The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitate-induced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells
Aim:We aimed to investigate the effect of rosmarinic acid (RA) on cell viability, steatosis, paraoxonase (PON)1, and PON3 protein levels in palmitate-induced non-alcoholic fatty liver disease (NAFLD) model in HepG2 cells.Materials and Methods:To induce an experimental steatosis model, HepG2 cells we...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Galenos Yayincilik
2021-08-01
|
| Series: | Namık Kemal Tıp Dergisi |
| Subjects: | |
| Online Access: |
http://namikkemalmedj.com/archives/archive-detail/article-preview/the-effect-of-rosmarinic-acid-on-cell-viability-st/48577
|
| _version_ | 1797916905323888640 |
|---|---|
| author | Esra YAKŞİ Eray ÖZGÜN |
| author_facet | Esra YAKŞİ Eray ÖZGÜN |
| author_sort | Esra YAKŞİ |
| collection | DOAJ |
| description | Aim:We aimed to investigate the effect of rosmarinic acid (RA) on cell viability, steatosis, paraoxonase (PON)1, and PON3 protein levels in palmitate-induced non-alcoholic fatty liver disease (NAFLD) model in HepG2 cells.Materials and Methods:To induce an experimental steatosis model, HepG2 cells were incubated with 1 mM palmitate for 24 hours. For the treatment, non-toxic RA concentrations were added to the cell culture medium simultaneously with the palmitate. Cell viability was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. To evaluate steatosis, intracellular triglyceride levels were measured and the cells were examined microscopically with Oil-Red O staining. PON1 and PON3 protein levels were measured by Western blotting.Results:1 mM palmitate caused a significant decrease in cell viability and a significant increase in triglyceride levels, but it did not significantly change PON1 and PON3 protein levels. RA caused a significant increase in cell viability and a significant decrease in triglyceride levels in the palmitate-treated cells. Similar findings with the triglyceride levels of cells were shown in microscopic examination of cells that were stained with Oil-Red O. RA did not significantly change PON1 and PON3 protein levels in neither non-treated cells nor treated cells with palmitate.Conclusion:Our study showed that RA increases cell viability and decreases steatosis, but it does not change PON1 and PON3 protein levels in palmitate-induced NAFLD model in HepG2 cells. |
| first_indexed | 2024-04-10T13:04:44Z |
| format | Article |
| id | doaj.art-4af874c138424075bfc43798f73ce481 |
| institution | Directory Open Access Journal |
| issn | 2587-0262 |
| language | English |
| last_indexed | 2024-04-10T13:04:44Z |
| publishDate | 2021-08-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Namık Kemal Tıp Dergisi |
| spelling | doaj.art-4af874c138424075bfc43798f73ce4812023-02-15T16:12:59ZengGalenos YayincilikNamık Kemal Tıp Dergisi2587-02622021-08-019213514210.4274/nkmj.galenos.2021.82051213049054The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitate-induced Non-alcoholic Fatty Liver Disease Model in HepG2 CellsEsra YAKŞİ0Eray ÖZGÜN1 Lüleburgaz Devlet Hastanesi, Kırklareli, Türkiye Trakya Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Edirne, Türkiye Aim:We aimed to investigate the effect of rosmarinic acid (RA) on cell viability, steatosis, paraoxonase (PON)1, and PON3 protein levels in palmitate-induced non-alcoholic fatty liver disease (NAFLD) model in HepG2 cells.Materials and Methods:To induce an experimental steatosis model, HepG2 cells were incubated with 1 mM palmitate for 24 hours. For the treatment, non-toxic RA concentrations were added to the cell culture medium simultaneously with the palmitate. Cell viability was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. To evaluate steatosis, intracellular triglyceride levels were measured and the cells were examined microscopically with Oil-Red O staining. PON1 and PON3 protein levels were measured by Western blotting.Results:1 mM palmitate caused a significant decrease in cell viability and a significant increase in triglyceride levels, but it did not significantly change PON1 and PON3 protein levels. RA caused a significant increase in cell viability and a significant decrease in triglyceride levels in the palmitate-treated cells. Similar findings with the triglyceride levels of cells were shown in microscopic examination of cells that were stained with Oil-Red O. RA did not significantly change PON1 and PON3 protein levels in neither non-treated cells nor treated cells with palmitate.Conclusion:Our study showed that RA increases cell viability and decreases steatosis, but it does not change PON1 and PON3 protein levels in palmitate-induced NAFLD model in HepG2 cells. http://namikkemalmedj.com/archives/archive-detail/article-preview/the-effect-of-rosmarinic-acid-on-cell-viability-st/48577 non-alcoholic fatty liver diseaserosmarinic acidpalmitateparaoxonase-1paraoxonase-3 |
| spellingShingle | Esra YAKŞİ Eray ÖZGÜN The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitate-induced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells Namık Kemal Tıp Dergisi non-alcoholic fatty liver disease rosmarinic acid palmitate paraoxonase-1 paraoxonase-3 |
| title | The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitate-induced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells |
| title_full | The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitate-induced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells |
| title_fullStr | The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitate-induced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells |
| title_full_unstemmed | The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitate-induced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells |
| title_short | The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitate-induced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells |
| title_sort | effect of rosmarinic acid on cell viability steatosis paraoxonase 1 and paraoxonase 3 protein levels in palmitate induced non alcoholic fatty liver disease model in hepg2 cells |
| topic | non-alcoholic fatty liver disease rosmarinic acid palmitate paraoxonase-1 paraoxonase-3 |
| url |
http://namikkemalmedj.com/archives/archive-detail/article-preview/the-effect-of-rosmarinic-acid-on-cell-viability-st/48577
|
| work_keys_str_mv | AT esrayaksi theeffectofrosmarinicacidoncellviabilitysteatosisparaoxonase1andparaoxonase3proteinlevelsinpalmitateinducednonalcoholicfattyliverdiseasemodelinhepg2cells AT erayozgun theeffectofrosmarinicacidoncellviabilitysteatosisparaoxonase1andparaoxonase3proteinlevelsinpalmitateinducednonalcoholicfattyliverdiseasemodelinhepg2cells AT esrayaksi effectofrosmarinicacidoncellviabilitysteatosisparaoxonase1andparaoxonase3proteinlevelsinpalmitateinducednonalcoholicfattyliverdiseasemodelinhepg2cells AT erayozgun effectofrosmarinicacidoncellviabilitysteatosisparaoxonase1andparaoxonase3proteinlevelsinpalmitateinducednonalcoholicfattyliverdiseasemodelinhepg2cells |