Ascorbic acid for the healing of skin wounds in rats
BACKGROUND: Healing is a complex process that involves cellular and biochemical events. Several medicines have been used in order to shorten healing time and avoid aesthetic damage. OBJECTIVE: to verify the topical effect of ascorbic acid for the healing of rats' skin wounds through the number...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842009000500026&lng=en&tlng=en |
| _version_ | 1819179826204901376 |
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| author | CC. Lima APC. Pereira JRF. Silva LS. Oliveira MCC. Resck CO. Grechi MTCP. Bernardes FMP. Olímpio AMM. Santos EK. Incerpi JAD. Garcia |
| author_facet | CC. Lima APC. Pereira JRF. Silva LS. Oliveira MCC. Resck CO. Grechi MTCP. Bernardes FMP. Olímpio AMM. Santos EK. Incerpi JAD. Garcia |
| author_sort | CC. Lima |
| collection | DOAJ |
| description | BACKGROUND: Healing is a complex process that involves cellular and biochemical events. Several medicines have been used in order to shorten healing time and avoid aesthetic damage. OBJECTIVE: to verify the topical effect of ascorbic acid for the healing of rats' skin wounds through the number of macrophages, new vessels and fibroblast verifications in the experimental period; and analyse the thickness and the collagen fibre organization in the injured tissue. METHODS: Male Rattus norvegicus weighing 270 ± 30 g were used. After thionembutal anesthesia, 15 mm transversal incisions were made in the animals' cervical backs. They were divided into two groups: Control Group (CG, n = 12) - skin wound cleaned with water and soap daily; Treated Group (TG, n = 12) - skin wound cleaned daily and treated with ascorbic acid cream (10%). Samples of skin were collected on the 3rd, 7th and 14th days. The sections were stained with hematoxylin-eosin and picrosirius red for morphologic analysis. The images were obtained and analysed by a Digital Analyser System. RESULTS: The ascorbic acid acted on every stage of the healing process. It reduced the number of macrophages, increased the proliferation of fibroblasts and new vessels, and stimulated the synthesis of thicker and more organized collagen fibres in the wounds when compared to CG. CONCLUSION: Ascorbic acid was shown to have anti-inflammatory and healing effects, guaranteeing a suiTable environment and conditions for faster skin repair. |
| first_indexed | 2024-12-22T22:04:37Z |
| format | Article |
| id | doaj.art-4b1cc19862034dc2890af1f982733b5f |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-22T22:04:37Z |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-4b1cc19862034dc2890af1f982733b5f2022-12-21T18:11:02ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43756941195120110.1590/S1519-69842009000500026S1519-69842009000500026Ascorbic acid for the healing of skin wounds in ratsCC. Lima0APC. Pereira1JRF. Silva2LS. OliveiraMCC. Resck3CO. Grechi4MTCP. Bernardes5FMP. Olímpio6AMM. Santos7EK. Incerpi8JAD. Garcia9Universidade José do Rosário VellanoUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoEscola Estadual Judithy ViannaUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoUniversidade de Três CoraçõesUniversidade José do Rosário VellanoBACKGROUND: Healing is a complex process that involves cellular and biochemical events. Several medicines have been used in order to shorten healing time and avoid aesthetic damage. OBJECTIVE: to verify the topical effect of ascorbic acid for the healing of rats' skin wounds through the number of macrophages, new vessels and fibroblast verifications in the experimental period; and analyse the thickness and the collagen fibre organization in the injured tissue. METHODS: Male Rattus norvegicus weighing 270 ± 30 g were used. After thionembutal anesthesia, 15 mm transversal incisions were made in the animals' cervical backs. They were divided into two groups: Control Group (CG, n = 12) - skin wound cleaned with water and soap daily; Treated Group (TG, n = 12) - skin wound cleaned daily and treated with ascorbic acid cream (10%). Samples of skin were collected on the 3rd, 7th and 14th days. The sections were stained with hematoxylin-eosin and picrosirius red for morphologic analysis. The images were obtained and analysed by a Digital Analyser System. RESULTS: The ascorbic acid acted on every stage of the healing process. It reduced the number of macrophages, increased the proliferation of fibroblasts and new vessels, and stimulated the synthesis of thicker and more organized collagen fibres in the wounds when compared to CG. CONCLUSION: Ascorbic acid was shown to have anti-inflammatory and healing effects, guaranteeing a suiTable environment and conditions for faster skin repair.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842009000500026&lng=en&tlng=enwound healingascorbic acidcollagenfibroblastsskin |
| spellingShingle | CC. Lima APC. Pereira JRF. Silva LS. Oliveira MCC. Resck CO. Grechi MTCP. Bernardes FMP. Olímpio AMM. Santos EK. Incerpi JAD. Garcia Ascorbic acid for the healing of skin wounds in rats Brazilian Journal of Biology wound healing ascorbic acid collagen fibroblasts skin |
| title | Ascorbic acid for the healing of skin wounds in rats |
| title_full | Ascorbic acid for the healing of skin wounds in rats |
| title_fullStr | Ascorbic acid for the healing of skin wounds in rats |
| title_full_unstemmed | Ascorbic acid for the healing of skin wounds in rats |
| title_short | Ascorbic acid for the healing of skin wounds in rats |
| title_sort | ascorbic acid for the healing of skin wounds in rats |
| topic | wound healing ascorbic acid collagen fibroblasts skin |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842009000500026&lng=en&tlng=en |
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