Isolation in Natural Host Cell Lines of <i>Wolbachia</i> Strains <i>w</i>Pip from the Mosquito <i>Culex pipiens</i> and <i>w</i>Pap from the Sand Fly <i>Phlebotomus papatasi</i>
Endosymbiotic intracellular bacteria of the genus <i>Wolbachia</i> are harboured by many species of invertebrates. They display a wide range of developmental, metabolic and nutritional interactions with their hosts and may impact the transmission of arboviruses and protozoan parasites. &...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/10/871 |
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| author | Lesley Bell-Sakyi Alexandra Beliavskaia Catherine S. Hartley Laura Jones Lisa Luu Lee R. Haines James G. C. Hamilton Alistair C. Darby Benjamin L. Makepeace |
| author_facet | Lesley Bell-Sakyi Alexandra Beliavskaia Catherine S. Hartley Laura Jones Lisa Luu Lee R. Haines James G. C. Hamilton Alistair C. Darby Benjamin L. Makepeace |
| author_sort | Lesley Bell-Sakyi |
| collection | DOAJ |
| description | Endosymbiotic intracellular bacteria of the genus <i>Wolbachia</i> are harboured by many species of invertebrates. They display a wide range of developmental, metabolic and nutritional interactions with their hosts and may impact the transmission of arboviruses and protozoan parasites. <i>Wolbachia</i> have occasionally been isolated during insect cell line generation. Here, we report the isolation of two strains of <i>Wolbachia</i>, <i>w</i>Pip and <i>w</i>Pap, during cell line generation from their respective hosts, the mosquito <i>Culex pipiens</i> and the sand fly <i>Phlebotomus papatasi</i>. <i>w</i>Pip was pathogenic for both new <i>C. pipiens</i> cell lines, CPE/LULS50 and CLP/LULS56, requiring tetracycline treatment to rescue the lines. In contrast, <i>w</i>Pap was tolerated by the <i>P. papatasi</i> cell line PPL/LULS49, although tetracycline treatment was applied to generate a <i>Wolbachia</i>-free subline. Both <i>Wolbachia</i> strains were infective for a panel of heterologous insect and tick cell lines, including two novel lines generated from the sand fly <i>Lutzomyia longipalpis</i>, LLE/LULS45 and LLL/LULS52. In all cases, <i>w</i>Pip was more pathogenic for the host cells than <i>w</i>Pap. These newly isolated <i>Wolbachia</i> strains, and the novel mosquito and sand fly cell lines reported here, will add to the resources available for research on host–endosymbiont relationships, as well as on <i>C. pipiens</i>, <i>P. papatasi</i>, <i>L. longipalpis</i> and the pathogens that they transmit. |
| first_indexed | 2024-03-10T06:29:42Z |
| format | Article |
| id | doaj.art-4b06d2fa3b4046a6a46f31149d21b5ea |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T06:29:42Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-4b06d2fa3b4046a6a46f31149d21b5ea2023-11-22T18:38:49ZengMDPI AGInsects2075-44502021-09-01121087110.3390/insects12100871Isolation in Natural Host Cell Lines of <i>Wolbachia</i> Strains <i>w</i>Pip from the Mosquito <i>Culex pipiens</i> and <i>w</i>Pap from the Sand Fly <i>Phlebotomus papatasi</i>Lesley Bell-Sakyi0Alexandra Beliavskaia1Catherine S. Hartley2Laura Jones3Lisa Luu4Lee R. Haines5James G. C. Hamilton6Alistair C. Darby7Benjamin L. Makepeace8Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UKThe Pirbright Institute, Pirbright, Surrey GU24 0NF, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UKVector Biology Department, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UKDivision of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancashire LA1 