DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTS
Background: The heterogeneity of coronavirus disease of 2019 (COVID-19) lies within its diverse symptoms and severity, ranging from mild to lethal. Acute respiratory distress syndrome (ARDS) has been shown to be a leading cause of mortality in COVID-19 patients, characterized by a hyper cytokine st...
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| Format: | Article |
| Language: | English |
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Mattioli1885
2022-10-01
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| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
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| Online Access: | http://mjhid.org/index.php/mjhid/article/view/5112 |
| _version_ | 1797991618837479424 |
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| author | HADI YASSINE |
| author_facet | HADI YASSINE |
| author_sort | HADI YASSINE |
| collection | DOAJ |
| description |
Background: The heterogeneity of coronavirus disease of 2019 (COVID-19) lies within its diverse symptoms and severity, ranging from mild to lethal. Acute respiratory distress syndrome (ARDS) has been shown to be a leading cause of mortality in COVID-19 patients, characterized by a hyper cytokine storm. Autoimmunity is proposed to occur as a result of COVID-19, given the high similarity of the immune responses observed in COVID-19 and autoimmune diseases. Here, we investigate the level of autoimmune antibodies in COVID-19 patients with different severities. Results: Initial screening for antinuclear antibodies (ANA) IgG using ELISA revealed that 1.58% (2/126) and 4% (5/126) of intensive care unit (ICU) COVID-19 cases expressed strong and moderate ANA levels, respectively. An additional sample was positive with immunofluorescence assays (IFA) screening. However, all the non-ICU cases (n=273) were ANA negative using both assays. Samples positive for ANA were further confirmed with large-scale autoantibody screening by phage immunoprecipitation-sequencing (PhIP-Seq). The majority of the ANA-positive samples showed "speckled" ANA pattern by microscopy, and revealed autoantibody specificities that predominantly targeted proteins involved in intracellular signal transduction, metabolism, apoptotic processes, and cell death by PhIP-Seq; further denoting reactivity to nuclear and cytoplasmic antigens. Conclusion: Our results further support the notion of routine screening for autoimmune responses in COVID-19 patients, which might help improve disease prognosis and patient management. Further, results provide compelling evidence that ANA-positive individuals should be excluded from being donors for convalescent plasma therapy in the context of COVID-19.
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| first_indexed | 2024-04-11T08:53:44Z |
| format | Article |
| id | doaj.art-4b20f3cf1edf49dd9f5d7f504226a895 |
| institution | Directory Open Access Journal |
| issn | 2035-3006 |
| language | English |
| last_indexed | 2024-04-11T08:53:44Z |
| publishDate | 2022-10-01 |
| publisher | Mattioli1885 |
| record_format | Article |
| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj.art-4b20f3cf1edf49dd9f5d7f504226a8952022-12-22T04:33:20ZengMattioli1885Mediterranean Journal of Hematology and Infectious Diseases2035-30062022-10-0114110.4084/MJHID.2022.076DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTSHADI YASSINE0a:1:{s:5:"en_US";s:46:"Biomedical Research Center of Qatar University";} Background: The heterogeneity of coronavirus disease of 2019 (COVID-19) lies within its diverse symptoms and severity, ranging from mild to lethal. Acute respiratory distress syndrome (ARDS) has been shown to be a leading cause of mortality in COVID-19 patients, characterized by a hyper cytokine storm. Autoimmunity is proposed to occur as a result of COVID-19, given the high similarity of the immune responses observed in COVID-19 and autoimmune diseases. Here, we investigate the level of autoimmune antibodies in COVID-19 patients with different severities. Results: Initial screening for antinuclear antibodies (ANA) IgG using ELISA revealed that 1.58% (2/126) and 4% (5/126) of intensive care unit (ICU) COVID-19 cases expressed strong and moderate ANA levels, respectively. An additional sample was positive with immunofluorescence assays (IFA) screening. However, all the non-ICU cases (n=273) were ANA negative using both assays. Samples positive for ANA were further confirmed with large-scale autoantibody screening by phage immunoprecipitation-sequencing (PhIP-Seq). The majority of the ANA-positive samples showed "speckled" ANA pattern by microscopy, and revealed autoantibody specificities that predominantly targeted proteins involved in intracellular signal transduction, metabolism, apoptotic processes, and cell death by PhIP-Seq; further denoting reactivity to nuclear and cytoplasmic antigens. Conclusion: Our results further support the notion of routine screening for autoimmune responses in COVID-19 patients, which might help improve disease prognosis and patient management. Further, results provide compelling evidence that ANA-positive individuals should be excluded from being donors for convalescent plasma therapy in the context of COVID-19. http://mjhid.org/index.php/mjhid/article/view/5112AutoimmuneCOVID-19ANAICU |
| spellingShingle | HADI YASSINE DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTS Mediterranean Journal of Hematology and Infectious Diseases Autoimmune COVID-19 ANA ICU |
| title | DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTS |
| title_full | DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTS |
| title_fullStr | DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTS |
| title_full_unstemmed | DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTS |
| title_short | DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTS |
| title_sort | detection of antinuclear antibodies targeting intracellular signal transduction metabolism apoptotic processes and cell death in critical covid 19 patients |
| topic | Autoimmune COVID-19 ANA ICU |
| url | http://mjhid.org/index.php/mjhid/article/view/5112 |
| work_keys_str_mv | AT hadiyassine detectionofantinuclearantibodiestargetingintracellularsignaltransductionmetabolismapoptoticprocessesandcelldeathincriticalcovid19patients |