Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtype
<p>Abstract</p> <p>Background</p> <p>Pseudomyxoma peritonei (PMP) is a rare malignant disease, most commonly originating from appendiceal lesions and characterized by accumulation of mucinous tumor tissue in the peritoneal cavity. Since the disease is infrequent, the ta...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-06-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/7/116 |
| _version_ | 1828254593398603776 |
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| author | Wiig Johan N Sørensen Olaf Halvorsen Thomas Reed Wenche Flatmark Kjersti Larsen Stein G Fodstad Øystein Giercksky Karl-Erik |
| author_facet | Wiig Johan N Sørensen Olaf Halvorsen Thomas Reed Wenche Flatmark Kjersti Larsen Stein G Fodstad Øystein Giercksky Karl-Erik |
| author_sort | Wiig Johan N |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Pseudomyxoma peritonei (PMP) is a rare malignant disease, most commonly originating from appendiceal lesions and characterized by accumulation of mucinous tumor tissue in the peritoneal cavity. Since the disease is infrequent, the task of carrying out studies of treatment efficacy and disease biology in the clinical setting is challenging, warranting the development of relevant <it>in vitro </it>and <it>in vivo </it>PMP models.</p> <p>Methods</p> <p>Human tumor tissue was implanted in the peritoneal cavity of nude mice to establish two orthotopic models exhibiting noninvasive intraperitoneal growth without metastasis development.</p> <p>Results</p> <p>Xenograft tissues have retained essential properties of the original human tumors, such as macro- and microscopic growth patterns, mucin production as well as expression of carcinoembryonal antigen, cytokeratins 20 and 7 and the proliferation marker pKi67. Upon microscopic examination, the human tumors were categorized as the PMCA-I (peritoneal mucinous carcinomatosis of intermediate features) subtype, which was conserved through 14 examined passages in mice, for the first time modeling this particular histopathologic category.</p> <p>Conclusion</p> <p>In conclusion, two novel orthotopic models of human PMP have been established that consistently portray a distinct histopathologic subtype and reflect essential human tumor properties. Xenografts can easily and reproducibly be transferred to new generations of mice with acceptable passage periods, rendering the models as attractive tools for further studies of PMP biology and treatment.</p> |
| first_indexed | 2024-04-13T02:01:33Z |
| format | Article |
| id | doaj.art-4b11bb39e2ed4c64af5705bf787c3bf0 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-13T02:01:33Z |
| publishDate | 2007-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-4b11bb39e2ed4c64af5705bf787c3bf02022-12-22T03:07:37ZengBMCBMC Cancer1471-24072007-06-017111610.1186/1471-2407-7-116Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtypeWiig Johan NSørensen OlafHalvorsen ThomasReed WencheFlatmark KjerstiLarsen Stein GFodstad ØysteinGiercksky Karl-Erik<p>Abstract</p> <p>Background</p> <p>Pseudomyxoma peritonei (PMP) is a rare malignant disease, most commonly originating from appendiceal lesions and characterized by accumulation of mucinous tumor tissue in the peritoneal cavity. Since the disease is infrequent, the task of carrying out studies of treatment efficacy and disease biology in the clinical setting is challenging, warranting the development of relevant <it>in vitro </it>and <it>in vivo </it>PMP models.</p> <p>Methods</p> <p>Human tumor tissue was implanted in the peritoneal cavity of nude mice to establish two orthotopic models exhibiting noninvasive intraperitoneal growth without metastasis development.</p> <p>Results</p> <p>Xenograft tissues have retained essential properties of the original human tumors, such as macro- and microscopic growth patterns, mucin production as well as expression of carcinoembryonal antigen, cytokeratins 20 and 7 and the proliferation marker pKi67. Upon microscopic examination, the human tumors were categorized as the PMCA-I (peritoneal mucinous carcinomatosis of intermediate features) subtype, which was conserved through 14 examined passages in mice, for the first time modeling this particular histopathologic category.</p> <p>Conclusion</p> <p>In conclusion, two novel orthotopic models of human PMP have been established that consistently portray a distinct histopathologic subtype and reflect essential human tumor properties. Xenografts can easily and reproducibly be transferred to new generations of mice with acceptable passage periods, rendering the models as attractive tools for further studies of PMP biology and treatment.</p>http://www.biomedcentral.com/1471-2407/7/116 |
| spellingShingle | Wiig Johan N Sørensen Olaf Halvorsen Thomas Reed Wenche Flatmark Kjersti Larsen Stein G Fodstad Øystein Giercksky Karl-Erik Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtype BMC Cancer |
| title | Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtype |
| title_full | Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtype |
| title_fullStr | Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtype |
| title_full_unstemmed | Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtype |
| title_short | Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtype |
| title_sort | pseudomyxoma peritonei two novel orthotopic mouse models portray the pmca i histopathologic subtype |
| url | http://www.biomedcentral.com/1471-2407/7/116 |
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