Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant Acinetobacter baumannii Isolates
Background and Aims: Acinetobacter baumannii is a major cause of nosocomial infections and is resistant to many antibiotics. Over expression of AdeABC and AdeIJK efflux pumps in Acinetobacter causes resistance to aminoglycosides and decreases the sensitivity of fluoroquinolones. The aim of this stu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Farname
2019-01-01
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| Series: | Iranian Journal of Medical Microbiology |
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| Online Access: | http://ijmm.ir/article-1-868-en.html |
| _version_ | 1823938698937368576 |
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| author | Negin Sedighian Kashi Sahar Honarmand Fahimeh Baghbani Arani |
| author_facet | Negin Sedighian Kashi Sahar Honarmand Fahimeh Baghbani Arani |
| author_sort | Negin Sedighian Kashi |
| collection | DOAJ |
| description | Background and Aims: Acinetobacter baumannii is a major cause of nosocomial infections and is resistant to many antibiotics. Over expression of AdeABC and AdeIJK efflux pumps in Acinetobacter causes resistance to aminoglycosides and decreases the sensitivity of fluoroquinolones. The aim of this study was to investigate the phenotypic activity of the Acinetobacter baumanni isolates associated with presence of adeA, adeB, and adeI genes.
Materials and Methods: The study was performed on 55 strains of A. baumannii isolated collected from specimens of patients hospitalized in Milad Hospital , Tehran. The isolates were diagnosed using biochemical tests and antibiotic susceptibility testing was done by disk diffusion method based on CLSI guidelines. Cartwheel method was used to study phenotypic activity of efflux pump. Multiplex PCR was used to determine the presence of genes.
Results: The prevalence of multiple drug resistant isolates was 98%. In terms of efflux pump activity 3.63% isolates were strong, 67.27% moderate and 29.09% were non-active. The frequency of adeA, adeB, adeI genes was 87.2%, 85.4% and 94.5%, respectively. There was significant association between adeA and adeB genes among isolates with actively and non-actively efflux pump.
Conclusions: Determination of actively phenotype of efflux pump can determine the multiple drug resistance among A. baumannii isolates. The results confirms the role of main genes encoding AdeABC operon,adeA and adeB in activity of A. baumannii efflux pump. |
| first_indexed | 2024-12-17T02:49:11Z |
| format | Article |
| id | doaj.art-4b29adf4c35f4c0d8b421135b272e791 |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-12-17T02:49:11Z |
| publishDate | 2019-01-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-4b29adf4c35f4c0d8b421135b272e7912022-12-21T22:06:29ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422019-01-01125301310Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant Acinetobacter baumannii IsolatesNegin Sedighian Kashi0Sahar Honarmand1Fahimeh Baghbani Arani2 Department of Microbiology, Faculty of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran Department of Microbiology, Faculty of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran Department of Genetics and Biotechnology, Faculty of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran Background and Aims: Acinetobacter baumannii is a major cause of nosocomial infections and is resistant to many antibiotics. Over expression of AdeABC and AdeIJK efflux pumps in Acinetobacter causes resistance to aminoglycosides and decreases the sensitivity of fluoroquinolones. The aim of this study was to investigate the phenotypic activity of the Acinetobacter baumanni isolates associated with presence of adeA, adeB, and adeI genes. Materials and Methods: The study was performed on 55 strains of A. baumannii isolated collected from specimens of patients hospitalized in Milad Hospital , Tehran. The isolates were diagnosed using biochemical tests and antibiotic susceptibility testing was done by disk diffusion method based on CLSI guidelines. Cartwheel method was used to study phenotypic activity of efflux pump. Multiplex PCR was used to determine the presence of genes. Results: The prevalence of multiple drug resistant isolates was 98%. In terms of efflux pump activity 3.63% isolates were strong, 67.27% moderate and 29.09% were non-active. The frequency of adeA, adeB, adeI genes was 87.2%, 85.4% and 94.5%, respectively. There was significant association between adeA and adeB genes among isolates with actively and non-actively efflux pump. Conclusions: Determination of actively phenotype of efflux pump can determine the multiple drug resistance among A. baumannii isolates. The results confirms the role of main genes encoding AdeABC operon,adeA and adeB in activity of A. baumannii efflux pump.http://ijmm.ir/article-1-868-en.htmlAcinetobacter baumanniEfflux pumpadeAadeBadeI genesMultiplex PCR |
| spellingShingle | Negin Sedighian Kashi Sahar Honarmand Fahimeh Baghbani Arani Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant Acinetobacter baumannii Isolates Iranian Journal of Medical Microbiology Acinetobacter baumanni Efflux pump adeA adeB adeI genes Multiplex PCR |
| title | Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant
Acinetobacter baumannii Isolates |
| title_full | Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant
Acinetobacter baumannii Isolates |
| title_fullStr | Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant
Acinetobacter baumannii Isolates |
| title_full_unstemmed | Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant
Acinetobacter baumannii Isolates |
| title_short | Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant
Acinetobacter baumannii Isolates |
| title_sort | investigation of association between adea adeb adei genes and phenotypic activity of efflux pump in multiple drug resistant acinetobacter baumannii isolates |
| topic | Acinetobacter baumanni Efflux pump adeA adeB adeI genes Multiplex PCR |
| url | http://ijmm.ir/article-1-868-en.html |
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