Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect
Effects of conservative treatment on uterine blood flow and morphometric findings are still unknown in bitches. Thus, this study aimed to compare uterine changes of pyometra bitches subjected to distinct modes of treatment. Pyometra bitches were assigned to: OHE (ovariohysterectomy immediately after...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Bioscientifica
2021-04-01
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| Series: | Reproduction and Fertility |
| Subjects: | |
| Online Access: | https://raf.bioscientifica.com/view/journals/raf/2/2/RAF-20-0020.xml |
| _version_ | 1818456214467510272 |
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| author | Roberto Rodrigues da Rosa Filho Maíra Morales Brito Thaís Gomes Faustino Leticia Lima de Almeida Verônica Correia Manoel Bruno Cogliati Camila Infantosi Vannucchi |
| author_facet | Roberto Rodrigues da Rosa Filho Maíra Morales Brito Thaís Gomes Faustino Leticia Lima de Almeida Verônica Correia Manoel Bruno Cogliati Camila Infantosi Vannucchi |
| author_sort | Roberto Rodrigues da Rosa Filho |
| collection | DOAJ |
| description | Effects of conservative treatment on uterine blood flow and morphometric findings are still unknown in bitches. Thus, this study aimed to compare uterine changes of pyometra bitches subjected to distinct modes of treatment. Pyometra bitches were assigned to: OHE (ovariohysterectomy immediately after diagnosis), Aglepristone (days 1, 2 and 8) and Associative (aglepristone treatment coupled with cloprostenol for 7 days) groups. After 9 days, bitches were ovariohysterectomized. Before surgery, uterine area was measured ultrasonographically and the uterine artery Doppler velocimetry analyzed blood flow velocity and indexes. Uterine horns were classified according to resistance index (RI) as more compromised and less compromised. Endometrial vasculature was quantitatively evaluated by color flow Doppler. Blood samples were collected to determine nitric oxide (NO) concentrations. Histological uterine structures were quantified by stereology and VEGF-A (vascular endothelial growth factor) and eNOS were (endothelial nitric oxide synthase) immunohistochemically analyzed. Aglepristone and Associative groups had lower uterine area and vascularization, and higher blood flow velocity and indexes compared to OHE group. Less compromised horn of Associative group had higher blood flow velocity compared to OHE group. Aglepristone group presented lower inflammatory infiltrate and larger uterine stroma. Associative group had lower volume density and absolute surface of endometrial cysts and lower VEGF-A expression for glandular epithelium and stromal cells. Blood NO and e-NOS immunostaining were not different among groups. In conclusion, association between aglepristone and prostaglandin is more effective in decreasing uterine vascularization and modulating uterine blood flow. Moreover, associative therapy promotes marked morphological changes. |
| first_indexed | 2024-12-14T22:23:07Z |
| format | Article |
| id | doaj.art-4b1e266be08a4b1db8fffaf1d78925e4 |
| institution | Directory Open Access Journal |
| issn | 2633-8386 2633-8386 |
| language | English |
| last_indexed | 2024-12-14T22:23:07Z |
| publishDate | 2021-04-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj.art-4b1e266be08a4b1db8fffaf1d78925e42022-12-21T22:45:27ZengBioscientificaReproduction and Fertility2633-83862633-83862021-04-012295105https://doi.org/10.1530/RAF-20-0020Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effectRoberto Rodrigues da Rosa Filho0Maíra Morales Brito1Thaís Gomes Faustino2Leticia Lima de Almeida3Verônica Correia Manoel4Bruno Cogliati5Camila Infantosi Vannucchi6Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, BrazilDepartment of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, BrazilDepartment of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, BrazilDepartment of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, BrazilDepartment of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, BrazilDepartment of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, BrazilDepartment of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, BrazilEffects of conservative treatment on uterine blood flow and morphometric findings are still unknown in bitches. Thus, this study aimed to compare uterine changes of pyometra bitches subjected to distinct modes of treatment. Pyometra bitches were assigned to: OHE (ovariohysterectomy immediately after diagnosis), Aglepristone (days 1, 2 and 8) and Associative (aglepristone treatment coupled with cloprostenol for 7 days) groups. After 9 days, bitches were ovariohysterectomized. Before surgery, uterine area was measured ultrasonographically and the uterine artery Doppler velocimetry analyzed blood flow velocity and indexes. Uterine horns were classified according to resistance index (RI) as more compromised and less compromised. Endometrial vasculature was quantitatively evaluated by color flow Doppler. Blood samples were collected to determine nitric oxide (NO) concentrations. Histological uterine structures were quantified by stereology and VEGF-A (vascular endothelial growth factor) and eNOS were (endothelial nitric oxide synthase) immunohistochemically analyzed. Aglepristone and Associative groups had lower uterine area and vascularization, and higher blood flow velocity and indexes compared to OHE group. Less compromised horn of Associative group had higher blood flow velocity compared to OHE group. Aglepristone group presented lower inflammatory infiltrate and larger uterine stroma. Associative group had lower volume density and absolute surface of endometrial cysts and lower VEGF-A expression for glandular epithelium and stromal cells. Blood NO and e-NOS immunostaining were not different among groups. In conclusion, association between aglepristone and prostaglandin is more effective in decreasing uterine vascularization and modulating uterine blood flow. Moreover, associative therapy promotes marked morphological changes.https://raf.bioscientifica.com/view/journals/raf/2/2/RAF-20-0020.xmlaglepristoneangiogenesisuterusnitric oxidedoppler |
| spellingShingle | Roberto Rodrigues da Rosa Filho Maíra Morales Brito Thaís Gomes Faustino Leticia Lima de Almeida Verônica Correia Manoel Bruno Cogliati Camila Infantosi Vannucchi Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect Reproduction and Fertility aglepristone angiogenesis uterus nitric oxide doppler |
| title | Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect |
| title_full | Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect |
| title_fullStr | Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect |
| title_full_unstemmed | Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect |
| title_short | Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect |
| title_sort | prostaglandin and antigestagen in pyometra bitches vascular and stereological effect |
| topic | aglepristone angiogenesis uterus nitric oxide doppler |
| url | https://raf.bioscientifica.com/view/journals/raf/2/2/RAF-20-0020.xml |
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