Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina.
The Synaptic Vesicle Protein 2 (SV2) family of transporter-like proteins is expressed exclusively in vesicles that undergo calcium-regulated exocytosis. Of the three isoforms expressed in mammals, SV2B is the most divergent. Here we report studies of SV2B location and function in the retina. Immunol...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2667261?pdf=render |
| _version_ | 1818132486674186240 |
|---|---|
| author | Catherine W Morgans Patricia Kensel-Hammes James B Hurley Kimberly Burton Rejean Idzerda G Stanley McKnight Sandra M Bajjalieh |
| author_facet | Catherine W Morgans Patricia Kensel-Hammes James B Hurley Kimberly Burton Rejean Idzerda G Stanley McKnight Sandra M Bajjalieh |
| author_sort | Catherine W Morgans |
| collection | DOAJ |
| description | The Synaptic Vesicle Protein 2 (SV2) family of transporter-like proteins is expressed exclusively in vesicles that undergo calcium-regulated exocytosis. Of the three isoforms expressed in mammals, SV2B is the most divergent. Here we report studies of SV2B location and function in the retina. Immunolabeling studies revealed that SV2B is detected in rod photoreceptor synaptic terminals where it is the primary isoform. In mice lacking SV2B, synaptic transmission at the synapse between photoreceptors and bipolar neurons was decreased, as evidenced by a significant reduction in the amplitude of the b-wave in electroretinogram recordings. Quantitative immunoblot analyses of whole eyes revealed that loss of SV2B was associated with reduced levels of synaptic vesicle proteins including synaptotagmin, VAMP, synaptophysin and the vesicular glutamate transporter V-GLUT1. Immunolabeling studies revealed that SV2B is detected in rod photoreceptor synaptic terminals where it is the primary isoform. Thus, SV2B contributes to the modulation of synaptic vesicle exocytosis and plays a significant role in regulating synaptic protein content. |
| first_indexed | 2024-12-11T08:37:36Z |
| format | Article |
| id | doaj.art-4b28ed2fa81c40f085348edb49409863 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:37:36Z |
| publishDate | 2009-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4b28ed2fa81c40f085348edb494098632022-12-22T01:14:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0144e523010.1371/journal.pone.0005230Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina.Catherine W MorgansPatricia Kensel-HammesJames B HurleyKimberly BurtonRejean IdzerdaG Stanley McKnightSandra M BajjaliehThe Synaptic Vesicle Protein 2 (SV2) family of transporter-like proteins is expressed exclusively in vesicles that undergo calcium-regulated exocytosis. Of the three isoforms expressed in mammals, SV2B is the most divergent. Here we report studies of SV2B location and function in the retina. Immunolabeling studies revealed that SV2B is detected in rod photoreceptor synaptic terminals where it is the primary isoform. In mice lacking SV2B, synaptic transmission at the synapse between photoreceptors and bipolar neurons was decreased, as evidenced by a significant reduction in the amplitude of the b-wave in electroretinogram recordings. Quantitative immunoblot analyses of whole eyes revealed that loss of SV2B was associated with reduced levels of synaptic vesicle proteins including synaptotagmin, VAMP, synaptophysin and the vesicular glutamate transporter V-GLUT1. Immunolabeling studies revealed that SV2B is detected in rod photoreceptor synaptic terminals where it is the primary isoform. Thus, SV2B contributes to the modulation of synaptic vesicle exocytosis and plays a significant role in regulating synaptic protein content.http://europepmc.org/articles/PMC2667261?pdf=render |
| spellingShingle | Catherine W Morgans Patricia Kensel-Hammes James B Hurley Kimberly Burton Rejean Idzerda G Stanley McKnight Sandra M Bajjalieh Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina. PLoS ONE |
| title | Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina. |
| title_full | Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina. |
| title_fullStr | Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina. |
| title_full_unstemmed | Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina. |
| title_short | Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina. |
| title_sort | loss of the synaptic vesicle protein sv2b results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina |
| url | http://europepmc.org/articles/PMC2667261?pdf=render |
| work_keys_str_mv | AT catherinewmorgans lossofthesynapticvesicleproteinsv2bresultsinreducedneurotransmissionandalteredsynapticvesicleproteinexpressionintheretina AT patriciakenselhammes lossofthesynapticvesicleproteinsv2bresultsinreducedneurotransmissionandalteredsynapticvesicleproteinexpressionintheretina AT jamesbhurley lossofthesynapticvesicleproteinsv2bresultsinreducedneurotransmissionandalteredsynapticvesicleproteinexpressionintheretina AT kimberlyburton lossofthesynapticvesicleproteinsv2bresultsinreducedneurotransmissionandalteredsynapticvesicleproteinexpressionintheretina AT rejeanidzerda lossofthesynapticvesicleproteinsv2bresultsinreducedneurotransmissionandalteredsynapticvesicleproteinexpressionintheretina AT gstanleymcknight lossofthesynapticvesicleproteinsv2bresultsinreducedneurotransmissionandalteredsynapticvesicleproteinexpressionintheretina AT sandrambajjalieh lossofthesynapticvesicleproteinsv2bresultsinreducedneurotransmissionandalteredsynapticvesicleproteinexpressionintheretina |