Mycobacterium avium Modulates the Protective Immune Response in Canine Peripheral Blood Mononuclear Cells
Mycobacterium avium, an opportunistic intracellular pathogen, is a member of the non-tuberculous mycobacteria species. M. avium causes respiratory disease in immunosuppressed individuals and a wide range of animals, including companion dogs and cats. In particular, the number of infected companion d...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2020.609712/full |
| _version_ | 1818960505668108288 |
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| author | Suji Kim Suji Kim Hyun-Eui Park Woo Bin Park Seo Yihl Kim Hong-Tae Park Han Sang Yoo Han Sang Yoo Han Sang Yoo |
| author_facet | Suji Kim Suji Kim Hyun-Eui Park Woo Bin Park Seo Yihl Kim Hong-Tae Park Han Sang Yoo Han Sang Yoo Han Sang Yoo |
| author_sort | Suji Kim |
| collection | DOAJ |
| description | Mycobacterium avium, an opportunistic intracellular pathogen, is a member of the non-tuberculous mycobacteria species. M. avium causes respiratory disease in immunosuppressed individuals and a wide range of animals, including companion dogs and cats. In particular, the number of infected companion dogs has increased, although the underlying mechanism of M. avium pathogenesis in dogs has not been studied. Therefore, in the present study, the host immune response against M. avium in dogs was investigated by transcriptome analysis of canine peripheral blood mononuclear cells. M. avium was shown to induce different immune responses in canine peripheral blood mononuclear cells at different time points after infection. The expression of Th1-associated genes occurred early during M. avium infection, while that of Th17-associated genes increased after 12 h. In addition, the expression of apoptosis-related genes decreased and the abundance of intracellular M. avium increased in monocyte-derived macrophages after infection for 24 h. These results reveal the M. avium induces Th17 immune response and avoids apoptosis in infected canine cells. As the number of M. avium infection cases increases, the results of the present study will contribute to a better understanding of host immune responses to M. avium infection in companion dogs. |
| first_indexed | 2024-12-20T11:58:36Z |
| format | Article |
| id | doaj.art-4b1b5d590d7a415686edb96ad04ebd8e |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-20T11:58:36Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-4b1b5d590d7a415686edb96ad04ebd8e2022-12-21T19:41:36ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-01-011010.3389/fcimb.2020.609712609712Mycobacterium avium Modulates the Protective Immune Response in Canine Peripheral Blood Mononuclear CellsSuji Kim0Suji Kim1Hyun-Eui Park2Woo Bin Park3Seo Yihl Kim4Hong-Tae Park5Han Sang Yoo6Han Sang Yoo7Han Sang Yoo8Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, South KoreaBK21 FOUR Future Veterinary Medicine Leading Education and Research Center, Seoul National University, Seoul, South KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju, South KoreaDepartment of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, South KoreaDepartment of Veterinary Physiology, College of Veterinary Medicine, Seoul National University, Seoul, South KoreaDepartment of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, South KoreaDepartment of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, South KoreaBK21 FOUR Future Veterinary Medicine Leading Education and Research Center, Seoul National University, Seoul, South KoreaBio-MAX/N-Bio Institute, Seoul National University, Seoul, South KoreaMycobacterium avium, an opportunistic intracellular pathogen, is a member of the non-tuberculous mycobacteria species. M. avium causes respiratory disease in immunosuppressed individuals and a wide range of animals, including companion dogs and cats. In particular, the number of infected companion dogs has increased, although the underlying mechanism of M. avium pathogenesis in dogs has not been studied. Therefore, in the present study, the host immune response against M. avium in dogs was investigated by transcriptome analysis of canine peripheral blood mononuclear cells. M. avium was shown to induce different immune responses in canine peripheral blood mononuclear cells at different time points after infection. The expression of Th1-associated genes occurred early during M. avium infection, while that of Th17-associated genes increased after 12 h. In addition, the expression of apoptosis-related genes decreased and the abundance of intracellular M. avium increased in monocyte-derived macrophages after infection for 24 h. These results reveal the M. avium induces Th17 immune response and avoids apoptosis in infected canine cells. As the number of M. avium infection cases increases, the results of the present study will contribute to a better understanding of host immune responses to M. avium infection in companion dogs.https://www.frontiersin.org/articles/10.3389/fcimb.2020.609712/fullMycobacterium aviumhost immune responseTh17apoptosisdog |
| spellingShingle | Suji Kim Suji Kim Hyun-Eui Park Woo Bin Park Seo Yihl Kim Hong-Tae Park Han Sang Yoo Han Sang Yoo Han Sang Yoo Mycobacterium avium Modulates the Protective Immune Response in Canine Peripheral Blood Mononuclear Cells Frontiers in Cellular and Infection Microbiology Mycobacterium avium host immune response Th17 apoptosis dog |
| title | Mycobacterium avium Modulates the Protective Immune Response in Canine Peripheral Blood Mononuclear Cells |
| title_full | Mycobacterium avium Modulates the Protective Immune Response in Canine Peripheral Blood Mononuclear Cells |
| title_fullStr | Mycobacterium avium Modulates the Protective Immune Response in Canine Peripheral Blood Mononuclear Cells |
| title_full_unstemmed | Mycobacterium avium Modulates the Protective Immune Response in Canine Peripheral Blood Mononuclear Cells |
| title_short | Mycobacterium avium Modulates the Protective Immune Response in Canine Peripheral Blood Mononuclear Cells |
| title_sort | mycobacterium avium modulates the protective immune response in canine peripheral blood mononuclear cells |
| topic | Mycobacterium avium host immune response Th17 apoptosis dog |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2020.609712/full |
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