Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensis
After programmed cell death (PCD), heartwood formation, storage, and processing, wood DNA degradation occurs to varying degrees. The concentration and distribution of nuclei and plastids in xylem cells of Cunninghamia lanceolata and Aquilaria sinensis, treated under different conditions of processin...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2015-01-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_1304_Zhang_Xylem_Cunninghamia_Aquilaria |
| _version_ | 1818417772703514624 |
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| author | Rong Zhang Kuiwu Xu Kelin Ye |
| author_facet | Rong Zhang Kuiwu Xu Kelin Ye |
| author_sort | Rong Zhang |
| collection | DOAJ |
| description | After programmed cell death (PCD), heartwood formation, storage, and processing, wood DNA degradation occurs to varying degrees. The concentration and distribution of nuclei and plastids in xylem cells of Cunninghamia lanceolata and Aquilaria sinensis, treated under different conditions of processing and storing, were studied by analyzing the distribution frequency, area, and signal intensity, in specimens that had been stained with aceto-carmine, DAPI, and I2-KI. Most of the nuclei and plastids were present in the ray cells, and a small quantity of nuclei and plastids were present in the axial parenchyma cells. There was an indication that the concentration of the remaining nuclei and plastids in the xylem cells was mainly affected by the xylem heartwood formation, storage time, and temperature. The nuclei and plastids content of the sapwood was greater than that of the heartwood. However, the nuclei and plastids content of the fresh wood was greater than that of the processed and stored wood. An estimation of the quantity of nuclei and plastids using staining methods could provide a direct basis for the appropriate selection of a procedure for DNA extraction. |
| first_indexed | 2024-12-14T12:12:06Z |
| format | Article |
| id | doaj.art-4b30cb6b6b1a49718b837ec25f65af43 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-14T12:12:06Z |
| publishDate | 2015-01-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-4b30cb6b6b1a49718b837ec25f65af432022-12-21T23:01:43ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-01-011011304131710.15376/biores.10.1.1304-1317Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensisRong Zhang0Kuiwu Xu1Kelin Ye2College of Materials Science and Engineering, Nanjing Forestry University, No.159 Longpan Road, Nanjing, China; ChinaCollege of Materials Science and Engineering, Nanjing Forestry University, No.159 Longpan Road, Nanjing, China; ChinaResearch Institute of Wood Industry, Chinese Academy of Forestry, No.1 Dongxiaofu, Beijing, China; ChinaAfter programmed cell death (PCD), heartwood formation, storage, and processing, wood DNA degradation occurs to varying degrees. The concentration and distribution of nuclei and plastids in xylem cells of Cunninghamia lanceolata and Aquilaria sinensis, treated under different conditions of processing and storing, were studied by analyzing the distribution frequency, area, and signal intensity, in specimens that had been stained with aceto-carmine, DAPI, and I2-KI. Most of the nuclei and plastids were present in the ray cells, and a small quantity of nuclei and plastids were present in the axial parenchyma cells. There was an indication that the concentration of the remaining nuclei and plastids in the xylem cells was mainly affected by the xylem heartwood formation, storage time, and temperature. The nuclei and plastids content of the sapwood was greater than that of the heartwood. However, the nuclei and plastids content of the fresh wood was greater than that of the processed and stored wood. An estimation of the quantity of nuclei and plastids using staining methods could provide a direct basis for the appropriate selection of a procedure for DNA extraction.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_1304_Zhang_Xylem_Cunninghamia_AquilariaPlastidXylemNucleiAceto-carmineDAPII2-KI |
| spellingShingle | Rong Zhang Kuiwu Xu Kelin Ye Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensis BioResources Plastid Xylem Nuclei Aceto-carmine DAPI I2-KI |
| title | Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensis |
| title_full | Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensis |
| title_fullStr | Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensis |
| title_full_unstemmed | Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensis |
| title_short | Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensis |
| title_sort | concentration and distribution of nuclei and plastids in xylem cells in cunninghamia lanceolata and aquilaria sinensis |
| topic | Plastid Xylem Nuclei Aceto-carmine DAPI I2-KI |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_1304_Zhang_Xylem_Cunninghamia_Aquilaria |
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