Endophytic Fungus from <i>Opuntia ficus-indica:</i> A Source of Potential Bioactive Antimicrobial Compounds against Multidrug-Resistant Bacteria
Endophytic <i>Aspergillus</i> species represent an inexhaustible source for many medicinally important secondary metabolites. The current study isolated the endophytic <i>Aspergillus niger</i> (OL519514) fungus from <i>Opuntia ficus-indica</i> fruit peels. The ant...
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MDPI AG
2022-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/8/1070 |
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| author | Wafaa M. Elkady Marwa M. Raafat Marwa M. Abdel-Aziz Arwa A. AL-Huqail Mohamed L. Ashour Noha Fathallah |
| author_facet | Wafaa M. Elkady Marwa M. Raafat Marwa M. Abdel-Aziz Arwa A. AL-Huqail Mohamed L. Ashour Noha Fathallah |
| author_sort | Wafaa M. Elkady |
| collection | DOAJ |
| description | Endophytic <i>Aspergillus</i> species represent an inexhaustible source for many medicinally important secondary metabolites. The current study isolated the endophytic <i>Aspergillus niger</i> (OL519514) fungus from <i>Opuntia ficus-indica</i> fruit peels. The antibacterial activities were reported for both <i>Aspergillus</i> species and <i>Opuntia ficus-indica</i> fruit peel extract. Extraction of the endophytic fungal metabolites using ethyl acetate and fractionation was performed, yielding dihydroauroglaucin (C1), isotetrahydroauroglaucin (C2), and cristatumin B (C3). Resistant bacterial strains were used to investigate the efficiency of the total fungal ethyl acetate extract (FEA) and the isolated compounds. FEA showed promising wide spectrum activity. (C3) showed excellent activity against selected Gram-negative resistant bacteria; However, (C2) exhibited tremendous activity against the tested Gram-positive resistant strains; conversely, (C1) possessed the lowest antibacterial activity compared to the two other compounds. An in silico virtual molecular docking demonstrated that cristatumin B was the most active antimicrobial compound against the selected protein targets. In conclusion, the active metabolites newly isolated from the endophytic fungus <i>Aspergillus niger</i> (OL519514) and present in plants’ waste can be a promising antimicrobial agent against multidrug-resistant bacteria. |
| first_indexed | 2024-03-09T04:16:55Z |
| format | Article |
| id | doaj.art-4b2507795c1f4e48b2c8498735a29b7e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T04:16:55Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4b2507795c1f4e48b2c8498735a29b7e2023-12-03T13:52:01ZengMDPI AGPlants2223-77472022-04-01118107010.3390/plants11081070Endophytic Fungus from <i>Opuntia ficus-indica:</i> A Source of Potential Bioactive Antimicrobial Compounds against Multidrug-Resistant BacteriaWafaa M. Elkady0Marwa M. Raafat1Marwa M. Abdel-Aziz2Arwa A. AL-Huqail3Mohamed L. Ashour4Noha Fathallah5Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy, Future University in Egypt, Cairo 11835, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Future University in Egypt, Cairo 11835, EgyptRegional Center for Mycology and Biotechnology (RCMB), Al-Azhar University, Cairo 11651, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Abbasia, Cairo 11566, EgyptPharmacognosy and Medicinal Plants Department, Faculty of Pharmacy, Future University in Egypt, Cairo 11835, EgyptEndophytic <i>Aspergillus</i> species represent an inexhaustible source for many medicinally important secondary metabolites. The current study isolated the endophytic <i>Aspergillus niger</i> (OL519514) fungus from <i>Opuntia ficus-indica</i> fruit peels. The antibacterial activities were reported for both <i>Aspergillus</i> species and <i>Opuntia ficus-indica</i> fruit peel extract. Extraction of the endophytic fungal metabolites using ethyl acetate and fractionation was performed, yielding dihydroauroglaucin (C1), isotetrahydroauroglaucin (C2), and cristatumin B (C3). Resistant bacterial strains were used to investigate the efficiency of the total fungal ethyl acetate extract (FEA) and the isolated compounds. FEA showed promising wide spectrum activity. (C3) showed excellent activity against selected Gram-negative resistant bacteria; However, (C2) exhibited tremendous activity against the tested Gram-positive resistant strains; conversely, (C1) possessed the lowest antibacterial activity compared to the two other compounds. An in silico virtual molecular docking demonstrated that cristatumin B was the most active antimicrobial compound against the selected protein targets. In conclusion, the active metabolites newly isolated from the endophytic fungus <i>Aspergillus niger</i> (OL519514) and present in plants’ waste can be a promising antimicrobial agent against multidrug-resistant bacteria.https://www.mdpi.com/2223-7747/11/8/1070<i>Opuntia ficus-indica</i>endophytic <i>Aspergillus niger</i>antimicrobial activitymultidrug-resistant bacteriabenzaldehyde derivativescristatumin B |
| spellingShingle | Wafaa M. Elkady Marwa M. Raafat Marwa M. Abdel-Aziz Arwa A. AL-Huqail Mohamed L. Ashour Noha Fathallah Endophytic Fungus from <i>Opuntia ficus-indica:</i> A Source of Potential Bioactive Antimicrobial Compounds against Multidrug-Resistant Bacteria Plants <i>Opuntia ficus-indica</i> endophytic <i>Aspergillus niger</i> antimicrobial activity multidrug-resistant bacteria benzaldehyde derivatives cristatumin B |
| title | Endophytic Fungus from <i>Opuntia ficus-indica:</i> A Source of Potential Bioactive Antimicrobial Compounds against Multidrug-Resistant Bacteria |
| title_full | Endophytic Fungus from <i>Opuntia ficus-indica:</i> A Source of Potential Bioactive Antimicrobial Compounds against Multidrug-Resistant Bacteria |
| title_fullStr | Endophytic Fungus from <i>Opuntia ficus-indica:</i> A Source of Potential Bioactive Antimicrobial Compounds against Multidrug-Resistant Bacteria |
| title_full_unstemmed | Endophytic Fungus from <i>Opuntia ficus-indica:</i> A Source of Potential Bioactive Antimicrobial Compounds against Multidrug-Resistant Bacteria |
| title_short | Endophytic Fungus from <i>Opuntia ficus-indica:</i> A Source of Potential Bioactive Antimicrobial Compounds against Multidrug-Resistant Bacteria |
| title_sort | endophytic fungus from i opuntia ficus indica i a source of potential bioactive antimicrobial compounds against multidrug resistant bacteria |
| topic | <i>Opuntia ficus-indica</i> endophytic <i>Aspergillus niger</i> antimicrobial activity multidrug-resistant bacteria benzaldehyde derivatives cristatumin B |
| url | https://www.mdpi.com/2223-7747/11/8/1070 |
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