Bioconversion of biodiesel refinery waste in the bioemulsifier by <it>Trichosporon mycotoxinivorans</it> CLA2
<p>Abstract</p> <p>Background</p> <p>The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes.</p> <p&g...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-05-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://www.biotechnologyforbiofuels.com/content/5/1/29 |
| _version_ | 1828806465573355520 |
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| author | de Souza Monteiro Andrea Domingues Vitor Souza Marcus VD Lula Ivana Gonçalves Daniel de Siqueira Ezequias Pessoa dos Santos Vera |
| author_facet | de Souza Monteiro Andrea Domingues Vitor Souza Marcus VD Lula Ivana Gonçalves Daniel de Siqueira Ezequias Pessoa dos Santos Vera |
| author_sort | de Souza Monteiro Andrea |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes.</p> <p>Results</p> <p><it>Trichosporon mycotoxinivorans</it> CLA2 is bioemulsifier-producing yeast strain isolated from effluents of the dairy industry, with ability to emulsify different hydrophobic substrates. Bioemulsifier production (mg/L) and the emulsifying activity (E<sub>24</sub>) of this strain were optimized by response surface methodology using mineral minimal medium containing refinery waste as the carbon source, which consisted of diatomaceous earth impregnated with esters from filters used in biodiesel purification. The highest bioemulsifier production occurred in mineral minimal medium containing 75 g/L biodiesel residue and 5 g/L ammonium sulfate. The highest emulsifying activity was obtained in medium containing 58 g/L biodiesel refinery residue and 4.6 g/L ammonium sulfate, and under these conditions, the model estimated an emulsifying activity of 85%. Gas chromatography and mass spectrometry analysis suggested a bioemulsifier molecule consisting of monosaccharides, predominantly xylose and mannose, and a long chain aliphatic groups composed of octadecanoic acid and hexadecanoic acid at concentrations of 48.01% and 43.16%, respectively. The carbohydrate composition as determined by GC-MS of their alditol acetate derivatives showed a larger ratio of xylose (49.27%), mannose (39.91%), and glucose (10.81%). <sup>1</sup> H NMR spectra confirmed by COSY suggested high molecular weight, polymeric pattern, presence of monosaccharide’s and long chain aliphatic groups in the bioemulsifier molecule.</p> <p>Conclusions</p> <p>The biodiesel residue is an economical substrate, therefore seems to be very promising for the low-cost production of active emulsifiers in the emulsification of aromatics, aliphatic hydrocarbons, and kerosene.</p> |
| first_indexed | 2024-12-12T08:10:03Z |
| format | Article |
| id | doaj.art-4b14ccc446cc429bbd20357fa4be0e15 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T08:10:03Z |
| publishDate | 2012-05-01 |
| publisher | BMC |
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| series | Biotechnology for Biofuels |
| spelling | doaj.art-4b14ccc446cc429bbd20357fa4be0e152022-12-22T00:31:48ZengBMCBiotechnology for Biofuels1754-68342012-05-01512910.1186/1754-6834-5-29Bioconversion of biodiesel refinery waste in the bioemulsifier by <it>Trichosporon mycotoxinivorans</it> CLA2de Souza Monteiro AndreaDomingues VitorSouza Marcus VDLula IvanaGonçalves Danielde Siqueira Ezequias Pessoados Santos Vera<p>Abstract</p> <p>Background</p> <p>The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes.</p> <p>Results</p> <p><it>Trichosporon mycotoxinivorans</it> CLA2 is bioemulsifier-producing yeast strain isolated from effluents of the dairy industry, with ability to emulsify different hydrophobic substrates. Bioemulsifier production (mg/L) and the emulsifying activity (E<sub>24</sub>) of this strain were optimized by response surface methodology using mineral minimal medium containing refinery waste as the carbon source, which consisted of diatomaceous earth impregnated with esters from filters used in biodiesel purification. The highest bioemulsifier production occurred in mineral minimal medium containing 75 g/L biodiesel residue and 5 g/L ammonium sulfate. The highest emulsifying activity was obtained in medium containing 58 g/L biodiesel refinery residue and 4.6 g/L ammonium sulfate, and under these conditions, the model estimated an emulsifying activity of 85%. Gas chromatography and mass spectrometry analysis suggested a bioemulsifier molecule consisting of monosaccharides, predominantly xylose and mannose, and a long chain aliphatic groups composed of octadecanoic acid and hexadecanoic acid at concentrations of 48.01% and 43.16%, respectively. The carbohydrate composition as determined by GC-MS of their alditol acetate derivatives showed a larger ratio of xylose (49.27%), mannose (39.91%), and glucose (10.81%). <sup>1</sup> H NMR spectra confirmed by COSY suggested high molecular weight, polymeric pattern, presence of monosaccharide’s and long chain aliphatic groups in the bioemulsifier molecule.</p> <p>Conclusions</p> <p>The biodiesel residue is an economical substrate, therefore seems to be very promising for the low-cost production of active emulsifiers in the emulsification of aromatics, aliphatic hydrocarbons, and kerosene.</p>http://www.biotechnologyforbiofuels.com/content/5/1/29BioemulsifierYeastsResponse surface methodologyBiodiesel residueDiatomaceous earth |
| spellingShingle | de Souza Monteiro Andrea Domingues Vitor Souza Marcus VD Lula Ivana Gonçalves Daniel de Siqueira Ezequias Pessoa dos Santos Vera Bioconversion of biodiesel refinery waste in the bioemulsifier by <it>Trichosporon mycotoxinivorans</it> CLA2 Biotechnology for Biofuels Bioemulsifier Yeasts Response surface methodology Biodiesel residue Diatomaceous earth |
| title | Bioconversion of biodiesel refinery waste in the bioemulsifier by <it>Trichosporon mycotoxinivorans</it> CLA2 |
| title_full | Bioconversion of biodiesel refinery waste in the bioemulsifier by <it>Trichosporon mycotoxinivorans</it> CLA2 |
| title_fullStr | Bioconversion of biodiesel refinery waste in the bioemulsifier by <it>Trichosporon mycotoxinivorans</it> CLA2 |
| title_full_unstemmed | Bioconversion of biodiesel refinery waste in the bioemulsifier by <it>Trichosporon mycotoxinivorans</it> CLA2 |
| title_short | Bioconversion of biodiesel refinery waste in the bioemulsifier by <it>Trichosporon mycotoxinivorans</it> CLA2 |
| title_sort | bioconversion of biodiesel refinery waste in the bioemulsifier by it trichosporon mycotoxinivorans it cla2 |
| topic | Bioemulsifier Yeasts Response surface methodology Biodiesel residue Diatomaceous earth |
| url | http://www.biotechnologyforbiofuels.com/content/5/1/29 |
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