Accuracy of Techniques for Predicting Gas Production by Ruminants Associated with Diet
The aim of this study was to compare the gas production profiles and nutrient degradability of two diets using automatic and semiautomatic in vitro gas production techniques. A randomized block design in a 2 × 2 factorial arrangement was adopted, with two diets comprising different proportions of no...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/1/39 |
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| author | Juraci M. A. Suassuna Alberício P. de Andrade Daniel R. Menezes Yanna C. F. Teles Cintia M. Araujo Lucas K. S. Lima Patrícia M. G. Beelen André L. R. Magalhães Beatriz D. O. Fernandes Ariosvaldo N. Medeiros |
| author_facet | Juraci M. A. Suassuna Alberício P. de Andrade Daniel R. Menezes Yanna C. F. Teles Cintia M. Araujo Lucas K. S. Lima Patrícia M. G. Beelen André L. R. Magalhães Beatriz D. O. Fernandes Ariosvaldo N. Medeiros |
| author_sort | Juraci M. A. Suassuna |
| collection | DOAJ |
| description | The aim of this study was to compare the gas production profiles and nutrient degradability of two diets using automatic and semiautomatic in vitro gas production techniques. A randomized block design in a 2 × 2 factorial arrangement was adopted, with two diets comprising different proportions of nonfiber carbohydrates (NFCs) (low-NFC vs. high-NFC diets), two gas production measurement techniques (automatic vs. semiautomatic) and four replicates. The blocks represent three in vitro runs. Gas production from the fermentation of fiber carbohydrates (<i>Vf</i>2) was 22% higher when measured with the automatic technique than with the semiautomatic technique. The <i>Vt</i> of the low-NFC diet differed between techniques and was 22.9% higher using the automatic technique. A highly positive correlation (r = 0.96) was observed between the techniques, with a high coefficient of determination between the techniques (R<sup>2</sup> = 0.93). There was greater degradability of dry matter (DMD) and organic matter (OMD) with the automatic technique. In both diets, the degradability of crude protein (CPD) was greater with the semiautomatic technique (<i>p</i> < 0.0001). The high-NFC diet resulted in a lower pH and lower NH<sub>3</sub>-N in the incubation medium than in that of the low-NFC diet, whereas the degradability of DM, OM and CP increased. The automatic and semi-automatic techniques similarly estimated the kinetic parameters and the profiles of total gas production, demonstrating the potential of both techniques for assessing the nutritional value of diets with different proportions of NFCs. |
| first_indexed | 2024-03-09T12:47:54Z |
| format | Article |
| id | doaj.art-4b2231de4ac74fbe89c2e5202e548fd6 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T12:47:54Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-4b2231de4ac74fbe89c2e5202e548fd62023-11-30T22:10:37ZengMDPI AGFermentation2311-56372022-12-01913910.3390/fermentation9010039Accuracy of Techniques for Predicting Gas Production by Ruminants Associated with DietJuraci M. A. Suassuna0Alberício P. de Andrade1Daniel R. Menezes2Yanna C. F. Teles3Cintia M. Araujo4Lucas K. S. Lima5Patrícia M. G. Beelen6André L. R. Magalhães7Beatriz D. O. Fernandes8Ariosvaldo N. Medeiros9Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, BrazilDepartment of Animal Science, Federal University of Agreste of Pernambuco, Garanhuns 55292-270, PE, BrazilDepartment of Veterinary, Federal University of São Francisco Valley, Petrolina 56304-917, PE, BrazilDepartment of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, BrazilDepartment of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, BrazilDepartment of Biological Sciences, State University of Feira de Santana, Feira de Santana 44036-900, BA, BrazilCampus of Engineering and Agricultural Sciences, Federal University of Alagoas, Rio Largo 57100-000, AL, BrazilDepartment of Animal Science, Federal University of Agreste of Pernambuco, Garanhuns 55292-270, PE, BrazilDepartment of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, BrazilDepartment of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, BrazilThe aim of this study was to compare the gas production profiles and nutrient degradability of two diets using automatic and semiautomatic in vitro gas production techniques. A randomized block design in a 2 × 2 factorial arrangement was adopted, with two diets comprising different proportions of nonfiber carbohydrates (NFCs) (low-NFC vs. high-NFC diets), two gas production measurement techniques (automatic vs. semiautomatic) and four replicates. The blocks represent three in vitro runs. Gas production from the fermentation of fiber carbohydrates (<i>Vf</i>2) was 22% higher when measured with the automatic technique than with the semiautomatic technique. The <i>Vt</i> of the low-NFC diet differed between techniques and was 22.9% higher using the automatic technique. A highly positive correlation (r = 0.96) was observed between the techniques, with a high coefficient of determination between the techniques (R<sup>2</sup> = 0.93). There was greater degradability of dry matter (DMD) and organic matter (OMD) with the automatic technique. In both diets, the degradability of crude protein (CPD) was greater with the semiautomatic technique (<i>p</i> < 0.0001). The high-NFC diet resulted in a lower pH and lower NH<sub>3</sub>-N in the incubation medium than in that of the low-NFC diet, whereas the degradability of DM, OM and CP increased. The automatic and semi-automatic techniques similarly estimated the kinetic parameters and the profiles of total gas production, demonstrating the potential of both techniques for assessing the nutritional value of diets with different proportions of NFCs.https://www.mdpi.com/2311-5637/9/1/39Ankom<sup>RF</sup> systemfermentation kineticsshort-chain fatty acids |
| spellingShingle | Juraci M. A. Suassuna Alberício P. de Andrade Daniel R. Menezes Yanna C. F. Teles Cintia M. Araujo Lucas K. S. Lima Patrícia M. G. Beelen André L. R. Magalhães Beatriz D. O. Fernandes Ariosvaldo N. Medeiros Accuracy of Techniques for Predicting Gas Production by Ruminants Associated with Diet Fermentation Ankom<sup>RF</sup> system fermentation kinetics short-chain fatty acids |
| title | Accuracy of Techniques for Predicting Gas Production by Ruminants Associated with Diet |
| title_full | Accuracy of Techniques for Predicting Gas Production by Ruminants Associated with Diet |
| title_fullStr | Accuracy of Techniques for Predicting Gas Production by Ruminants Associated with Diet |
| title_full_unstemmed | Accuracy of Techniques for Predicting Gas Production by Ruminants Associated with Diet |
| title_short | Accuracy of Techniques for Predicting Gas Production by Ruminants Associated with Diet |
| title_sort | accuracy of techniques for predicting gas production by ruminants associated with diet |
| topic | Ankom<sup>RF</sup> system fermentation kinetics short-chain fatty acids |
| url | https://www.mdpi.com/2311-5637/9/1/39 |
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