes (<i>PR-1</i>, <i>PR-3</i>, and <i>POD</i>) were elevated in the culture broth formulation treatment compared with other formulations. Subsequently, the bio-friendly talc-based culture broth formulation of chitinase-producing Actino 48 could potentially be used as a biocontrol agent for controlling peanut soil-borne diseases caused by <i>S</i>. <i>rolfsii</i>.https://www.mdpi.com/2309-608X/7/3/167peanut<i>Sclerotium rolfsii</i><i>Streptomyces cellulosae</i>chitinasebio-friendly formulationsbiocontrol |
| spellingShingle | Gaber Abo-Zaid Ahmed Abdelkhalek Saleh Matar Mai Darwish Muhammad Abdel-Gayed Application of Bio-Friendly Formulations of Chitinase-Producing <i>Streptomyces cellulosae</i> Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by <i>Sclerotium rolfsii</i> Journal of Fungi peanut <i>Sclerotium rolfsii</i> <i>Streptomyces cellulosae</i> chitinase bio-friendly formulations biocontrol |
| title | Application of Bio-Friendly Formulations of Chitinase-Producing <i>Streptomyces cellulosae</i> Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by <i>Sclerotium rolfsii</i> |
| title_full | Application of Bio-Friendly Formulations of Chitinase-Producing <i>Streptomyces cellulosae</i> Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by <i>Sclerotium rolfsii</i> |
| title_fullStr | Application of Bio-Friendly Formulations of Chitinase-Producing <i>Streptomyces cellulosae</i> Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by <i>Sclerotium rolfsii</i> |
| title_full_unstemmed | Application of Bio-Friendly Formulations of Chitinase-Producing <i>Streptomyces cellulosae</i> Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by <i>Sclerotium rolfsii</i> |
| title_short | Application of Bio-Friendly Formulations of Chitinase-Producing <i>Streptomyces cellulosae</i> Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by <i>Sclerotium rolfsii</i> |
| title_sort | application of bio friendly formulations of chitinase producing i streptomyces cellulosae i actino 48 for controlling peanut soil borne diseases caused by i sclerotium rolfsii i |
| topic | peanut <i>Sclerotium rolfsii</i> <i>Streptomyces cellulosae</i> chitinase bio-friendly formulations biocontrol |
| url | https://www.mdpi.com/2309-608X/7/3/167 |
| work_keys_str_mv | AT gaberabozaid applicationofbiofriendlyformulationsofchitinaseproducingistreptomycescellulosaeiactino48forcontrollingpeanutsoilbornediseasescausedbyisclerotiumrolfsiii AT ahmedabdelkhalek applicationofbiofriendlyformulationsofchitinaseproducingistreptomycescellulosaeiactino48forcontrollingpeanutsoilbornediseasescausedbyisclerotiumrolfsiii AT salehmatar applicationofbiofriendlyformulationsofchitinaseproducingistreptomycescellulosaeiactino48forcontrollingpeanutsoilbornediseasescausedbyisclerotiumrolfsiii AT maidarwish applicationofbiofriendlyformulationsofchitinaseproducingistreptomycescellulosaeiactino48forcontrollingpeanutsoilbornediseasescausedbyisclerotiumrolfsiii AT muhammadabdelgayed applicationofbiofriendlyformulationsofchitinaseproducingistreptomycescellulosaeiactino48forcontrollingpeanutsoilbornediseasescausedbyisclerotiumrolfsiii |