4YG, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UKEndosymbiotic intracellular bacteria of the genus <i>Wolbachia</i> are harboured by many species of invertebrates. They display a wide range of developmental, metabolic and nutritional interactions with their hosts and may impact the transmission of arboviruses and protozoan parasites. <i>Wolbachia</i> have occasionally been isolated during insect cell line generation. Here, we report the isolation of two strains of <i>Wolbachia</i>, <i>w</i>Pip and <i>w</i>Pap, during cell line generation from their respective hosts, the mosquito <i>Culex pipiens</i> and the sand fly <i>Phlebotomus papatasi</i>. <i>w</i>Pip was pathogenic for both new <i>C. pipiens</i> cell lines, CPE/LULS50 and CLP/LULS56, requiring tetracycline treatment to rescue the lines. In contrast, <i>w</i>Pap was tolerated by the <i>P. papatasi</i> cell line PPL/LULS49, although tetracycline treatment was applied to generate a <i>Wolbachia</i>-free subline. Both <i>Wolbachia</i> strains were infective for a panel of heterologous insect and tick cell lines, including two novel lines generated from the sand fly <i>Lutzomyia longipalpis</i>, LLE/LULS45 and LLL/LULS52. In all cases, <i>w</i>Pip was more pathogenic for the host cells than <i>w</i>Pap. These newly isolated <i>Wolbachia</i> strains, and the novel mosquito and sand fly cell lines reported here, will add to the resources available for research on host–endosymbiont relationships, as well as on <i>C. pipiens</i>, <i>P. papatasi</i>, <i>L. longipalpis</i> and the pathogens that they transmit.https://www.mdpi.com/2075-4450/12/10/871mosquitosand flytick cell line<i>Wolbachia</i><i>Culex pipiens</i><i>Phlebotomus papatasi</i> |
| spellingShingle | Lesley Bell-Sakyi Alexandra Beliavskaia Catherine S. Hartley Laura Jones Lisa Luu Lee R. Haines James G. C. Hamilton Alistair C. Darby Benjamin L. Makepeace Isolation in Natural Host Cell Lines of <i>Wolbachia</i> Strains <i>w</i>Pip from the Mosquito <i>Culex pipiens</i> and <i>w</i>Pap from the Sand Fly <i>Phlebotomus papatasi</i> Insects mosquito sand fly tick cell line <i>Wolbachia</i> <i>Culex pipiens</i> <i>Phlebotomus papatasi</i> |
| title | Isolation in Natural Host Cell Lines of <i>Wolbachia</i> Strains <i>w</i>Pip from the Mosquito <i>Culex pipiens</i> and <i>w</i>Pap from the Sand Fly <i>Phlebotomus papatasi</i> |
| title_full | Isolation in Natural Host Cell Lines of <i>Wolbachia</i> Strains <i>w</i>Pip from the Mosquito <i>Culex pipiens</i> and <i>w</i>Pap from the Sand Fly <i>Phlebotomus papatasi</i> |
| title_fullStr | Isolation in Natural Host Cell Lines of <i>Wolbachia</i> Strains <i>w</i>Pip from the Mosquito <i>Culex pipiens</i> and <i>w</i>Pap from the Sand Fly <i>Phlebotomus papatasi</i> |
| title_full_unstemmed | Isolation in Natural Host Cell Lines of <i>Wolbachia</i> Strains <i>w</i>Pip from the Mosquito <i>Culex pipiens</i> and <i>w</i>Pap from the Sand Fly <i>Phlebotomus papatasi</i> |
| title_short | Isolation in Natural Host Cell Lines of <i>Wolbachia</i> Strains <i>w</i>Pip from the Mosquito <i>Culex pipiens</i> and <i>w</i>Pap from the Sand Fly <i>Phlebotomus papatasi</i> |
| title_sort | isolation in natural host cell lines of i wolbachia i strains i w i pip from the mosquito i culex pipiens i and i w i pap from the sand fly i phlebotomus papatasi i |
| topic | mosquito sand fly tick cell line <i>Wolbachia</i> <i>Culex pipiens</i> <i>Phlebotomus papatasi</i> |
| url | https://www.mdpi.com/2075-4450/12/10/871 |
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