PIM Kinases as Potential Biomarkers and Therapeutic Targets in Inflammatory Arthritides
The Proviral Integration site for the Moloney murine leukemia virus (PIM)-1 kinase and its family members (PIM-2 and PIM-3) regulate several cellular functions including survival, proliferation, and apoptosis. Recent studies showed their involvement in the pathogenesis of rheumatoid arthritis RA, wh...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/6/3123 |
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| author | Elisa Assirelli Jacopo Ciaffi Valentina Scorcu Susanna Naldi Veronica Brusi Luana Mancarella Lucia Lisi Federica Pignatti Francesco Ursini Simona Neri |
| author_facet | Elisa Assirelli Jacopo Ciaffi Valentina Scorcu Susanna Naldi Veronica Brusi Luana Mancarella Lucia Lisi Federica Pignatti Francesco Ursini Simona Neri |
| author_sort | Elisa Assirelli |
| collection | DOAJ |
| description | The Proviral Integration site for the Moloney murine leukemia virus (PIM)-1 kinase and its family members (PIM-2 and PIM-3) regulate several cellular functions including survival, proliferation, and apoptosis. Recent studies showed their involvement in the pathogenesis of rheumatoid arthritis RA, while no studies are available on psoriatic arthritis (PsA) and axial spondyloarthritis (axSpA). The main objective of this study is to assess the expression of PIM kinases in inflammatory arthritides, their correlation with proinflammatory cytokines, and their variation after treatment with biologic disease-modifying anti-rheumatic drugs or JAK inhibitors. We evaluated PIM-1, -2, and -3 expression at the gene and protein level, respectively, in the peripheral blood mononuclear cells and serum of patients with RA, PsA, axSpA, and healthy individuals (CTR). All the samples showed expression of PIM-1, -2, and -3 kinases both at the gene and protein level. PIM-1 was the most expressed protein, PIM-3 the least. PIM kinase levels differed between controls and disease groups, with reduced PIM-1 protein and increased PIM-3 protein in all disease samples compared to controls. No difference was found in the expression of these molecules between the three different pathologies. PIM levels were not modified after 6 months of therapy. In conclusion, our preliminary data suggest a deregulation of the PIM pathway in inflammatory arthritides. In-depth studies on the role of PIM kinases in this field are warranted. |
| first_indexed | 2024-04-24T18:12:17Z |
| format | Article |
| id | doaj.art-4b082b44ca084fdfa40753955188e159 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:12:17Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4b082b44ca084fdfa40753955188e1592024-03-27T13:45:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256312310.3390/ijms25063123PIM Kinases as Potential Biomarkers and Therapeutic Targets in Inflammatory ArthritidesElisa Assirelli0Jacopo Ciaffi1Valentina Scorcu2Susanna Naldi3Veronica Brusi4Luana Mancarella5Lucia Lisi6Federica Pignatti7Francesco Ursini8Simona Neri9Medicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyMedicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyThe Proviral Integration site for the Moloney murine leukemia virus (PIM)-1 kinase and its family members (PIM-2 and PIM-3) regulate several cellular functions including survival, proliferation, and apoptosis. Recent studies showed their involvement in the pathogenesis of rheumatoid arthritis RA, while no studies are available on psoriatic arthritis (PsA) and axial spondyloarthritis (axSpA). The main objective of this study is to assess the expression of PIM kinases in inflammatory arthritides, their correlation with proinflammatory cytokines, and their variation after treatment with biologic disease-modifying anti-rheumatic drugs or JAK inhibitors. We evaluated PIM-1, -2, and -3 expression at the gene and protein level, respectively, in the peripheral blood mononuclear cells and serum of patients with RA, PsA, axSpA, and healthy individuals (CTR). All the samples showed expression of PIM-1, -2, and -3 kinases both at the gene and protein level. PIM-1 was the most expressed protein, PIM-3 the least. PIM kinase levels differed between controls and disease groups, with reduced PIM-1 protein and increased PIM-3 protein in all disease samples compared to controls. No difference was found in the expression of these molecules between the three different pathologies. PIM levels were not modified after 6 months of therapy. In conclusion, our preliminary data suggest a deregulation of the PIM pathway in inflammatory arthritides. In-depth studies on the role of PIM kinases in this field are warranted.https://www.mdpi.com/1422-0067/25/6/3123rheumatoid arthritisaxial spondyloarthritispsoriatic arthritisPIM kinases |
| spellingShingle | Elisa Assirelli Jacopo Ciaffi Valentina Scorcu Susanna Naldi Veronica Brusi Luana Mancarella Lucia Lisi Federica Pignatti Francesco Ursini Simona Neri PIM Kinases as Potential Biomarkers and Therapeutic Targets in Inflammatory Arthritides International Journal of Molecular Sciences rheumatoid arthritis axial spondyloarthritis psoriatic arthritis PIM kinases |
| title | PIM Kinases as Potential Biomarkers and Therapeutic Targets in Inflammatory Arthritides |
| title_full | PIM Kinases as Potential Biomarkers and Therapeutic Targets in Inflammatory Arthritides |
| title_fullStr | PIM Kinases as Potential Biomarkers and Therapeutic Targets in Inflammatory Arthritides |
| title_full_unstemmed | PIM Kinases as Potential Biomarkers and Therapeutic Targets in Inflammatory Arthritides |
| title_short | PIM Kinases as Potential Biomarkers and Therapeutic Targets in Inflammatory Arthritides |
| title_sort | pim kinases as potential biomarkers and therapeutic targets in inflammatory arthritides |
| topic | rheumatoid arthritis axial spondyloarthritis psoriatic arthritis PIM kinases |
| url | https://www.mdpi.com/1422-0067/25/6/3123 |
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