Rifamycin resistance, rpoB gene mutation and clinical outcomes of Staphylococcus species isolates from prosthetic joint infections in Republic of Korea
ABSTRACT: Objectives: We conducted an in vitro investigation of the activity of rifamycins against planktonic and biofilm states of Staphylococcus aureus and Staphylococcus epidermidis isolates from patients with prosthetic joint infections (PJIs), characterised their rpoB gene mutations, and analy...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716521002769 |
| _version_ | 1818358403954638848 |
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| author | Yunjung Lee Soo Sung Kim Su-Mi Choi Chan-Joong Bae Tae-Hoon Oh Seong Eun Kim Uh Jin Kim Seung-Ji Kang Sook-In Jung Kyung-Hwa Park |
| author_facet | Yunjung Lee Soo Sung Kim Su-Mi Choi Chan-Joong Bae Tae-Hoon Oh Seong Eun Kim Uh Jin Kim Seung-Ji Kang Sook-In Jung Kyung-Hwa Park |
| author_sort | Yunjung Lee |
| collection | DOAJ |
| description | ABSTRACT: Objectives: We conducted an in vitro investigation of the activity of rifamycins against planktonic and biofilm states of Staphylococcus aureus and Staphylococcus epidermidis isolates from patients with prosthetic joint infections (PJIs), characterised their rpoB gene mutations, and analysed the clinical outcomes of rifampicin-resistant isolates. Methods: A total of 110 staphylococcal isolates were collected from patients with PJI. Antimicrobials tested using the broth microdilution method included rifampicin, rifabutin, rifapentine and rifaximin. We evaluated rpoB gene mutations to identify rifampicin resistance mechanisms. Clinical outcomes were assessed in rifampicin-resistant isolates. Results: The 110 staphylococcal isolates included 85 S. aureus (55% methicillin-resistant) and 25 S. epidermidis (100% methicillin-resistant). Seven S. aureus isolates and two S. epidermidis isolates were resistant to rifampicin [minimum inhibitory concentration (MIC) ≥2 μg/mL] and these isolates exhibited rpoB gene mutations. Among the 78 rifampicin-susceptible S. aureus isolates and 23 S. epidermidis isolates, 76 S. aureus isolates (97.4%) and all S. epidermidis isolates (100%) were highly susceptible (MIC ≤ 0.06 μg/mL) to other rifamycins. The minimum biofilm bactericidal concentrations for ≥50% of isolates (MBBC50) to rifampicin, rifabutin, rifapentine and rifaximin were 4, 1, 2 and 4 μg/mL for S. aureus and 1, 0.125, 0.25 and 0.5 μg/mL for S. epidermidis, respectively, among rifampicin-susceptible isolates. Among nine patients bearing rifampicin-resistant isolates, only three (33%) had successful outcomes. Conclusion: Rifamycins other than rifampicin show promising antistaphylococcal activity, including antibiofilm activity. Rifamycin-resistant staphylococci exhibit rpoB gene mutations. |
| first_indexed | 2024-12-13T20:28:27Z |
| format | Article |
| id | doaj.art-4b1600495a594364af3db87644deb57b |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-13T20:28:27Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-4b1600495a594364af3db87644deb57b2022-12-21T23:32:29ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-03-01284348Rifamycin resistance, rpoB gene mutation and clinical outcomes of Staphylococcus species isolates from prosthetic joint infections in Republic of KoreaYunjung Lee0Soo Sung Kim1Su-Mi Choi2Chan-Joong Bae3Tae-Hoon Oh4Seong Eun Kim5Uh Jin Kim6Seung-Ji Kang7Sook-In Jung8Kyung-Hwa Park9Department of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of Korea; Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of Korea; Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of Korea; Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of Korea; Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Republic of KoreaDepartment of Infectious Diseases, Chonnam National University Hospital, Gwangju, Republic of Korea; Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Republic of Korea; Corresponding