Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[S]
An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly i...
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| Format: | Article |
| Language: | English |
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Elsevier
2010-09-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520423144 |
| _version_ | 1818740575000592384 |
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| author | Honggang Nie Ranran Liu Youyou Yang Yu Bai Yafeng Guan Daqing Qian Tao Wang Huwei Liu |
| author_facet | Honggang Nie Ranran Liu Youyou Yang Yu Bai Yafeng Guan Daqing Qian Tao Wang Huwei Liu |
| author_sort | Honggang Nie |
| collection | DOAJ |
| description | An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivity. Different lipid classes were separated on a normal-phase column in the first dimension and lipid molecular species were separated on a reversed-phase column in the second dimension, so that the ion suppression effects were reduced while the detection sensitivity was improved. Identified were 721 endogenous lipid species from 12 lipid classes, in which 415 structures were confirmed using tandem mass spectra, and the other 306 lipid molecular species were identified by accurate masses. The linearity, limit of detection, and repeatability were all satisfactory. The method was applied to the investigation of the lipid changes in rat peritoneal surface layer after peritoneal dialysis, and 32 potential lipid biomarkers were identified, as their concentrations in the dosed group were 2.2–12.5 times of those in the control group. The results revealed that this 2D LC-MS system was a promising tool for lipid profiling of complex biological samples. |
| first_indexed | 2024-12-18T01:42:54Z |
| format | Article |
| id | doaj.art-4af842d4c6aa47a0b41bfc040ec302a2 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T01:42:54Z |
| publishDate | 2010-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-4af842d4c6aa47a0b41bfc040ec302a22022-12-21T21:25:16ZengElsevierJournal of Lipid Research0022-22752010-09-0151928332844Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[S]Honggang Nie0Ranran Liu1Youyou Yang2Yu Bai3Yafeng Guan4Daqing Qian5Tao Wang6Huwei Liu7Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China; Ministry of Education (MOE) Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Peking University, Beijing, China; Institute of Analytical Chemistry, Peking University, Beijing, ChinaBeijing National Laboratory for Molecular Sciences, Peking University, Beijing, China; Ministry of Education (MOE) Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Peking University, Beijing, China; Institute of Analytical Chemistry, Peking University, Beijing, ChinaBeijing National Laboratory for Molecular Sciences, Peking University, Beijing, China; Ministry of Education (MOE) Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Peking University, Beijing, China; Institute of Analytical Chemistry, Peking University, Beijing, ChinaBeijing National Laboratory for Molecular Sciences, Peking University, Beijing, China; Ministry of Education (MOE) Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Peking University, Beijing, China; Institute of Analytical Chemistry, Peking University, Beijing, ChinaDepartment of Instrumental and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, ChinaDepartment of Medicine, Wannan Medical College, Wuhu, Anhui, ChinaDivision of Nephrology, Peking University Third Hospital, Beijing, ChinaTo whom correspondence should be addressed. hwliu@pku.edu.cn; Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China; Ministry of Education (MOE) Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Peking University, Beijing, China; Institute of Analytical Chemistry, Peking University, Beijing, ChinaAn online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivity. Different lipid classes were separated on a normal-phase column in the first dimension and lipid molecular species were separated on a reversed-phase column in the second dimension, so that the ion suppression effects were reduced while the detection sensitivity was improved. Identified were 721 endogenous lipid species from 12 lipid classes, in which 415 structures were confirmed using tandem mass spectra, and the other 306 lipid molecular species were identified by accurate masses. The linearity, limit of detection, and repeatability were all satisfactory. The method was applied to the investigation of the lipid changes in rat peritoneal surface layer after peritoneal dialysis, and 32 potential lipid biomarkers were identified, as their concentrations in the dosed group were 2.2–12.5 times of those in the control group. The results revealed that this 2D LC-MS system was a promising tool for lipid profiling of complex biological samples.http://www.sciencedirect.com/science/article/pii/S0022227520423144lipidomicfree fatty acidphospholipidlyso-phospholipidplasmalogenperitoneal dialysis |
| spellingShingle | Honggang Nie Ranran Liu Youyou Yang Yu Bai Yafeng Guan Daqing Qian Tao Wang Huwei Liu Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[S] Journal of Lipid Research lipidomic free fatty acid phospholipid lyso-phospholipid plasmalogen peritoneal dialysis |
| title | Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[S] |
| title_full | Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[S] |
| title_fullStr | Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[S] |
| title_full_unstemmed | Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[S] |
| title_short | Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[S] |
| title_sort | lipid profiling of rat peritoneal surface layers by online normal and reversed phase 2d lc qtof ms s |
| topic | lipidomic free fatty acid phospholipid lyso-phospholipid plasmalogen peritoneal dialysis |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520423144 |
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