Ginsenoside Re protects rhinovirus-induced disruption of tight junction through inhibition of ROS-mediated phosphatases inactivation in human nasal epithelial cells
Maintaining tight junction integrity significantly contributes to epithelial barrier function. If the barrier function is destroyed, the permeability of the cells increases, and the movement of the pathogens is promoted, thereby further increasing the susceptibility to secondary infection. Ginsenosi...
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024037198 |
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| author | Kyeong Ah Kim Joo Hyun Jung Yun Sook Choi Seon Tae Kim |
| author_facet | Kyeong Ah Kim Joo Hyun Jung Yun Sook Choi Seon Tae Kim |
| author_sort | Kyeong Ah Kim |
| collection | DOAJ |
| description | Maintaining tight junction integrity significantly contributes to epithelial barrier function. If the barrier function is destroyed, the permeability of the cells increases, and the movement of the pathogens is promoted, thereby further increasing the susceptibility to secondary infection. Ginsenoside components have multiple biological activities, including antiviral effects. In this study, we examined the protective effects of ginsenoside Re against rhinovirus-induced tight junction disruption in primary human nasal epithelial cells (HNE). Incubation with human rhinovirus resulted in marked disruption of tight junction proteins (ZO-1, E-cadherin, claudin-1, and occludin) in human nasal epithelial cells. Rhinovirus-induced disruption of tight junction proteins was strongly inhibited by the treatment of cells with ginsenoside Re. Indeed, significant amounts of reactive oxygen species (ROS) have been detected in human nasal epithelial cells co-incubated with rhinovirus. Moreover, rhinovirus-induced ROS generation was markedly reduced by the ginsenoside Re. However, ginsenosides Rb1 and Rc did not inhibit tight junction disruption or ROS generation in nasal epithelial cells following incubation with rhinovirus. Furthermore, incubation with rhinovirus resulted in a marked decrease in protein phosphatase activity and an increase in protein tyrosine phosphorylation levels in nasal epithelial cells. Treatment of cells with ginsenoside Re inhibited rhinovirus-induced inactivation of phosphatases and phosphorylation of tyrosine. Our results identified ginsenoside Re as an effective compound that prevented rhinovirus-induced tight junction disruption in human nasal epithelial cells. |
| first_indexed | 2024-04-24T23:13:35Z |
| format | Article |
| id | doaj.art-4b2eceb76d104565aa97effea5d19262 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:13:35Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-4b2eceb76d104565aa97effea5d192622024-03-17T07:58:22ZengElsevierHeliyon2405-84402024-03-01105e27688Ginsenoside Re protects rhinovirus-induced disruption of tight junction through inhibition of ROS-mediated phosphatases inactivation in human nasal epithelial cellsKyeong Ah Kim0Joo Hyun Jung1Yun Sook Choi2Seon Tae Kim3Department of Otolaryngology-Head & Neck Surgery, Gachon University Gil Medical Center, Incheon, South KoreaDepartment of Otolaryngology-Head & Neck Surgery, Gachon University Gil Medical Center, Incheon, South KoreaDepartment of Otolaryngology-Head & Neck Surgery, Gachon University Gil Medical Center, Incheon, South KoreaCorresponding author. Department of Otolaryngology-Head & Neck Surgery, Gachon University Gil Medical Center, Gachon University of Medicine and Science, 21 Namdong-daero 774beon-gil, Namdong-gu, Incheon, 21565, South Korea.; Department of Otolaryngology-Head & Neck Surgery, Gachon University Gil Medical Center, Incheon, South KoreaMaintaining tight junction integrity significantly contributes to epithelial barrier function. If the barrier function is destroyed, the permeability of the cells increases, and the movement of the pathogens is promoted, thereby further increasing the susceptibility to secondary infection. Ginsenoside components have multiple biological activities, including antiviral effects. In this study, we examined the protective effects of ginsenoside Re against rhinovirus-induced tight junction disruption in primary human nasal epithelial cells (HNE). Incubation with human rhinovirus resulted in marked disruption of tight junction proteins (ZO-1, E-cadherin, claudin-1, and occludin) in human nasal epithelial cells. Rhinovirus-induced disruption of tight junction proteins was strongly inhibited by the treatment of cells with ginsenoside Re. Indeed, significant amounts of reactive oxygen species (ROS) have been detected in human nasal epithelial cells co-incubated with rhinovirus. Moreover, rhinovirus-induced ROS generation was markedly reduced by the ginsenoside Re. However, ginsenosides Rb1 and Rc did not inhibit tight junction disruption or ROS generation in nasal epithelial cells following incubation with rhinovirus. Furthermore, incubation with rhinovirus resulted in a marked decrease in protein phosphatase activity and an increase in protein tyrosine phosphorylation levels in nasal epithelial cells. Treatment of cells with ginsenoside Re inhibited rhinovirus-induced inactivation of phosphatases and phosphorylation of tyrosine. Our results identified ginsenoside Re as an effective compound that prevented rhinovirus-induced tight junction disruption in human nasal epithelial cells.http://www.sciencedirect.com/science/article/pii/S2405844024037198Ginsenoside ReHuman rhinovirusROSHuman nasal epithelial cellsTight junction proteins |
| spellingShingle | Kyeong Ah Kim Joo Hyun Jung Yun Sook Choi Seon Tae Kim Ginsenoside Re protects rhinovirus-induced disruption of tight junction through inhibition of ROS-mediated phosphatases inactivation in human nasal epithelial cells Heliyon Ginsenoside Re Human rhinovirus ROS Human nasal epithelial cells Tight junction proteins |
| title | Ginsenoside Re protects rhinovirus-induced disruption of tight junction through inhibition of ROS-mediated phosphatases inactivation in human nasal epithelial cells |
| title_full | Ginsenoside Re protects rhinovirus-induced disruption of tight junction through inhibition of ROS-mediated phosphatases inactivation in human nasal epithelial cells |
| title_fullStr | Ginsenoside Re protects rhinovirus-induced disruption of tight junction through inhibition of ROS-mediated phosphatases inactivation in human nasal epithelial cells |
| title_full_unstemmed | Ginsenoside Re protects rhinovirus-induced disruption of tight junction through inhibition of ROS-mediated phosphatases inactivation in human nasal epithelial cells |
| title_short | Ginsenoside Re protects rhinovirus-induced disruption of tight junction through inhibition of ROS-mediated phosphatases inactivation in human nasal epithelial cells |
| title_sort | ginsenoside re protects rhinovirus induced disruption of tight junction through inhibition of ros mediated phosphatases inactivation in human nasal epithelial cells |
| topic | Ginsenoside Re Human rhinovirus ROS Human nasal epithelial cells Tight junction proteins |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024037198 |
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