Construction of the targeted and pH-sensitive paclitaxel drug delivery system RGD/PTX@ZIF-90 and anti-tumor activity research
Tumors area common cause of morbidity and mortality. High treatment efficiency and low drug toxicity are key for effective tumor treatment. Here, the pH-sensitive material ZIF-90 was synthesized by the liquid-phase diffusion method for loading paclitaxel (PTX), and the targeting peptide (RGD) was pr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2021-01-01
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| Series: | Materials Research Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2053-1591/abed89 |
| _version_ | 1827869090987900928 |
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| author | Fei Ge Rui Qiao Ping Song Yugui Tao Longbao Zhu Weiwei Zhang Wanzhen Li Lin Gui |
| author_facet | Fei Ge Rui Qiao Ping Song Yugui Tao Longbao Zhu Weiwei Zhang Wanzhen Li Lin Gui |
| author_sort | Fei Ge |
| collection | DOAJ |
| description | Tumors area common cause of morbidity and mortality. High treatment efficiency and low drug toxicity are key for effective tumor treatment. Here, the pH-sensitive material ZIF-90 was synthesized by the liquid-phase diffusion method for loading paclitaxel (PTX), and the targeting peptide (RGD) was prepared by the solid-phase synthesis method to modify it (RGD/PTX@ZIF-90). The skeleton of RGD/PTX@ZIF-90 collapses in the acidic tumor microenvironment, thereby releasing PTX and mediating the controlled release of the drug. ZIF-90 below 300 nm was obtained by adjusting the ratio of metal ions and organic ligands in the characterization experiment. In addition, in vitro drug release experiments showed that the drug release rate was greater at pH = 5.5 than at pH = 7.4. The lethal rate of RGD/PTX@ZIF-90 to human breast cancer cells (MCF-7) was 44.5%, which was higher than the lethal rate of PTX alone (37.3%) in the cytotoxicity experiment and apoptosis experiment. Uptake experiments revealed that RGD/PTX@ZIF-90 mainly existed in the cytoplasm of MCF-7, which suggests that the drug had successfully entered the cell to achieve the therapeutic effect. The loading of the nano-medicine carrier ZIF-90 and the modification of the targeting peptide RGD significantly improve the therapeutic effect of PTX and indicate that this system could be used to treat breast cancer. |
| first_indexed | 2024-03-12T15:40:04Z |
| format | Article |
| id | doaj.art-4b23210c0a804804874343448a2bd65c |
| institution | Directory Open Access Journal |
| issn | 2053-1591 |
| language | English |
| last_indexed | 2024-03-12T15:40:04Z |
| publishDate | 2021-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Research Express |
| spelling | doaj.art-4b23210c0a804804874343448a2bd65c2023-08-09T16:00:39ZengIOP PublishingMaterials Research Express2053-15912021-01-018404501210.1088/2053-1591/abed89Construction of the targeted and pH-sensitive paclitaxel drug delivery system RGD/PTX@ZIF-90 and anti-tumor activity researchFei Ge0https://orcid.org/0000-0002-6308-3224Rui Qiao1Ping Song2Yugui Tao3https://orcid.org/0000-0002-0144-4872Longbao Zhu4Weiwei Zhang5Wanzhen Li6Lin Gui7College of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, Peoples Republic of ChinaCollege of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, Peoples Republic of ChinaCollege of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, Peoples Republic of ChinaCollege of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, Peoples Republic of ChinaCollege of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, Peoples Republic of ChinaCollege of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, Peoples Republic of ChinaCollege of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, Peoples Republic of ChinaDepartment of Microbiology and Immunology, Wannan Medical College, Wuhu, Anhui 241002, Peoples Republic of ChinaTumors area common cause of morbidity and mortality. High treatment efficiency and low drug toxicity are key for effective tumor treatment. Here, the pH-sensitive material ZIF-90 was synthesized by the liquid-phase diffusion method for loading paclitaxel (PTX), and the targeting peptide (RGD) was prepared by the solid-phase synthesis method to modify it (RGD/PTX@ZIF-90). The skeleton of RGD/PTX@ZIF-90 collapses in the acidic tumor microenvironment, thereby releasing PTX and mediating the controlled release of the drug. ZIF-90 below 300 nm was obtained by adjusting the ratio of metal ions and organic ligands in the characterization experiment. In addition, in vitro drug release experiments showed that the drug release rate was greater at pH = 5.5 than at pH = 7.4. The lethal rate of RGD/PTX@ZIF-90 to human breast cancer cells (MCF-7) was 44.5%, which was higher than the lethal rate of PTX alone (37.3%) in the cytotoxicity experiment and apoptosis experiment. Uptake experiments revealed that RGD/PTX@ZIF-90 mainly existed in the cytoplasm of MCF-7, which suggests that the drug had successfully entered the cell to achieve the therapeutic effect. The loading of the nano-medicine carrier ZIF-90 and the modification of the targeting peptide RGD significantly improve the therapeutic effect of PTX and indicate that this system could be used to treat breast cancer.https://doi.org/10.1088/2053-1591/abed89antitumorpaclitaxelZIF-90RGDpH sensitive |
| spellingShingle | Fei Ge Rui Qiao Ping Song Yugui Tao Longbao Zhu Weiwei Zhang Wanzhen Li Lin Gui Construction of the targeted and pH-sensitive paclitaxel drug delivery system RGD/PTX@ZIF-90 and anti-tumor activity research Materials Research Express antitumor paclitaxel ZIF-90 RGD pH sensitive |
| title | Construction of the targeted and pH-sensitive paclitaxel drug delivery system RGD/PTX@ZIF-90 and anti-tumor activity research |
| title_full | Construction of the targeted and pH-sensitive paclitaxel drug delivery system RGD/PTX@ZIF-90 and anti-tumor activity research |
| title_fullStr | Construction of the targeted and pH-sensitive paclitaxel drug delivery system RGD/PTX@ZIF-90 and anti-tumor activity research |
| title_full_unstemmed | Construction of the targeted and pH-sensitive paclitaxel drug delivery system RGD/PTX@ZIF-90 and anti-tumor activity research |
| title_short | Construction of the targeted and pH-sensitive paclitaxel drug delivery system RGD/PTX@ZIF-90 and anti-tumor activity research |
| title_sort | construction of the targeted and ph sensitive paclitaxel drug delivery system rgd ptx zif 90 and anti tumor activity research |
| topic | antitumor paclitaxel ZIF-90 RGD pH sensitive |
| url | https://doi.org/10.1088/2053-1591/abed89 |
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