Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots
Lipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and...
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MDPI AG
2021-10-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/11/10/762 |
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| author | Guzel R. Akhiyarova Ruslan S. Ivanov Igor I. Ivanov Ekaterina I. Finkina Daria N. Melnikova Ivan V. Bogdanov Tatyana Nuzhnaya Tatiana V. Ovchinnikova Dmitriy S. Veselov Guzel R. Kudoyarova |
| author_facet | Guzel R. Akhiyarova Ruslan S. Ivanov Igor I. Ivanov Ekaterina I. Finkina Daria N. Melnikova Ivan V. Bogdanov Tatyana Nuzhnaya Tatiana V. Ovchinnikova Dmitriy S. Veselov Guzel R. Kudoyarova |
| author_sort | Guzel R. Akhiyarova |
| collection | DOAJ |
| description | Lipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and their mutual participation in suberin deposition in root endodermis of salt-stressed pea plants. Using immunohistochemistry we show for the first time NaCl induced accumulation of LTPs and ABA in the cell walls of phloem paralleled by suberin deposition in the endoderm region of pea roots. Unlike LTPs which were found localized around phloem cells, ABA was also present within phloem cells. In addition, ABA treatment resulted in both LTP and ABA accumulation in phloem cells and promoted root suberization. These results suggested the importance of NaCl-induced accumulation of ABA in increasing the abundance of LTPs and of suberin. Using molecular modeling and fluorescence spectroscopy we confirmed the ability of different plant LTPs, including pea Ps-LTP1, to bind ABA. We therefore hypothesize an involvement of plant LTPs in ABA transport (unloading from phloem) as part of the salinity adaptation mechanism. |
| first_indexed | 2024-03-10T06:23:53Z |
| format | Article |
| id | doaj.art-4b33a93aa5104673a01e292f68f0fe4d |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-10T06:23:53Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-4b33a93aa5104673a01e292f68f0fe4d2023-11-22T19:06:14ZengMDPI AGMembranes2077-03752021-10-01111076210.3390/membranes11100762Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea RootsGuzel R. Akhiyarova0Ruslan S. Ivanov1Igor I. Ivanov2Ekaterina I. Finkina3Daria N. Melnikova4Ivan V. Bogdanov5Tatyana Nuzhnaya6Tatiana V. Ovchinnikova7Dmitriy S. Veselov8Guzel R. Kudoyarova9Ufa Federal Research Centre, Ufa Institute of Biology, Russian Academy of Sciences 450054, pr. Octyabrya, 69, 450054 Ufa, RussiaUfa Federal Research Centre, Ufa Institute of Biology, Russian Academy of Sciences 450054, pr. Octyabrya, 69, 450054 Ufa, RussiaUfa Federal Research Centre, Ufa Institute of Biology, Russian Academy of Sciences 450054, pr. Octyabrya, 69, 450054 Ufa, RussiaM. M. Shemyakin & Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow, RussiaM. M. Shemyakin & Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow, RussiaM. M. Shemyakin & Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow, RussiaUfa Federal Research Centre, Ufa Institute of Biology, Russian Academy of Sciences 450054, pr. Octyabrya, 69, 450054 Ufa, RussiaM. M. Shemyakin & Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow, RussiaUfa Federal Research Centre, Ufa Institute of Biology, Russian Academy of Sciences 450054, pr. Octyabrya, 69, 450054 Ufa, RussiaUfa Federal Research Centre, Ufa Institute of Biology, Russian Academy of Sciences 450054, pr. Octyabrya, 69, 450054 Ufa, RussiaLipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and their mutual participation in suberin deposition in root endodermis of salt-stressed pea plants. Using immunohistochemistry we show for the first time NaCl induced accumulation of LTPs and ABA in the cell walls of phloem paralleled by suberin deposition in the endoderm region of pea roots. Unlike LTPs which were found localized around phloem cells, ABA was also present within phloem cells. In addition, ABA treatment resulted in both LTP and ABA accumulation in phloem cells and promoted root suberization. These results suggested the importance of NaCl-induced accumulation of ABA in increasing the abundance of LTPs and of suberin. Using molecular modeling and fluorescence spectroscopy we confirmed the ability of different plant LTPs, including pea Ps-LTP1, to bind ABA. We therefore hypothesize an involvement of plant LTPs in ABA transport (unloading from phloem) as part of the salinity adaptation mechanism.https://www.mdpi.com/2077-0375/11/10/762garden pea<i>Pisum sativum</i>lipid transfer proteins (LTPs)plant rootsplant hormonesabscisic acid (ABA) |
| spellingShingle | Guzel R. Akhiyarova Ruslan S. Ivanov Igor I. Ivanov Ekaterina I. Finkina Daria N. Melnikova Ivan V. Bogdanov Tatyana Nuzhnaya Tatiana V. Ovchinnikova Dmitriy S. Veselov Guzel R. Kudoyarova Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots Membranes garden pea <i>Pisum sativum</i> lipid transfer proteins (LTPs) plant roots plant hormones abscisic acid (ABA) |
| title | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
| title_full | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
| title_fullStr | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
| title_full_unstemmed | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
| title_short | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
| title_sort | effects of salinity and abscisic acid on lipid transfer protein accumulation suberin deposition and hydraulic conductance in pea roots |
| topic | garden pea <i>Pisum sativum</i> lipid transfer proteins (LTPs) plant roots plant hormones abscisic acid (ABA) |
| url | https://www.mdpi.com/2077-0375/11/10/762 |
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