Recent advances in microbiological and molecular biological detection techniques of tuberculous meningitis
Tuberculous meningitis (TBM) is the most common type of central nervous system tuberculosis (TB) and has the highest mortality and disability rate. Early diagnosis is key to improving the prognosis and survival rate of patients. However, laboratory diagnosis of TBM is often difficult due to its pauc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1202752/full |
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| author | Wen-Feng Cao Wen-Feng Cao Er-Ling Leng Shi-Min Liu Shi-Min Liu Yong-Liang Zhou Yong-Liang Zhou Chao-Qun Luo Chao-Qun Luo Zheng-Bing Xiang Zheng-Bing Xiang Wen Cai Wen Cai Wei Rao Wei Rao Fan Hu Fan Hu Ping Zhang Ping Zhang An Wen An Wen |
| author_facet | Wen-Feng Cao Wen-Feng Cao Er-Ling Leng Shi-Min Liu Shi-Min Liu Yong-Liang Zhou Yong-Liang Zhou Chao-Qun Luo Chao-Qun Luo Zheng-Bing Xiang Zheng-Bing Xiang Wen Cai Wen Cai Wei Rao Wei Rao Fan Hu Fan Hu Ping Zhang Ping Zhang An Wen An Wen |
| author_sort | Wen-Feng Cao |
| collection | DOAJ |
| description | Tuberculous meningitis (TBM) is the most common type of central nervous system tuberculosis (TB) and has the highest mortality and disability rate. Early diagnosis is key to improving the prognosis and survival rate of patients. However, laboratory diagnosis of TBM is often difficult due to its paucibacillary nature and sub optimal sensitivity of conventional microbiology and molecular tools which often fails to detect the pathogen. The gold standard for TBM diagnosis is the presence of MTB in the CSF. The recognised methods for the identification of MTB are acid-fast bacilli (AFB) detected under CSF smear microscopy, MTB cultured in CSF, and MTB detected by polymerase chain reaction (PCR). Currently, many studies consider that all diagnostic techniques for TBM are not perfect, and no single technique is considered simple, fast, cheap, and efficient. A definite diagnosis of TBM is still difficult in current clinical practice. In this review, we summarise the current state of microbiological and molecular biological diagnostics for TBM, the latest advances in research, and discuss the advantages of these techniques, as well as the issues and challenges faced in terms of diagnostic effectiveness, laboratory infrastructure, testing costs, and clinical expertise, for clinicians to select appropriate testing methods. |
| first_indexed | 2024-03-12T13:06:15Z |
| format | Article |
| id | doaj.art-4af6ccd4114d473b99494d1a90710e26 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T13:06:15Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4af6ccd4114d473b99494d1a90710e262023-08-28T15:09:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12027521202752Recent advances in microbiological and molecular biological detection techniques of tuberculous meningitisWen-Feng Cao0Wen-Feng Cao1Er-Ling Leng2Shi-Min Liu3Shi-Min Liu4Yong-Liang Zhou5Yong-Liang Zhou6Chao-Qun Luo7Chao-Qun Luo8Zheng-Bing Xiang9Zheng-Bing Xiang10Wen Cai11Wen Cai12Wei Rao13Wei Rao14Fan Hu15Fan Hu16Ping Zhang17Ping Zhang18An Wen19An Wen20Department of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Pediatrics, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaDepartment of Neurology, Jiangxi Provincial People’s Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, Jiangxi, ChinaDepartment of neurology, Xiangya Hospital, Central South University, Jiangxi Hospital, National Regional Center for Neurological Diseases, Nanchang, Jiangxi, ChinaTuberculous meningitis (TBM) is the most common type of central nervous system tuberculosis (TB) and has the highest mortality and disability rate. Early diagnosis is key to improving the prognosis and survival rate of patients. However, laboratory diagnosis of TBM is often difficult due to its paucibacillary nature and sub optimal sensitivity of conventional microbiology and molecular tools which often fails to detect the pathogen. The gold standard for TBM diagnosis is the presence of MTB in the CSF. The recognised methods for the identification of MTB are acid-fast bacilli (AFB) detected under CSF smear microscopy, MTB cultured in CSF, and MTB detected by polymerase chain reaction (PCR). Currently, many studies consider that all diagnostic techniques for TBM are not perfect, and no single technique is considered simple, fast, cheap, and efficient. A definite diagnosis of TBM is still difficult in current clinical practice. In this review, we summarise the current state of microbiological and molecular biological diagnostics for TBM, the latest advances in research, and discuss the advantages of these techniques, as well as the issues and challenges faced in terms of diagnostic effectiveness, laboratory infrastructure, testing costs, and clinical expertise, for clinicians to select appropriate testing methods.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1202752/fulltuberculous meningitisMycobacterium tuberculosismicrobiologymolecular biologyresearch progress |
| spellingShingle | Wen-Feng Cao Wen-Feng Cao Er-Ling Leng Shi-Min Liu Shi-Min Liu Yong-Liang Zhou Yong-Liang Zhou Chao-Qun Luo Chao-Qun Luo Zheng-Bing Xiang Zheng-Bing Xiang Wen Cai Wen Cai Wei Rao Wei Rao Fan Hu Fan Hu Ping Zhang Ping Zhang An Wen An Wen Recent advances in microbiological and molecular biological detection techniques of tuberculous meningitis Frontiers in Microbiology tuberculous meningitis Mycobacterium tuberculosis microbiology molecular biology research progress |
| title | Recent advances in microbiological and molecular biological detection techniques of tuberculous meningitis |
| title_full | Recent advances in microbiological and molecular biological detection techniques of tuberculous meningitis |
| title_fullStr | Recent advances in microbiological and molecular biological detection techniques of tuberculous meningitis |
| title_full_unstemmed | Recent advances in microbiological and molecular biological detection techniques of tuberculous meningitis |
| title_short | Recent advances in microbiological and molecular biological detection techniques of tuberculous meningitis |
| title_sort | recent advances in microbiological and molecular biological detection techniques of tuberculous meningitis |
| topic | tuberculous meningitis Mycobacterium tuberculosis microbiology molecular biology research progress |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1202752/full |
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