Simple prediction of COVID-19 convalescent plasma units with high levels of neutralization antibodies
Abstract Background Hyperimmune convalescent COVID-19 plasma (CCP) containing anti-SARS-CoV-2 neutralizing antibodies (NAbs) was proposed as a therapeutic option for patients early in the new coronavirus disease pandemic. The efficacy of this therapy depends on the quantity of neutralizing antibodie...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-023-02007-0 |
| _version_ | 1827974758437748736 |
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| author | Katerina Jazbec Mojca Jež Klemen Žiberna Polonca Mali Živa Ramšak Urška Rahne Potokar Zdravko Kvrzić Maja Černilec Melita Gracar Marjana Šprohar Petra Jovanovič Sonja Vuletić Primož Rožman |
| author_facet | Katerina Jazbec Mojca Jež Klemen Žiberna Polonca Mali Živa Ramšak Urška Rahne Potokar Zdravko Kvrzić Maja Černilec Melita Gracar Marjana Šprohar Petra Jovanovič Sonja Vuletić Primož Rožman |
| author_sort | Katerina Jazbec |
| collection | DOAJ |
| description | Abstract Background Hyperimmune convalescent COVID-19 plasma (CCP) containing anti-SARS-CoV-2 neutralizing antibodies (NAbs) was proposed as a therapeutic option for patients early in the new coronavirus disease pandemic. The efficacy of this therapy depends on the quantity of neutralizing antibodies (NAbs) in the CCP units, with titers ≥ 1:160 being recommended. The standard neutralizing tests (NTs) used for determining appropriate CCP donors are technically demanding and expensive and take several days. We explored whether they could be replaced by high-throughput serology tests and a set of available clinical data. Methods Our study included 1302 CCP donors after PCR-confirmed COVID-19 infection. To predict donors with high NAb titers, we built four (4) multiple logistic regression models evaluating the relationships of demographic data, COVID-19 symptoms, results of various serological testing, the period between disease and donation, and COVID-19 vaccination status. Results The analysis of the four models showed that the chemiluminescent microparticle assay (CMIA) for the quantitative determination of IgG Abs to the RBD of the S1 subunit of the SARS-CoV-2 spike protein was enough to predict the CCP units with a high NAb titer. CCP donors with respective results > 850 BAU/ml SARS-CoV-2 IgG had a high probability of attaining sufficient NAb titers. Including additional variables such as donor demographics, clinical symptoms, or time of donation into a particular predictive model did not significantly increase its sensitivity and specificity. Conclusion A simple quantitative serological determination of anti-SARS-CoV-2 antibodies alone is satisfactory for recruiting CCP donors with high titer NAbs. |
| first_indexed | 2024-04-09T19:59:29Z |
| format | Article |
| id | doaj.art-4afa5b47a2a942329062cc8af271fcfb |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-09T19:59:29Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-4afa5b47a2a942329062cc8af271fcfb2023-04-03T05:16:23ZengBMCVirology Journal1743-422X2023-03-0120111010.1186/s12985-023-02007-0Simple prediction of COVID-19 convalescent plasma units with high levels of neutralization antibodiesKaterina Jazbec0Mojca Jež1Klemen Žiberna2Polonca Mali3Živa Ramšak4Urška Rahne Potokar5Zdravko Kvrzić6Maja Černilec7Melita Gracar8Marjana Šprohar9Petra Jovanovič10Sonja Vuletić11Primož Rožman12Blood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaNIB-National Institute of BiologyBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaBlood Transfusion Centre of SloveniaAbstract Background Hyperimmune convalescent COVID-19 plasma (CCP) containing anti-SARS-CoV-2 neutralizing antibodies (NAbs) was proposed as a therapeutic option for patients early in the new coronavirus disease pandemic. The efficacy of this therapy depends on the quantity of neutralizing antibodies (NAbs) in the CCP units, with titers ≥ 1:160 being recommended. The standard neutralizing tests (NTs) used for determining appropriate CCP donors are technically demanding and expensive and take several days. We explored whether they could be replaced by high-throughput serology tests and a set of available clinical data. Methods Our study included 1302 CCP donors after PCR-confirmed COVID-19 infection. To predict donors with high NAb titers, we built four (4) multiple logistic regression models evaluating the relationships of demographic data, COVID-19 symptoms, results of various serological testing, the period between disease and donation, and COVID-19 vaccination status. Results The analysis of the four models showed that the chemiluminescent microparticle assay (CMIA) for the quantitative determination of IgG Abs to the RBD of the S1 subunit of the SARS-CoV-2 spike protein was enough to predict the CCP units with a high NAb titer. CCP donors with respective results > 850 BAU/ml SARS-CoV-2 IgG had a high probability of attaining sufficient NAb titers. Including additional variables such as donor demographics, clinical symptoms, or time of donation into a particular predictive model did not significantly increase its sensitivity and specificity. Conclusion A simple quantitative serological determination of anti-SARS-CoV-2 antibodies alone is satisfactory for recruiting CCP donors with high titer NAbs.https://doi.org/10.1186/s12985-023-02007-0COVID-19 convalescent plasmaAnti-SARS-CoV-2 antibodiesNeutralization assayAntibody titerHyperimmune plasma donors |
| spellingShingle | Katerina Jazbec Mojca Jež Klemen Žiberna Polonca Mali Živa Ramšak Urška Rahne Potokar Zdravko Kvrzić Maja Černilec Melita Gracar Marjana Šprohar Petra Jovanovič Sonja Vuletić Primož Rožman Simple prediction of COVID-19 convalescent plasma units with high levels of neutralization antibodies Virology Journal COVID-19 convalescent plasma Anti-SARS-CoV-2 antibodies Neutralization assay Antibody titer Hyperimmune plasma donors |
| title | Simple prediction of COVID-19 convalescent plasma units with high levels of neutralization antibodies |
| title_full | Simple prediction of COVID-19 convalescent plasma units with high levels of neutralization antibodies |
| title_fullStr | Simple prediction of COVID-19 convalescent plasma units with high levels of neutralization antibodies |
| title_full_unstemmed | Simple prediction of COVID-19 convalescent plasma units with high levels of neutralization antibodies |
| title_short | Simple prediction of COVID-19 convalescent plasma units with high levels of neutralization antibodies |
| title_sort | simple prediction of covid 19 convalescent plasma units with high levels of neutralization antibodies |
| topic | COVID-19 convalescent plasma Anti-SARS-CoV-2 antibodies Neutralization assay Antibody titer Hyperimmune plasma donors |
| url | https://doi.org/10.1186/s12985-023-02007-0 |
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