author. Mailing address: Department of Infectious Diseases, Chonnam National University Medical School, 42 Jebong-ro, Dong-gu, Gwangju 501-757, Republic of Korea. Tel.: +82 62 220 6296; fax: +82 62 225 8578.ABSTRACT: Objectives: We conducted an in vitro investigation of the activity of rifamycins against planktonic and biofilm states of Staphylococcus aureus and Staphylococcus epidermidis isolates from patients with prosthetic joint infections (PJIs), characterised their rpoB gene mutations, and analysed the clinical outcomes of rifampicin-resistant isolates. Methods: A total of 110 staphylococcal isolates were collected from patients with PJI. Antimicrobials tested using the broth microdilution method included rifampicin, rifabutin, rifapentine and rifaximin. We evaluated rpoB gene mutations to identify rifampicin resistance mechanisms. Clinical outcomes were assessed in rifampicin-resistant isolates. Results: The 110 staphylococcal isolates included 85 S. aureus (55% methicillin-resistant) and 25 S. epidermidis (100% methicillin-resistant). Seven S. aureus isolates and two S. epidermidis isolates were resistant to rifampicin [minimum inhibitory concentration (MIC) ≥2 μg/mL] and these isolates exhibited rpoB gene mutations. Among the 78 rifampicin-susceptible S. aureus isolates and 23 S. epidermidis isolates, 76 S. aureus isolates (97.4%) and all S. epidermidis isolates (100%) were highly susceptible (MIC ≤ 0.06 μg/mL) to other rifamycins. The minimum biofilm bactericidal concentrations for ≥50% of isolates (MBBC50) to rifampicin, rifabutin, rifapentine and rifaximin were 4, 1, 2 and 4 μg/mL for S. aureus and 1, 0.125, 0.25 and 0.5 μg/mL for S. epidermidis, respectively, among rifampicin-susceptible isolates. Among nine patients bearing rifampicin-resistant isolates, only three (33%) had successful outcomes. Conclusion: Rifamycins other than rifampicin show promising antistaphylococcal activity, including antibiofilm activity. Rifamycin-resistant staphylococci exhibit rpoB gene mutations.http://www.sciencedirect.com/science/article/pii/S2213716521002769Prosthetic joint infectionStaphylococcus aureusStaphylococcus epidermidisRifamycinsrpoB geneSusceptibility |
| spellingShingle | Yunjung Lee Soo Sung Kim Su-Mi Choi Chan-Joong Bae Tae-Hoon Oh Seong Eun Kim Uh Jin Kim Seung-Ji Kang Sook-In Jung Kyung-Hwa Park Rifamycin resistance, rpoB gene mutation and clinical outcomes of Staphylococcus species isolates from prosthetic joint infections in Republic of Korea Journal of Global Antimicrobial Resistance Prosthetic joint infection Staphylococcus aureus Staphylococcus epidermidis Rifamycins rpoB gene Susceptibility |
| title | Rifamycin resistance, rpoB gene mutation and clinical outcomes of Staphylococcus species isolates from prosthetic joint infections in Republic of Korea |
| title_full | Rifamycin resistance, rpoB gene mutation and clinical outcomes of Staphylococcus species isolates from prosthetic joint infections in Republic of Korea |
| title_fullStr | Rifamycin resistance, rpoB gene mutation and clinical outcomes of Staphylococcus species isolates from prosthetic joint infections in Republic of Korea |
| title_full_unstemmed | Rifamycin resistance, rpoB gene mutation and clinical outcomes of Staphylococcus species isolates from prosthetic joint infections in Republic of Korea |
| title_short | Rifamycin resistance, rpoB gene mutation and clinical outcomes of Staphylococcus species isolates from prosthetic joint infections in Republic of Korea |
| title_sort | rifamycin resistance rpob gene mutation and clinical outcomes of staphylococcus species isolates from prosthetic joint infections in republic of korea |
| topic | Prosthetic joint infection Staphylococcus aureus Staphylococcus epidermidis Rifamycins rpoB gene Susceptibility |
| url | http://www.sciencedirect.com/science/article/pii/S2213716521002769 |
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