Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation
Lingonberry (<i>Vaccinium vitis-idaea</i> L.) is an important and valuable horticultural crop due to its high antioxidant properties. Plant tissue culture is an advanced propagation system employed in horticultural crops. However, the progeny derived using this technique may not be true-...
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MDPI AG
2023-07-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/8/397 |
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| author | Umanath Sharma Arindam Sikdar Abir U. Igamberdiev Samir C. Debnath |
| author_facet | Umanath Sharma Arindam Sikdar Abir U. Igamberdiev Samir C. Debnath |
| author_sort | Umanath Sharma |
| collection | DOAJ |
| description | Lingonberry (<i>Vaccinium vitis-idaea</i> L.) is an important and valuable horticultural crop due to its high antioxidant properties. Plant tissue culture is an advanced propagation system employed in horticultural crops. However, the progeny derived using this technique may not be true-to-type. In order to obtain the maximum return of any agricultural enterprise, uniformity of planting materials is necessary, which sometimes is not achieved due to genetic and epigenetic instabilities under in vitro culture. Therefore, we analyzed morphological traits and genetic and epigenetic variations under tissue-culture and greenhouse conditions in lingonberry using molecular markers. Leaf length and leaf width under greenhouse conditions and shoot number per explant, shoot height and shoot vigor under in vitro conditions were higher in hybrid H1 compared to the cultivar Erntedank. Clonal fidelity study using one expressed sequence tag (EST)—polymerase chain reaction (PCR), five EST—simple sequence repeat (SSR) and six genomic (G)—SSR markers revealed monomorphic bands in micropropagated shoots and plants in lingonberry hybrid H1 and cultivar Erntedank conforming genetic integrity. Epigenetic variation was studied by quantifying cytosine methylation using a methylation-sensitive amplification polymorphism (MSAP) technique. DNA methylation ranged from 32% in greenhouse-grown hybrid H1 to 44% in cultivar Erntedank under a tissue culture system. Although total methylation was higher in in vitro grown shoots, fully methylated bands were observed more in the greenhouse-grown plants. On the contrary, hemimethylated DNA bands were more prominent in tissue culture conditions as compared to the greenhouse-grown plants. The study conclude that lingonberry maintains its genetic integrity but undergoes variable epigenetic changes during in vitro and ex vitro conditions. |
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| language | English |
| last_indexed | 2024-03-11T00:02:08Z |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-4b308bcd727b44be8fc5c2f7f39e60c12023-11-19T00:41:37ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-07-014586296631010.3390/cimb45080397Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal PropagationUmanath Sharma0Arindam Sikdar1Abir U. Igamberdiev2Samir C. Debnath3Department of Biology, Memorial University of Newfoundland, 45 Arctic Avenue, St. John’s, NL A1C 5S7, CanadaDepartment of Biology, Memorial University of Newfoundland, 45 Arctic Avenue, St. John’s, NL A1C 5S7, CanadaDepartment of Biology, Memorial University of Newfoundland, 45 Arctic Avenue, St. John’s, NL A1C 5S7, CanadaSt. John’s Research and Development Centre, Agriculture and Agri-Food Canada, 204 Brookfield Road, St. John’s, NL A1E 0B2, CanadaLingonberry (<i>Vaccinium vitis-idaea</i> L.) is an important and valuable horticultural crop due to its high antioxidant properties. Plant tissue culture is an advanced propagation system employed in horticultural crops. However, the progeny derived using this technique may not be true-to-type. In order to obtain the maximum return of any agricultural enterprise, uniformity of planting materials is necessary, which sometimes is not achieved due to genetic and epigenetic instabilities under in vitro culture. Therefore, we analyzed morphological traits and genetic and epigenetic variations under tissue-culture and greenhouse conditions in lingonberry using molecular markers. Leaf length and leaf width under greenhouse conditions and shoot number per explant, shoot height and shoot vigor under in vitro conditions were higher in hybrid H1 compared to the cultivar Erntedank. Clonal fidelity study using one expressed sequence tag (EST)—polymerase chain reaction (PCR), five EST—simple sequence repeat (SSR) and six genomic (G)—SSR markers revealed monomorphic bands in micropropagated shoots and plants in lingonberry hybrid H1 and cultivar Erntedank conforming genetic integrity. Epigenetic variation was studied by quantifying cytosine methylation using a methylation-sensitive amplification polymorphism (MSAP) technique. DNA methylation ranged from 32% in greenhouse-grown hybrid H1 to 44% in cultivar Erntedank under a tissue culture system. Although total methylation was higher in in vitro grown shoots, fully methylated bands were observed more in the greenhouse-grown plants. On the contrary, hemimethylated DNA bands were more prominent in tissue culture conditions as compared to the greenhouse-grown plants. The study conclude that lingonberry maintains its genetic integrity but undergoes variable epigenetic changes during in vitro and ex vitro conditions.https://www.mdpi.com/1467-3045/45/8/397DNA methylationepigenetic variationgreenhouse-grown plantsin vitro culturemolecular markersshoot proliferation |
| spellingShingle | Umanath Sharma Arindam Sikdar Abir U. Igamberdiev Samir C. Debnath Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation Current Issues in Molecular Biology DNA methylation epigenetic variation greenhouse-grown plants in vitro culture molecular markers shoot proliferation |
| title | Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation |
| title_full | Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation |
| title_fullStr | Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation |
| title_full_unstemmed | Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation |
| title_short | Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation |
| title_sort | exploring genetic and epigenetic changes in lingonberry using molecular markers implications for clonal propagation |
| topic | DNA methylation epigenetic variation greenhouse-grown plants in vitro culture molecular markers shoot proliferation |
| url | https://www.mdpi.com/1467-3045/45/8/397 |
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